LEADER 05383nam 2200637Ia 450 001 9910831061403321 005 20230617035332.0 010 $a1-280-51972-X 010 $a9786610519729 010 $a3-527-60367-0 010 $a3-527-60441-3 035 $a(CKB)1000000000377108 035 $a(EBL)482012 035 $a(OCoLC)68572295 035 $a(SSID)ssj0000228314 035 $a(PQKBManifestationID)11208614 035 $a(PQKBTitleCode)TC0000228314 035 $a(PQKBWorkID)10148558 035 $a(PQKB)11020992 035 $a(MiAaPQ)EBC482012 035 $a(EXLCZ)991000000000377108 100 $a20040928d2005 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aProduction of recombinant proteins$b[electronic resource] $enovel microbial and eukaryotic expression systems /$fedited by Gerd Gellissen 210 $aWeinheim $cWiley-VCH$dc2005 215 $a1 online resource (432 p.) 300 $aDescription based upon print version of record. 311 $a3-527-31036-3 320 $aIncludes bibliographical references and index. 327 $aProduction of Recombinant Proteins; Preface; Foreword; Contents; List of Contributors; 1 Key and Criteria to the Selection of an Expression Platform; 2 Escherichia coli; 2.1 Introduction; 2.2 Strains, Genome, and Cultivation; 2.3 Expression Vectors; 2.3.1 Replication of pMB1-derived Vectors; 2.3.2 Plasmid Partitioning; 2.3.3 Genome Engineering; 2.3.4 E. coli Promoters; 2.4 Regulation of Gene Expression; 2.4.1 Negative Control; 2.4.2 Positive Control; 2.4.2.1 L-Arabinose Operon; 2.4.2.2 L-Rhamnose Operon; 2.5 Transcription and Translation; 2.5.1 Translation Initiation; 2.5.2 Codon Usage 327 $a2.5.3 Translation Termination2.5.4 Transcription Termination and mRNA Stability; 2.6 Protein Production; 2.6.1 Inclusion Body Formation; 2.6.1.1 Chaperones as Facilitators of Folding; 2.6.1.2 Fusion Protein Technology; 2.6.2 Methionine Processing; 2.6.3 Secretion into the Periplasm; 2.6.4 Disulfide Bond Formation and Folding; 2.6.5 Twin Arginine Translocation (TAT) of Folded Proteins; 2.6.6 Disulfide Bond Formation in the Cytoplasm; 2.6.7 Cell Surface Display and Secretion across the Outer Membrane; 2.7 Examples of Products and Processes; 2.8 Conclusions and Future Perspectives; Appendix 327 $aReferences3 Pseudomonas fluorescens; 3.1 Introduction; 3.2 Biology of Pseudomonas fluorescens; 3.3 History and Taxonomy of Pseudomonas fluorescens Strain Biovar I MB101; 3.4 Cultivation; 3.5 Genomics and Functional Genomics of P. fluorescens Strain MB101; 3.6 Core Expression Platform for Heterologous Proteins; 3.6.1 Antibiotic-free Plasmids using pyrF and proC; 3.6.2 Gene Deletion Strategy and Re-usable Markers; 3.6.3 Periplasmic Secretion and Use of Transposomes; 3.6.4 Alternative Expression Systems: Anthranilate and Benzoate-inducible Promoters 327 $a3.7 Production of Heterologous Proteins in P. fluorescens3.7.1 Pharmaceutical Proteins; 3.7.2 Industrial Enzymes; 3.7.3 Agricultural Proteins; 3.8 Conclusions; Appendix; References; 4 Staphylococcus carnosus and other Gram-positive Bacteria; 4.1 Introduction; 4.2 Major Protein Export Routes in Gram-positive Bacteria; 4.2.1 The General Secretion (Sec) Pathway; 4.2.2 The Twin-Arginine Translocation (Tat) Pathway; 4.2.3 Secretion Signals; 4.3 Extracytosolic Protein Folding; 4.4 The Cell Wall as a Barrier for the Secretion of Heterologous Proteins 327 $a4.5 Degradation of Exported Proteins by Cell-associated and Secreted Proteases4.6 Staphylococcus carnosus; 4.6.1 General Description; 4.6.2 Microbiological and Molecular Biological Tools; 4.6.3 S. carnosus as Host Organism for the Analysis of Staphylococcal-related Pathogenicity Aspects; 4.6.4 Secretory Production of Heterologous Proteins by S. carnosus; 4.6.4.1 The Staphylococcus hyicus Lipase: Secretory Signals and Heterologous Expression in S. carnosus; 4.6.4.2 Use of the Pre-pro-part of the S. hyicus Lipase for the Secretion of Heterologous Proteins in S. carnosus 327 $a4.6.4.3 Process Development for the Secretory Production of a Human Calcitonin Precursor Fusion Protein by S. carnosus 330 $aWhile the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section -- with strains, genetic elements, vectors and special methods, where applicable -- as well as examples of proteins produced wi 606 $aRecombinant proteins 606 $aRecombinant microorganisms 606 $aGenetic vectors 615 0$aRecombinant proteins. 615 0$aRecombinant microorganisms. 615 0$aGenetic vectors. 676 $a660.63 701 $aGellissen$b Gerd$01603739 712 02$aWiley Online Library (Servicio en línea) 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910831061403321 996 $aProduction of recombinant proteins$93928252 997 $aUNINA