LEADER 01905nam 2200373 n 450 001 996394969403316 005 20200824121117.0 035 $a(CKB)3810000000013785 035 $a(EEBO)2240912427 035 $a(UnM)ocm99891560e 035 $a(UnM)99891560 035 $a(EXLCZ)993810000000013785 100 $a19910725d1575 uy 101 0 $aeng 135 $aurbn||||a|bb| 200 10$a[Pr]incipia L[at]ine loquendi, scribendique siue, Selecta quĉdam ex Ciceronis epistolis$b[electronic resource] $ead pueros in Latina lingua exercendos, adiecta interpretatione Anglica, & (vbi opus esse visum est) Latina declaratione. Ad rationem quam nuper suis sedulitate summa Gallice? conscripsit. Maturinus Corderius. A very necessary and profitable entraunce to the speakyng and writyng of the Latin tongue. Or a certain draught taken out of Ciceroes epistles, for the exercise of children in the Latin speache together with an easy and a familiar construction thereof into Englishe. Opusculum ad conuertendam Latini sermonis puritatem in nostri vernaculi vsum & familiaritatem literario tyrocinio apprime accomodum, gratum, iuxta ac vtile. /$fTranslated by T.W 210 $a[S.l. $cs.n.]$dAnno d[omi]ni. 1575 215 $a[1]+ p 300 $aFragment: title page only. 300 $aReproduction of original in the British Library. 330 $aeebo-0018 606 $aLatin language$xInstruction and study$vEarly works to 1800 615 0$aLatin language$xInstruction and study 700 $aCicero$b Marcus Tullius$082411 701 $aCordier$b Mathurin$f1479-1564.$0796382 702 $aT. W$ffl. 1573-1595, 801 0$bCu-RivES 801 1$bCu-RivES 801 2$bCStRLIN 801 2$bCu-RivES 906 $aBOOK 912 $a996394969403316 996 $aIncipia Latine loquendi, scribendique siue, Selecta quĉdam ex Ciceronis epistolis$92311340 997 $aUNISA LEADER 00885nam a22002411i 4500 001 991002887119707536 005 20040415071837.0 008 040624s1980 it |||||||||||||||||ita 035 $ab12992951-39ule_inst 035 $aARCHE-095063$9ExL 040 $aDip.to Beni Culturali$bita$cA.t.i. Arché s.c.r.l. Pandora Sicilia s.r.l. 082 04$a937.8009 100 1 $aAnello, Pietrina$0487707 245 10$aDionisio il Vecchio /$cPietrina Anello 260 $aPalermo :$b[s.n.],$c1980- 300 $av. ;$c21 cm 600 04$aDionisio$c(Tiranno di Siracusa ;$b1.) 907 $a.b12992951$b02-04-14$c12-07-04 912 $a991002887119707536 945 $aLE001 SG IV 30$g1$i2001000038817$lle001$nV. 1. - C. 1$o-$pE0.00$q-$rl$s- $t0$u3$v0$w3$x0$y.i13598454$z12-07-04 996 $aDionisio il Vecchio$9284570 997 $aUNISALENTO 998 $ale001$b12-07-04$cm$da $e-$fita$git $h0$i1 LEADER 05184nam 2200637Ia 450 001 9910831037603321 005 20170810175612.0 010 $a1-282-29205-6 010 $a9786612292057 010 $a3-527-62668-9 010 $a3-527-62669-7 035 $a(CKB)1000000000767195 035 $a(EBL)481485 035 $a(OCoLC)437416793 035 $a(SSID)ssj0000137586 035 $a(PQKBManifestationID)11159659 035 $a(PQKBTitleCode)TC0000137586 035 $a(PQKBWorkID)10088690 035 $a(PQKB)11399282 035 $a(MiAaPQ)EBC481485 035 $a(PPN)194575683 035 $a(EXLCZ)991000000000767195 100 $a20080107d2009 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aDetection of highly dangerous pathogens$b[electronic resource] $emicroarray methods for BSL 3 and BSL 4 agents /$fedited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel 210 $aWeinheim $cWiley-VCH$d2009 215 $a1 online resource (193 p.) 300 $aDescription based upon print version of record. 311 $a3-527-32275-2 320 $aIncludes bibliographical references and index. 