LEADER 03370oam 2200793zu 450 001 9910877017303321 005 20230607215334.0 010 $a1-280-55837-7 010 $a9786610558377 010 $a3-527-60064-7 035 $a(CKB)1000000000019305 035 $a(SSID)ssj0000294044 035 $a(PQKBManifestationID)11222947 035 $a(PQKBTitleCode)TC0000294044 035 $a(PQKBWorkID)10303276 035 $a(PQKB)10668017 035 $a(MiAaPQ)EBC4956603 035 $a(Au-PeEL)EBL4956603 035 $a(CaONFJC)MIL55837 035 $a(OCoLC)1024267738 035 $a(EXLCZ)991000000000019305 100 $a20160829d2002 uy 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt 182 $cc 183 $acr 200 00$aDirected molecular evolution of proteins : or how to improve enzymes for biocatalysis 210 31$a[Place of publication not identified]$cWiley VCH$d2002 215 $a1 online resource (359 pages) 300 $aBibliographic Level Mode of Issuance: Monograph 311 $a3-527-30423-1 330 $aNatural selection created optimal catalysts. However, optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio- and enantioselectivity. The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity. 606 $aEnzymes$xBiotechnology 606 $aBiocatalysis$xEvolution 606 $aProteins$xChemical modification 606 $aProteins 606 $aMolecular evolution 606 $aCombinatorial chemistry 606 $aDirected Molecular Evolution 606 $aProtein Engineering 606 $aGenetic Engineering 606 $aGenetic Techniques 606 $aInvestigative Techniques 606 $aAnalytical, Diagnostic and Therapeutic Techniques and Equipment 606 $aBiomedical Engineering$2HILCC 606 $aHealth & Biological Sciences$2HILCC 615 0$aEnzymes$xBiotechnology 615 0$aBiocatalysis$xEvolution 615 0$aProteins$xChemical modification 615 0$aProteins 615 0$aMolecular evolution 615 0$aCombinatorial chemistry 615 2$aDirected Molecular Evolution 615 2$aProtein Engineering 615 2$aGenetic Engineering 615 2$aGenetic Techniques 615 2$aInvestigative Techniques 615 2$aAnalytical, Diagnostic and Therapeutic Techniques and Equipment 615 7$aBiomedical Engineering 615 7$aHealth & Biological Sciences 676 $a660.6/34 702 $aBrakmann$b Susanne 702 $aJohnsson$b Kai 801 0$bPQKB 906 $aBOOK 912 $a9910877017303321 996 $aDirected molecular evolution of proteins : or how to improve enzymes for biocatalysis$94189314 997 $aUNINA