LEADER 01140nam--2200361---450- 001 990003379760203316 005 20100316121944.0 010 $a0-19-531025-X 035 $a000337976 035 $aUSA01000337976 035 $a(ALEPH)000337976USA01 035 $a000337976 100 $a20100316d2007----km-y0itay50------ba 101 $aeng 102 $aGB 105 $a||||||||001yy 200 1 $aTerror in the balance$esecurity, liberty, and the courts$fEric A. Posner, Adrian Vermeule 210 $aOxford$cUniversity press$d2007 215 $a319 p.$d24 cm 606 0 $aDiritti di libertà$xEffetti [delle] Misure di sicurezza$yStati Uniti d'America$2BNCF 676 $a342.730412 700 1$aPOSNER,$bEric A.$0254848 701 1$aVERMEULE,$bAdrian$0607222 801 0$aIT$bsalbc$gISBD 912 $a990003379760203316 951 $aXXIV.2.M. 351$b66794 G.$cXXIV.2.M.$d00256651 959 $aBK 969 $aGIU 979 $aCHIARA$b90$c20100316$lUSA01$h1218 979 $aCHIARA$b90$c20100316$lUSA01$h1219 979 $aCHIARA$b90$c20100316$lUSA01$h1219 996 $aTerror in the balance$91127450 997 $aUNISA LEADER 05142nam 22006254a 450 001 9910830870903321 005 20170810174426.0 010 $a1-280-72157-X 010 $a9786610721573 010 $a0-470-07908-8 010 $a0-470-07909-6 035 $a(CKB)2670000000084601 035 $a(EBL)281838 035 $a(OCoLC)184896927 035 $a(SSID)ssj0000174664 035 $a(PQKBManifestationID)11164219 035 $a(PQKBTitleCode)TC0000174664 035 $a(PQKBWorkID)10187832 035 $a(PQKB)10037299 035 $a(MiAaPQ)EBC281838 035 $a(PPN)190654384 035 $a(EXLCZ)992670000000084601 100 $a20060505d2007 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aHPLC, a practical user's guide$b[electronic resource] /$fMarvin C. McMaster 205 $a2nd ed. 210 $aHoboken, N.J. $cWiley-Interscience$dc2007 215 $a1 online resource (254 p.) 300 $aDescription based upon print version of record. 311 $a0-471-75401-3 320 $aIncludes bibliographical references (p. 233-234) and index. 327 $aHPLC; CONTENTS; PREFACE; I HPLC PRIMER; 1 Advantages and Disadvantages of HPLC; 1.1 How It Works; 1.1.1 A Separation Model of the Column; 1.1.2 Basic Hardware: A Quick, First Look; 1.1.3 Use of Solvent Gradients; 1.1.4 Ranges of Compounds; 1.2 Other Ways to Make My Separation; 1.2.1 FPLC-Fast Protein Liquid Chromatography; 1.2.2 LC-Traditional Liquid Chromatography; 1.2.3 GLC-Gas Liquid Chromatography; 1.2.4 SFC-Supercritical Fluid Chromatography; 1.2.5 TLC-Thin Layer Chromatography; 1.2.6 EP-Electrophoresis; 1.2.7 CZE-Capillary Zone Electrophoresis; 2 Selecting an HPLC System 327 $a2.1 Characteristic Systems2.1.1 Finding a Fit: Detectors and Data Processing; 2.1.2 System Models: Gradient Versus Isocratic; 2.1.3 Vendor Selection; 2.1.4 Brand Names and Clones; 2.1.5 Hardware-Service-Support; 2.2 System Cost Estimates; 2.2.1 Type I System-QC Isocratic (Cost: 10-15,000); 2.2.2 Type II System-Research Gradient (Cost: 20-25,000); 2.2.3 Type III System-Automated Clinical (Cost: 25-35,000); 2.2.4 Type IV System-Automated Methods (Cost: 30-50,000); 2.3 Columns; 2.3.1 Sizes: Analytical and Preparative; 2.3.2 Separating Modes: Selecting Only What You Need 327 $a2.3.3 Tips on Column Use3 Running Your Chromatograph; 3.1 Set-up and Start-up; 3.1.1 Hardware Plumbing 101: Tubing and Fittings; 3.1.2 Connecting Components; 3.1.3 Solvent Clean-up; 3.1.4 Water Purity Test; 3.1.5 Start-up System Flushing; 3.1.6 Column Preparation and Equilibration; 3.2 Sample Preparation and Column Calibration; 3.2.1 Sample Clean-up; 3.2.2 Plate Counts; 3.3 Your First Chromatogram; 3.3.1 Reproducible Injection Techniques; 3.3.2 Simple Scouting for a Mobile Phase; 3.3.3 Examining the Chromatogram; 3.3.4 Basic Calculations of Results; II HPLC OPTIMIZATION; 4 Separation Models 327 $a4.1 Partition4.1.1 Separation Parameters; 4.1.2 Efficiency Factor; 4.1.3 Separation (Chemistry) Factor; 4.2 Ion Exchange Chromatography; 4.3 Size Exclusion Chromatography; 4.4 Affinity Chromatography; 5 Column Preparation; 5.1 Column Variations; 5.2 Packing Materials and Hardware; 5.3 Column Selection; 6 Column Aging, Diagnosis, and Healing; 6.1 Packing Degrading-Bonded-Phase Loss; 6.2 Dissolved Packing Material-End Voids; 6.3 Bound Material; 6.4 Pressure Increases; 6.5 Column Channeling-Center-Voids; 6.6 Normal Phase, Ion Exchange, and Size Columns; 6.7 Zirconium and Polymer Columns 327 $a7 Partition Chromatography Modifications7.1 Reverse-Phase and Hybrid Silica; 7.1.1 Ionization Suppression; 7.1.2 Ion Pairing; 7.1.3 Organic Modifiers; 7.1.4 Chelation; 7.2 Acidic Phase Silica; 7.3 Reverse-Phase Zirconium; 7.4 Partition Mode Selection; 8 "Nonpartition" Chromatography; 8.1 Ion Exchange; 8.1.1 Cationic:Weak and Strong; 8.1.2 Anionic:Weak and Strong; 8.2 Size Exclusion; 8.2.1 Organic Soluble Samples; 8.2.2 Hydrophilic Protein Separation; 8.3 Affinity Chromatography; 8.3.1 Column Packing Modification; 8.3.2 Chelation and Optically Active Columns; 9 Hardware Specifics 327 $a9.1 System Protection 330 $aThis Second Edition of the classic handbook details how to set up an HPLC system that capitalizes on the latest innovations. It covers new techniques in high-temperature, micro-flow, and ultra-fast chromatography, the linking of an HPLC to a mass spectrometer, and more. Complete with a CD-ROM and appendices, this guide has everything chromatographers need to know to confidently separate, identify, purify, and quantify compounds. Note: CD-ROM/DVD and other supplementary materials are not included as part of eBook file. 606 $aHigh performance liquid chromatography 615 0$aHigh performance liquid chromatography. 676 $a543.84 676 $a543/.84 676 $a615.1901 700 $aMcMaster$b Marvin C$091640 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910830870903321 996 $aHPLC$9397336 997 $aUNINA