LEADER 04438nam 22006255 450 001 9910920447403321 005 20260327113212.0 010 $a9789819768516 010 $a9819768519 024 7 $a10.1007/978-981-97-6851-6 035 $a(MiAaPQ)EBC31867299 035 $a(Au-PeEL)EBL31867299 035 $a(CKB)37111014700041 035 $a(DE-He213)978-981-97-6851-6 035 $a(EXLCZ)9937111014700041 100 $a20241230d2024 u| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aAntibiotics and Their Mechanisms of Action /$fedited by Deepesh Nagarajan 205 $a1st ed. 2024. 210 1$aSingapore :$cSpringer Nature Singapore :$cImprint: Springer,$d2024. 215 $a1 online resource (185 pages) 311 08$a9789819768509 311 08$a9819768500 327 $aChapter 1. The history of antibiotics -- Chapter 2. Methods for antibiotic discovery -- Chapter 3. Penicillin -- Chapter 4. Vancomycin -- Chapter 5. Antimicrobial peptides -- Chapter 6. Quinolones -- Chapter 7. Rifampicin -- Chapter 8. Tetracyclines -- Chapter 9. Streptomycin -- Chapter 10. Chloramphenicol -- Chapter 11. Antimetabolites -- Chapter 12. Mycolic acid inhibitors -- Chapter 13. Other antimicrobial agents -- Chapter 14. Antibiotic Pharmacology and Biopharmaceutics. 330 $a?Antibiotics and their mechanisms of action? provides a deep and lucid understanding of the mechanisms of action of various antibiotics. The experiments followed to decipher the mechanisms have been explained in great depth, which is a novelty in this textbook. Most undergraduate textbooks on antibiotics place emphasis on their use from the clinician?s perspective. Taking penicillin as an example, typical textbooks discuss its discovery, penicillin?s ?-lactam derivatives, routes of administration, the diseases and pathogens treated with ?-lactam antibiotics, side effects, and finally the mechanisms of resistance that have evolved against penicillin. No description of how its mechanism of action was deciphered is ever provided. This textbook describes how simple experiments using protoplasts, covalent affinity chromatography, SYPRO Orange binding assays, and X-ray crystallography have all been used to elucidate the mechanism of action of penicillin. Like this, the complicated mechanisms of action of different antibiotics have been presented in simple terms with the help of original illustrations. Chapters 8-10 cover the mechanisms of action of anti-ribosomal antibiotics (tetracycline, streptomycin, chloramphenicol). Elucidating the mechanisms of action for these antibiotics requires complex experiments such as ex vivo transcription via a cell-free ribosomal reaction mixture, photolabelling experiments, and sucrose density gradient centrifugation of radiolabelled translation reactants and products. These experiments are conveyed to the reader in the simplest possible language and with easily understandable, appealing illustrations. Finally, the reader is challenged at the end of every chapter with original questions, testing whether hecan apply the concepts he learned in the preceding chapter to research-level problems. This book is written primarily for undergraduate students in microbiology or biotechnology courses to help them develop a simplistic yet comprehensive understanding of the mechanisms underlying antibiotics action. It is believed that this book will prepare the students for PhD level research. 606 $aPharmacology 606 $aMedical microbiology 606 $aDrug delivery systems 606 $aPharmacology 606 $aMedical Microbiology 606 $aDrug Delivery 606 $aAntibiòtics$2thub 606 $aMecanismes de reacció (Química)$2thub 608 $aLlibres electrònics$2thub 615 0$aPharmacology. 615 0$aMedical microbiology. 615 0$aDrug delivery systems. 615 14$aPharmacology. 615 24$aMedical Microbiology. 615 24$aDrug Delivery. 615 7$aAntibiòtics 615 7$aMecanismes de reacció (Química) 676 $a615 700 $aNagarajan$b Deepesh$01781745 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910920447403321 996 $aAntibiotics and Their Mechanisms of Action$94306566 997 $aUNINA