LEADER 04684nam 22004815 450 001 9910845077903321 005 20240329120138.0 010 $a3-031-52193-5 024 7 $a10.1007/978-3-031-52193-5 035 $a(MiAaPQ)EBC31224749 035 $a(Au-PeEL)EBL31224749 035 $a(DE-He213)978-3-031-52193-5 035 $a(CKB)31051666900041 035 $a(EXLCZ)9931051666900041 100 $a20240320d2024 u| 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aAdvanced Technologies for Protein Complex Production and Characterization$b[electronic resource] $eVolume II /$fedited by M. Cristina Vega, Francisco J. Fernández 205 $a1st ed. 2024. 210 1$aCham :$cSpringer Nature Switzerland :$cImprint: Springer,$d2024. 215 $a1 online resource (216 pages) 225 1 $aAdvances in Experimental Medicine and Biology,$x2214-8019 ;$v1453 311 $a3-031-52192-7 327 $aChapter 1: Immunoprecipitation methods to isolate messenger ribonucleoprotein complexes (mRNP) -- Chapter 2: Purification of in vivo or in vitro-assembled RNA-protein complexes by RNA centric methods -- Chapter 3: Peptide-based mass spectrometry for protein complexes investigation -- Chapter 4: Probing protein complexes composition, stoichiometry, and interactions by peptide-based mass spectrometry -- Chapter 5: Discovery and examination of linear motif mediated protein-protein complexes -- Chapter 6: Protein-protein binding kinetics by biolayer interferometry -- Chapter 7: Studying Macromolecular Interactions of Cellular Machines by the Combined use of Analytical Ultracentrifugation, Light Scattering, and Fluorescence Spectroscopy Methods -- Chapter 8: The complementarity of nuclear magnetic resonance and native mass spectrometry in probing protein-protein interactions -- Chapter 9: X-ray crystallography for protein complexes -- Chapter 10: Characterizationof biological samples using ultra-short and ultra-bright XFEL pulses -- Chapter 11: Small-angle X-ray scattering for macromolecular complexes -- Chapter 12: Sample Preparation for Electron Cryo-Microscopy of Macromolecular Machines -- Chapter 13: Characterization of Complexes and Supramolecular Structures by Electron Microscopy. 330 $aThis edited volume discusses the identification, discovery, characterization, structure determination and modeling of multicomponent macromolecular complexes, and as such, it fully complements the first volume (ISBN 978-3-319-27214-6), which targeted methods of recombinant production of protein complexes. This book is divided in 8 sections offering a selection of technologies widely used in the characterization of protein and protein-nucleic acid complexes for different purposes and at different scales. From native electrophoresis methods, that are accessible to any reasonably well-equipped laboratory, to the sophisticated setup required for structure determination by cryo-electron microscopy or X-ray crystallography, this book contains a wide variety of clearly explained analytic and preparative approaches, connected with the production techniques developed on the previous volume. The readers will find an integral connection between this book and the first volume, which ensures a comprehensive and updated discussion of the main topics of the discipline. Taken together, these volumes constitute a cohesive and authoritative source of the research on multicomponent macromolecular complexes. In here, we focus on characterization of protein complexes in the broadest sense, which is not typically covered in other sources. Moreover, all chapters are carefully written by world renowned scientists and active researchers, making this volume ideal, not only as a reference source, but also as a companion book for the daily laboratory work. This book is aimed for a wide range of scientists, from science students to experienced researchers, working on protein and protein-nucleic acid complexes, who need a thorough understanding of protein production and complex characterization. . 410 0$aAdvances in Experimental Medicine and Biology,$x2214-8019 ;$v1453 606 $aLife sciences 606 $aLife Sciences 615 0$aLife sciences. 615 14$aLife Sciences. 676 $a570 700 $aVega$b M. Cristina$01734526 701 $aFernández$b Francisco J$01734527 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910845077903321 996 $aAdvanced Technologies for Protein Complex Production and Characterization$94151692 997 $aUNINA