LEADER 05343nam 22006734a 450 001 9910954523603321 005 20200520144314.0 010 $a9786612118302 010 $a9781282118300 010 $a1282118307 010 $a9781444313086 010 $a1444313088 035 $a(CKB)1000000000766489 035 $a(EBL)437488 035 $a(OCoLC)429316204 035 $a(SSID)ssj0000333932 035 $a(PQKBManifestationID)11259081 035 $a(PQKBTitleCode)TC0000333932 035 $a(PQKBWorkID)10396966 035 $a(PQKB)10185130 035 $a(Au-PeEL)EBL437488 035 $a(CaPaEBR)ebr10307411 035 $a(CaONFJC)MIL211830 035 $a(MiAaPQ)EBC437488 035 $a(Perlego)2750216 035 $a(EXLCZ)991000000000766489 100 $a20020509d2003 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aAdvanced genetic analysis $efinding meaning in a genome /$fR. Scott Hawley and Michelle Y. Walker 205 $a1st ed. 210 $aMalden, Mass. $cBlackwell Pub.$dc2003 215 $a1 online resource (257 p.) 300 $aDescription based upon print version of record. 311 08$a9781405103367 311 08$a1405103361 320 $aIncludes bibliographical references (p. [220]-235) and indexes. 327 $aAdvanced Genetic Analysis; Contents; Preface; Introduction; 1: Mutation; 1.1 Types of mutations; 1.1.1 Muller's classification of mutants; 1.1.2 Modern mutant terminology; 1.1.3 DNA-level terminology; 1.2 Dominance and recessivity; 1.2.1 Dominance and recessivity at the level of the cell; 1.2.2 Difficulties in applying the terms ""dominant"" and ""recessive"" to sex-linked mutants; 1.3 The genetic utility of dominant and recessive mutants; Summary; Gallery of model organisms; 1.1 Our favorite organism: Drosophila melanogaster; 1.2 Our second favorite organism: Saccharomyces cerevisiae 327 $a1.3 Our third favorite organism: Caenorhabditis elegans1.4 Our new favorite organism: zebrafish; 1.5 Phage lambda; 1.6 Phage T4; 1.7 Arabidopsis thaliana; 1.8 Mus musculus (the mouse); 2: Mutant hunts; 2.1 Why look for new mutant?; 2.1.1 Reason 1: To identify genes required for a specific biological process; Box 2.1 A screen for embryonic lethal mutations in Drosophila; Box 2.2 The balancer chromosome; 2.1.2 Reason 2: To isolate more mutations in a specific gene of interest; Box 2.3 A screen for sex-linked lethal mutations in Drosophila 327 $a2.1.3 Reason 3: To obtain mutation tools for structure-function analysis2.1.4 Reason 4: To isolate mutations in a gene so far identified only by molecular approaches; 2.2 Mutagenesis and mutational mechanisms; 2.2.1 Method 1: Ionizing radiation (usually X-rays and gamma-rays); 2.2.2 Method 2: Chemical mutagens; 2.2.3 Method 3: Transposons as mutagens; 2.2.4 Method 4: Targeted gene disruption (a variant on transposon mutagenesis); Box 2.4 Making phenocopies by RNAi and co-suppression; 2.3 What phenotype should you screen (or select) for?; 2.4 Actually getting started 327 $a2.4.1 Your starting material2.4.2 Pilot screens; 2.4.3 Keeping too many, keeping too few; 2.4.4 How many mutants is enough?; Summary; Box 2.5 Reviews of mutant isolation schemes and techniques in various organisms; 3: The complementation test; 3.1 The essence of the complementation test; Box 3.1 A more rigorous definition of the complementation test; Box 3.2 An example of using the complementation test in yeast; 3.2 Rules for using the complementation test; Box 3.3 Transformation rescue is a variant of the complementation test 327 $aBox 3.4 One method for determining whether or not a dominant mutation is an allele of a given gene, or how to make dominants into recessives by pseudo-reversion3.3 How might the complementation test lie to you?; 3.4 Second-site non-complementation (SSNC) (non-allelic non-complementation); 3.4.1 Type I SSNC (poisonous interactions): the interaction is allele-specific at both loci; Box 3.5 Pairing-dependent complementation: transvection; Box 3.6 Synthetic lethality and genetic buffering; 3.4.2 Type 2 SSNC (sequestration): the interaction is allele-specific at one locus 327 $a3.4.3 Type 3 SSNC (combined haplo-insufficiency): the interaction is allele-independent at both loci 330 $aAdvanced Genetic Analysis brings a state-of-the-art, exciting new approach to genetic analysis. Focusing on the underlying principles of modern genetic analysis, this book provides the 'how' and 'why' of the essential analytical tools needed. The author's vibrant, accessible style provides an easy guide to difficult genetic concepts, from mutation and gene function to gene mapping and chromosome segregation. Throughout, a balanced range of model organisms and timely examples are used to illustrate the theoretical basics. Basic principles - Focuses students attention on t 606 $aGenetics$xResearch$xMethodology 615 0$aGenetics$xResearch$xMethodology. 676 $a576.5/072 700 $aHawley$b R. Scott$01810218 701 $aWalker$b Michelle Y$01810219 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910954523603321 996 $aAdvanced genetic analysis$94361450 997 $aUNINA