LEADER 05122nam 2200637Ia 450 001 9910876609703321 005 20200520144314.0 010 $a1-282-02547-3 010 $a9786612025471 010 $a3-527-62383-3 010 $a3-527-62384-1 035 $a(CKB)1000000000707314 035 $a(EBL)481743 035 $a(SSID)ssj0000204251 035 $a(PQKBManifestationID)11175460 035 $a(PQKBTitleCode)TC0000204251 035 $a(PQKBWorkID)10174965 035 $a(PQKB)10883314 035 $a(MiAaPQ)EBC481743 035 $a(OCoLC)476313693 035 $a(EXLCZ)991000000000707314 100 $a20080902d2009 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aModern biocatalysis $estereoselective and environmentally friendly reactions /$fedited by Wolf-Dieter Fessner and Thorleif Anthonsen 210 $aWeinheim $cWiley-VCH$dc2009 215 $a1 online resource (401 p.) 300 $aDescription based upon print version of record. 311 $a3-527-32071-7 320 $aIncludes bibliographical references and index. 327 $aModern Biocatalysis; Contents; Preface; List of Contributors; 1: Fluorescence Assays for Biotransformations; 1.1 Introduction; 1.2 Alcohol Dehydrogenases (ADHs) and Aldolases; 1.2.1 Chiral Fluorogenic ADH Substrates; 1.2.2 Fluorogenic Aldolase Probes; 1.2.3 Transaldolases and Transketolases; 1.2.4 Enolase Probe; 1.3 Lipases and Esterases; 1.3.1 Assays on Solid Support; 1.3.2 The Clips-O Substrates with Periodate; 1.3.3 Esters of Fluorogenic Cyanohydrins and Hydroxyketones; 1.3.4 Fluorogenic Acyloxymethyl Ethers; 1.3.5 FRET-Lipase Probes; 1.4 Other Hydrolases; 1.4.1 Epoxide Hydrolases 327 $a1.4.2 Amidases and Proteases1.4.3 Phosphatases; 1.5 Baeyer-Villigerases; 1.6 Conclusion; Acknowledgment; References; 2: Immobilization as a Tool for Improving Enzymes; 2.1 Introduction; 2.2 Adsorption/Electrostatic Interactions; 2.2.1 Van der Waals Interactions; 2.2.2 Hydrogen Bonds; 2.2.3 Ionic Interactions; 2.3 Encapsulation; 2.4 Covalent Binding/Cross-linking; 2.5 Conclusion; Acknowledgments; References; 3: Continuous-flow Microchannel Reactors with Surface-immobilized Biocatalysts; 3.1 Introduction 327 $a3.2 Biocatalytic Synthesis Using Microreaction Technology with Free and Immobilized Enzymes3.3 Novel Microfluidic Immobilized Enzyme Reactors; 3.3.1 Microreactor Design; 3.3.2 Enzyme Immobilization; 3.4 Enzymatic Hydrolysis of Lactose; 3.4.1 Catalytic Effectiveness of Immobilized CelB; 3.4.2 Continuous Conversion of Lactose; 3.5 Biocatalytic Process Intensification Using Microreaction Technology; 3.6 Conclusions and Outlook; Acknowledgements; References; 4: Activity and Stability of Proteases in Hydrophilic Solvents; 4.1 Introduction 327 $a4.2 Activity and Selectivity of Proteases in Synthesis of Carbohydrate Fatty Acid Esters4.3 Enzyme Stability and Conformation; 4.4 Solvent Engineering; 4.5 Conclusion; References; 5: Importance of Enzyme Formulation for the Activity and Enantioselectivity of Lipases in Organic Solvents; 5.1 Introduction; 5.2 Lipase Formulations and their Activity and Enantioselectivity in Neat Organic Solvent; 5.3 Why do Additives Affect the Activity and Enantioselectivity of Lipases in Organic Solvent?; 5.4 Conclusions; References 327 $a6: Direct Esterification with Dry Mycelia of Molds: a (Stereo)selective, Mild and Efficient Method for Obtaining Structurally Diverse Esters6.1 Mycelia and Biotransformations in Organic Media; 6.2 Screening and Microbiological Aspects; 6.3 Production of Acetates; 6.4 Stereoselective Esterifications of Racemic Alcohols; 6.5 Stereoselective Esterifications of Racemic Carboxylic Acids; 6.6 Partition Phenomena and Equilibrium of Esterification Reactions; 6.7 Conclusions; References; 7: Factors Affecting Enantioselectivity: Allosteric Effects; 7.1 How to Provide Enantiopure Compounds 327 $a7.1.1 Kinetic Resolution of Racemic Mixtures Catalyzed by Enzymes 330 $aThis reference covers the wide and rapidly growing field of biocatalysis. It combines complementary expertise from such areas as microbiology, enzymology, molecular biology structural biology and organic chemistry, thus highlighting the interdisciplinary nature of the subject.With its special focus on progress and new developments towards environmentally beneficial reactions with high levels of selectivity for the production of key compound classes, this book will enlighten both chemists and biologists as to the advances and opportunities existing in enzyme catalysis. 606 $aEnzymes$xBiotechnology 606 $aCatalysis 615 0$aEnzymes$xBiotechnology. 615 0$aCatalysis. 676 $a660.634 686 $a58.30$2bcl 686 $a35.17$2bcl 701 $aFessner$b W.-D$g(Wolf-Dieter)$0100520 701 $aAnthonsen$b Thorleif$01763011 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910876609703321 996 $aModern biocatalysis$94203245 997 $aUNINA