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024 7 $a10.1007/978-3-031-51171-4
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101 0 $aeng
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181   $ctxt$2rdacontent
182   $cc$2rdamedia
183   $acr$2rdacarrier
200 10$aCryo-Electron Tomography $eStructural Biology in situ /$fedited by Friedrich Förster, Ariane Briegel
205   $a1st ed. 2024.
210  1$aCham :$cSpringer International Publishing :$cImprint: Springer,$d2024.
215   $a1 online resource (391 pages)
225 1 $aFocus on Structural Biology,$x2542-9566 ;$v11
311 08$a9783031511707 
327   $a1. Fundamentals of Instrumentation and electron optics For cryo-electron tomography -- 2. Principles of tomographic reconstruction  -- 3. Cryo-preparation for cellular tomography -- 4. Cryo-FIB milling -- 5. In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques -- 6. Labeling approaches and correlation with light microscopy (Cryo) CLEM -- 7. Data collection -- 8. Subvolume averaging and classification -- 9. High resolution tomography/ Molecular model building and visualization -- 10. Beam-induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electron Tomography -- 11. Tomogram segmentation -- 12. Electron tomography of cryo-fixed and resin-embedded samples -- 13. Democratization of tomographic data / FAIR data.
330   $aThis book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facilitate such research, cryo-ET is a key method to visualize the molecules of life in their native settings. Cryo-ET enables the imaging of samples that are preserved in a near-native state, at (macro)-molecular resolution and in three dimensions. Thus, this technique is a unique tool to gain insights into how biomolecules collaborate in orchestrating fundamental biological processes, how mutations cause diseases, pathogens cause infections, and to develop novel therapeutics to treat such illnesses. This book provides a unique reference for the emerging field of cryo-ET. The topics covered range from the fundamental principles of imaging to sample preparation, data analysis, and data sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics, and molecular biology and highly valuable for the more experienced and specialized PhD student. Furthermore, it stands as a state-of-the?art source of knowledge for the established senior scientist within the field of structural biology.
410  0$aFocus on Structural Biology,$x2542-9566 ;$v11
606   $aBiomolecules
606   $aPhysical biochemistry
606   $aMacromolecules
606   $aBiology$xTechnique
606   $aBiophysics
606   $aImmunology
606   $aMaterials$xMicroscopy
606   $aStructural Biology
606   $aBiophysical Methods
606   $aImmunology
606   $aMicroscopy
615  0$aBiomolecules.
615  0$aPhysical biochemistry.
615  0$aMacromolecules.
615  0$aBiology$xTechnique.
615  0$aBiophysics.
615  0$aImmunology.
615  0$aMaterials$xMicroscopy.
615 14$aStructural Biology.
615 24$aBiophysical Methods.
615 24$aImmunology.
615 24$aMicroscopy.
676   $a616.0757
700   $aFörster$b Friedrich$0561355
701   $aBriegel$b Ariane$01737786
801  0$bMiAaPQ
801  1$bMiAaPQ
801  2$bMiAaPQ
912   $a9910855369203321
996   $aCryo-Electron Tomography$94159707
997   $aUNINA