LEADER 05647nam 2200673Ia 450 001 9910830959103321 005 20230331005752.0 010 $a1-282-23726-8 010 $a9786612237263 010 $a1-4443-1414-9 010 $a1-4443-1413-0 035 $a(CKB)1000000000789606 035 $a(EBL)454308 035 $a(SSID)ssj0000354244 035 $a(PQKBManifestationID)11264305 035 $a(PQKBTitleCode)TC0000354244 035 $a(PQKBWorkID)10302625 035 $a(PQKB)10134140 035 $a(MiAaPQ)EBC454308 035 $a(OCoLC)501316383 035 $a(EXLCZ)991000000000789606 100 $a19900524d1991 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aGenetic manipulation $etechniques and applications /$fedited by J.M. Grange, A. Fox & N.L. Morgan 210 $aOxford ;$aBoston $cBlackwell Scientific Publications$d1991 215 $a1 online resource (417 p.) 225 1 $aThe Society for Applied Bacteriology technical series ;$vno. 28 300 $a"Based on the Society of Applied Bacteriology Autumn Meeting held at the South Bank Polytechnic, London, on 18 October 1989"--Pref. 311 $a0-632-02926-9 320 $aIncludes bibliographical references and index. 327 $aGenetic Manipulation: Techniques and Applications; Contents; Contributors; Preface; Extraction, Purification and Assay of DNA; Isolation of DNA; Isolation of Plasmid DNA from Bacteria; Purification of DNA; Isolating Genomic DNA From Bacterial Cells; Isolating Mammalian DNA; Assay of DNA; References; Restriction Mapping of DNA; Restriction Enzymes; Approaches to Mapping; References; The Construction and Use of Cloning Vectors; The Commercially Available Vectors; The Construction of Vectors for Use with the Clostridia; References; Appendix I: Addresses of Suppliers of Cloning Vectors 327 $aAppendix II: Addresses of Suppliers of Other Products Useful for Molecular BiologyAdaptor Based cDNA Cloning in the Phage Vectors lgt10 and lgt11; Experimental Methods; Results of cDNA Library Analysis; Discussion; Acknowledgements; References; Plasmid Profiling and DNA/DNA Hybridization for Distinguishing Between Mesophilic Aeromonas Bacteria; DNA/DNA Hybridization; Plasmid Profiling; Methods; Results; Acknowledgements; References; Appendix: Reagents; Preparation and Screening of Bacterial Genomic Libraries; General Strategy; Construction of a Genomic Library by using lEMBL4 327 $aScreening of a lEMBL4 Library with Gene ProbesConstruction of a lgt11 Library and Screening with Antibodies; Acknowledgements; References; DNA Probes for Detection and Identification of Bacteria; Principle of Nucleic Acid Hybridization; Application of DNA Hybridization; The Polymerase Chain Reaction; References; Extraction and Purification of Eukaryotic mRNA; Basic Precautions Prior to RNA Extraction; RNA Extraction Protocols; Analysis of Purified RNA; Isolation of Polyadenylated mRNA from Total RNA; Storage of RNA Samples; Acknowledgements; References; cDNA Cloning 327 $aHow Many Clones are Required?Cloning Strategies; First-strand Synthesis; Second-strand Synthesis; Preparation of the cDNA for Cloning; Methylation of the cDNA; Linker Kinase Reaction; Test of Ligation Efficiency; Ligation of the Phosphorylated Linkers to the cDNA; EcoRI Digestion and Removal of Excess Linkers from the cDNA; Size Fractionation of the Linkered cDNA; Ligating Fragments into pUC Vectors; Transformation of Host Bacteria; Screening for Recombinant Phages and Plasmids; References; Primer Extension Sequencing of RNA Viruses; Precautions when Handling RNA 327 $aNucleotide Sequencing of Influenza Virus GenesPrimer Extension Sequencing of Influenza A Viruses; Primer Extension Sequencing of Flavivirus Genes; Comments; References; Appendix: Buffers and Reagents; Forensic Applications of DNA Profiling; MuItiIocus Probes; Single Locus Probes; Methods; DNA Profiling in Forensic Casework; References; Application of Nucleic Acid Probes to the Identification of Bacterial Enteric Pathogens; Identification of Enterotoxigenic Escherichia coli from Faecal Specimens; Gene Probes for other Bacterial Enteric Pathogens 327 $aProspects for Nucleic Acid Probes in Clinical Microbiology 330 $aGenetic manipulation is no longer the province of the specialized researcher. It is finding widespread application in all fields of medicine and biology. Nevertheless, application of these relatively new techniques to new areas of research is often fraught with unexpected problems and difficulties. Based on the Society for Applied Bacteriology's Autumn 1989 Conference, this unique volume covers a wide and very up-to-date range of techniques used in genetic engineering. These include the isolation and analysis of DNA and RNA from cells and tissues, the selection and use of phage and plasmic vec 410 0$aTechnical series (Society for Applied Bacteriology) ;$vno. 28. 606 $aGenetic engineering$vCongresses 606 $aBiotechnology$vCongresses 615 0$aGenetic engineering 615 0$aBiotechnology 676 $a575.10724 676 $a660/.65 701 $aGrange$b John M$0968924 701 $aFox$b A$g(Arnold),$f1927-$0968925 701 $aMorgan$b N. L$g(Neil L.),$f1951-$0968926 712 02$aSociety for Applied Bacteriology.$bAutumn Meeting$f(1989 :$eSouth Bank Polytechnic) 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910830959103321 996 $aGenetic manipulation$92201324 997 $aUNINA