327 $aDetection of Highly Dangerous Pathogens: Microarray Methods for the Detection of BSL 3 and BSL 4 Agents; Contents; This Publication is Supported by COST; Preface; List of Contributors; 1 Introduction to Microarray-Based Detection Methods; 1.1 Introduction to Microarray Technology; 1.2 Technical Aspects of Microarray Technology; 1.2.1 Probes; 1.2.1.1 Genome Fragments; 1.2.1.2 PCR Products; 1.2.1.3 Oligonucleotide Probes; 1.2.2 Substrates for Printing; 1.2.2.1 Slides with Poly-L-lysine Coating; 1.2.2.2 Slides with Amino Silane Coating; 1.2.2.3 Slides with Aldehyde Coating 327 $a1.2.2.4 Slides with Epoxy Coating1.2.2.5 Proprietary Surface Chemistries; 1.2.2.6 Probe Spacers; 1.2.3 Targets for Microarray Analysis; 1.2.3.1 Target Amplifications and Sensitivity Issues; 1.2.3.2 Labeling of the Targets; 1.2.3.3 Hybridization and Wash Conditions; 1.2.4 Classical Commercially Available Microarray Formats; 1.2.4.1 Spotting Approaches; 1.2.4.2 In Situ Synthesis; 1.2.5 Alternative Methods for Improving Microarray-Based Detection Sensitivity; 1.2.5.1 Resonance-Light Scattering (RLS); 1.2.5.2 Planar-Waveguide Technology (PWT); 1.2.5.3 Liquid Arrays 327 $a1.2.5.4 Three-Dimensional Microarray Formats1.2.6 Marker Genes Used on MDMs; 1.3 Analysis and Quality Control Aspects; 1.4 Applications of Microarray Technology in Microbial Diagnostics; 1.4.1 Gene Expression Studies; 1.4.2 Comparative Genomic Hybridization (CGH); 1.4.3 Generic or Universal Microarrays; 1.4.4 Microarrays for Sequence Analysis; 1.4.5 Microbial Diagnostic Microarrays (MDMs); 1.5 Further Developments and New Perspectives Regarding Array Sensitivity and Specificity; 1.6 Conclusions; References; Part I: Methods; 2 Long Oligonucleotide Microarray-Based Microbial Detection 327 $a2.1 Introduction2.2 Method; 2.2.1 DNA Extraction; 2.2.2 ?29 Amplification; 2.2.3 Klenow Amplification/Labeling; 2.2.4 Probe and Slide Preparation; 2.2.5 Slide Processing Protocol (for Amino Surfaces); 2.2.6 Hybridization and Slide Washing; 2.2.7 Comments; 2.3 Our Test System and Results; 2.4 Conclusions; References; 3 Sequence-Specific End-Labeling of Oligonucleotides; 3.1 Introduction; 3.2 Probe Design; 3.3 Slide Preparation (Spotting); 3.4 Slide Processing Protocol ( for Aldehyde Surfaces); 3.5 DNA Extraction and PCR Amplification of the Targeted Gene 327 $a3.6 Shrimp Alkaline Phosphatase Treatment3.7 Labeling; 3.8 Hybridization and Slide Washing; 3.9 Data Analysis; 3.10 Costs; 3.11 Microarray for Detection of Pathogenic Bacteria; References; 4 Non-Cognate Approaches for Pathogen Detection on Microarrays; 4.1 Introduction; 4.2 Non-Cognate Hybridization System; 4.2.1 Concept; 4.2.2 Definition of the Optimal Probe Length; 4.2.3 Virtual Assessment of Array Performances (in Silico Experiments); 4.2.4 Array Manufacturing and Hybridization (Wet-Lab Experiments); 4.2.5 Analysis; 4.3 Perspectives; References; 5 Patterning Techniques for Array Platforms 327 $a5.1 Introduction 330 $aWritten by leading experts in the field as part of an interdisciplinary pan-European research program funded by the EU, the results provided in this booklet provide a unique and comprehensive overview of how microarray technology can be used in safely tracking the most highly dangerous pathogens. A must-have for public health agencies focused on bioterrorism as well as all laboratories working with BSL3 and/or BSL 4 agents. 606 $aPathogenic microorganisms$xDetection 606 $aDNA microarrays 615 0$aPathogenic microorganisms$xDetection. 615 0$aDNA microarrays. 676 $a579.165 701 $aKostic$b Tanja$01628649 701 $aButaye$b Patrick$01628650 701 $aSchrenzel$b Jacques$01628651 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910831037603321 996 $aDetection of highly dangerous pathogens$93965893 997 $aUNINA