LEADER 05121nam 22006014a 450 001 9910877256003321 005 20200520144314.0 010 $a1-280-85417-0 010 $a9786610854172 010 $a3-527-60718-8 010 $a3-527-60709-9 035 $a(CKB)1000000000375907 035 $a(EBL)481947 035 $a(OCoLC)70113389 035 $a(SSID)ssj0000147565 035 $a(PQKBManifestationID)11157739 035 $a(PQKBTitleCode)TC0000147565 035 $a(PQKBWorkID)10009725 035 $a(PQKB)10968214 035 $a(MiAaPQ)EBC481947 035 $a(EXLCZ)991000000000375907 100 $a20060908d2006 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aEngineering the genetic code $eexpanding the amino acid repertoire for the design of novel proteins /$fNediljko Budisa 210 $aWeinheim $cWiley-VCH$dc2006 215 $a1 online resource (314 p.) 300 $aDescription based upon print version of record. 311 $a3-527-31243-9 320 $aIncludes bibliographical references and index. 327 $aEngineering the Genetic Code; Foreword; Contents; Preface; 1 Introduction; 1.1 Classical Approaches to Protein Modification; 1.2 Peptide Synthesis, Semisynthesis and Chemistry of Total Protein Synthesis; 1.3 Chemoselective Ligations Combined with Biochemical Methods; 1.4 Methods and Approaches of Classical Protein Engineering; 1.5 Genetically Encoded Protein Modifications - Reprogramming Protein Translation; 1.6 Basic Definitions and Taxonomy; References; 2 A Brief History of an Expanded Amino Acid Repertoire; 2.1 The ""Golden Years"" of Molecular Biology and Triplet Code Elucidation 327 $a2.2 Early Experiments on the Incorporation of Amino Acid Analogs in Proteins2.3 Test Tube (Cell-free) Synthesis of Proteins and Early Incorporation Experiments; 2.4 Noncanonical Amino Acids as Tools for Studying Cell Metabolism, Physiology, Protein Processing and Turnover; 2.5 Problem of Proofs and Formal Criteria for Noncanonical Amino Acid Incorporation; 2.6 Recent Renaissance - Genetic Code Engineering; References; 3 Basic Features of the Cellular Translation Apparatus; 3.1 Natural Laws, Genetic Information and the ""Central Dogma"" of Molecular Biology 327 $a3.2 Cellular Investments in Ribosome-mediated Protein Synthesis3.3 Molecular Architecture of AARS; 3.4 Structure and Function of the tRNA Molecule; 3.5 Aminoacylation Reaction; 3.6 AARS:tRNA Interactions - Identity Sets; 3.7 Translational Proofreading; 3.8 Ribosomal Decoding - A Brief Overview; 3.9 Codon Bias and the Fidelity of Protein Synthesis; 3.10 Preprogrammed Context-dependent Recoding: fMet, Sec, Pyl, etc.; 3.11 Beyond Basic Coding - Posttranslational Modifications; References; 4 Amino Acids and Codons - Code Organization and Protein Structure 327 $a4.1 Basic Features and Adaptive Nature of the Universal Genetic Code4.2 Metabolism and Intracellular Uptake of Canonical Amino Acids; 4.3 Physicochemical Properties of Canonical Amino Acids; 4.4 Reasons for the Occurrence of Only 20 Amino Acids in the Genetic Code; 4.5 What Properties of Amino Acids are Best Preserved by the Genetic Code?; 4.6 Evolutionary Legacy: Dual Nature of Conserved Code and Finite Number of Protein Folds; 4.7 Natural Variations in Assignment of Codons of the Universal Genetic Code; 4.7.1 Nucleoside Modifications and Codon Reassignments 327 $a4.8 Codon Reassignment Concepts Possibly Relevant to Code Engineering4.8.1 Genome Size, Composition, Complexity and Codon Reassignments; 4.8.2 Stop Codon Takeover, Codon Capture and Codon Ambiguity; References; 5 Reprograming the Cellular Translation Machinery; 5.1 Enzyme Specificity of Aminoacyl-tRNA Synthetases (AARS) and Code Interpretation; 5.1.1 Living Cells as Platforms for Amino Acid Repertoire Expansion; 5.1.2 Uptake, Toxicity and Metabolic Fate of Noncanonical Amino Acids; 5.1.2.1 General Considerations; 5.1.2.2 Amino Acid Transport 327 $a5.1.2.3 Metabolic Conversions and Toxicity of Analogs and Surrogates 330 $aThe ability to introduce non-canonical amino acids in vivo has greatly expanded the repertoire of accessible proteins for basic research and biotechnological application.Here, the different methods and strategies to incorporate new or modified amino acids are explained in detail, including a lot of practical advice for first-time users of this powerful technique.Novel applications in protein biochemistry, genomics, biotechnology and biomedicine made possible by the expansion of the genetic code are discussed and numerous examples are given.Essential reading for all molecular life s 606 $aProtein engineering 606 $aAmino acids$xSynthesis 615 0$aProtein engineering. 615 0$aAmino acids$xSynthesis. 676 $a660.6/3 700 $aBudisa$b Nediljko$01755976 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910877256003321 996 $aEngineering the genetic code$94193019 997 $aUNINA