LEADER 04024nam 2200601Ia 450 001 9910830780803321 005 20230721021402.0 010 $a1-282-11371-2 010 $a9786612113710 010 $a0-470-45492-X 010 $a0-470-45491-1 035 $a(CKB)1000000000754320 035 $a(EBL)427574 035 $a(OCoLC)476269097 035 $a(SSID)ssj0000145947 035 $a(PQKBManifestationID)11148400 035 $a(PQKBTitleCode)TC0000145947 035 $a(PQKBWorkID)10181994 035 $a(PQKB)11059040 035 $a(MiAaPQ)EBC427574 035 $a(EXLCZ)991000000000754320 100 $a20090129d2009 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aEmerging technology platforms for stem cells$b[electronic resource] /$fedited by Uma Lakshmipathy, Jonathan D. Chesnut, Bhaskar Thyagarajan 210 $aHoboken, N.J. $cJohn Wiley & Sons$dc2009 215 $a1 online resource (550 p.) 300 $aDescription based upon print version of record. 311 $a0-470-14693-1 320 $aIncludes bibliographical references and index. 327 $aEMERGING TECHNOLOGY PLATFORMS FOR STEM CELLS; CONTENTS; Foreword; Preface; Contributors; PART I; 1 Derivation Methods for Human Embryonic Stem Cells: Past, Present, and Future; 2 Isolation of Human Embryonic Stem Cells from Various Stages of the Human Embryo; 3 Derivation of Stem Cells from Epiblasts; 4 Derivation of Embryonic Stem Cells from Parthenogenetic Eggs; 5 Reprogramming Developmental Potential; PART II; 6 Adult Stem Cells and Their Role in Endogenous Tissue Repair; 7 Greater Differentiation Potential of Adult Stem Cells; 8 Cancer Stem Cells 327 $a9 Large-Scale Production of Adult Stem Cells for Clinical UsePART III; 10 Genetic and Epigenetic Features of Stem Cells; 11 Directed Differentiation of Embryonic Stem cells; 12 Identification of Signaling Pathways Involved During Differentiation; PART IV; 13 Media and Extra Cellular Matrix Requirements for Large-Scale ESC Growth; 14 Automated Method for Embryonic Stem Cell Culture; 15 Quantitative 2D Imaging of Human Embryonic Stem Cells; 16 Nanobiotechnology for Stem Cell Culture and Maintenance; 17 Engineering Microenvironments to Control Stem Cell Functions; PART V 327 $a18 Improved Lentiviral Gene Delivery Tools for Stem Cells19 Sleeping Beauty-Mediated Transposition in Stem Cells; 20 PhiC31 Integrase for Modification of Stem Cells; 21 Cell Engineering Using Integrase and Recombinase Systems; PART VI; 22 Human Embryonic Stem Cell-Derived Cardiomyocytes for Cell Therapy and Drug Discovery; 23 Human Embryonic Stem Cells in Drug Discovery; 24 Characterization and Culturing of Adipose-Derived Precursor Cells; 25 Bringing Mesenchymal Stem Cells into the Clinic; Index 330 $aThis book focuses on practical applications for using adult and embryonic stem cells in the pharmaceutical development process. It emphasizes new technologies to help overcome the bottlenecks in developing stem cells as therapeutic agents. A key reference for professionals working in stem cell science, it presents the general principles and methodologies in stem cell research and covers topics such as derivitization and characterization of stem cells, stem cell culture and maintenance, stem cell engineering, applications of high-throughput screening, and stem cell genetic modification with the 606 $aStem cells$xTherapeutic use 606 $aAnimal cell biotechnology 615 0$aStem cells$xTherapeutic use. 615 0$aAnimal cell biotechnology. 676 $a616.02774 676 $a616/.02774 701 $aChesnut$b Jonathan D$01691438 701 $aThyagarajan$b Bhaskar$01596110 701 $aLakshmipathy$b Uma$01596109 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910830780803321 996 $aEmerging technology platforms for stem cells$94067777 997 $aUNINA