LEADER 00885nam a2200265 i 4500 001 991002278009707536 005 20020508193515.0 008 940218s1989 fr ||| | fre 020 $a270180048X 035 $ab10985542-39ule_inst 035 $aPARLA158635$9ExL 040 $aDip.to Filol. Class. e Med.$bita 082 0 $a520 100 1 $aLe Boeuffle, André$0391801 245 13$aLe ciel des Romains /$cAndré Le Boeuffle 260 $aParis :$bDe Boccard,$c1989 300 $a163 p., [4] p. di tav. ;$c24 cm. 490 0 $aAntiques 650 4$aAstronomia$zRoma antica 907 $a.b10985542$b23-02-17$c28-06-02 912 $a991002278009707536 945 $aLE007 S 4454$g1$i2007000008232$lle007$o-$pE0.00$q-$rl$s- $t0$u0$v0$w0$x0$y.i11098880$z28-06-02 996 $aCiel des romains$9746296 997 $aUNISALENTO 998 $ale007$b01-01-94$cm$da $e-$ffre$gfr $h3$i1 LEADER 05420nam 2200649Ia 450 001 9910830771703321 005 20200327181921.0 010 $a1-280-72366-1 010 $a9786610723669 010 $a3-527-60932-6 010 $a3-527-60936-9 035 $a(CKB)1000000000376117 035 $a(EBL)481390 035 $a(SSID)ssj0000171122 035 $a(PQKBManifestationID)11168132 035 $a(PQKBTitleCode)TC0000171122 035 $a(PQKBWorkID)10238020 035 $a(PQKB)10200160 035 $a(MiAaPQ)EBC481390 035 $a(OCoLC)159940039 035 $a(EXLCZ)991000000000376117 100 $a20051013d2006 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aHigh-throughput screening in drug discovery$b[e-book] /$fedited by Jo?rg Hu?ser 210 $aWeinheim $cWiley-VCH ;$a[Chichester $cJohn Wiley, distributor]$dc2006 215 $a1 online resource (371 p.) 225 1 $aMethods and principles in medicinal chemistry ;$vv. 35 300 $aDescription based upon print version of record. 311 $a3-527-31283-8 320 $aIncludes bibliographical references and index. 327 $aHigh-Throughput Screening in Drug Discovery; Foreword; List of Contents; Preface; List of Contributors; Part I Concept of Screening; 1 Chemical Genetics: Use of High-throughput Screening to Identify Small-molecule Modulators of Proteins Involved in Cellular Pathways with the Aim of Uncovering Protein Function; 1.1 Introduction; 1.2 Classical and Chemical Genetics; 1.2.1 Forward and Reverse Screens; 1.3 Identifying Bioactive Molecules; 1.4 Target Identification; 1.4.1 Hypothesis-driven Target Identification; 1.4.2 Affinity-based Target Identification 327 $a1.4.3 Genomic Methods of Target Identification1.4.4 Proteomic Methods; 1.5 Discovery for Basic Research Versus Pharmacotherapy Goals; 1.6 Chemical Genetic Screens in the Academic Setting; 1.7 Conclusions; 2 High-throughput Screening for Targeted Lead Discovery; 2.1 Chemical Libraries for High-throughput Screening; 2.2 Properties of Lead Structures; 2.3 Challenges to High-throughput Screening; 2.4 Assay Technologies for High-throughput Screening; 2.5 Laboratory Automation; 2.6 From Target Selection to Confirmed Hits - the HTS Workflow and its Vocabulary 327 $a2.7 Separating Specific Modulators from Off-Target Effects2.8 Data Analysis and Screening Results; 2.9 Conclusions; Part II Automation Technologies; 3 Tools and Technologies that Facilitate Automated Screening; 3.1 Introduction - the Necessity to Automate; 3.1.1 Compound Libraries; 3.1.2 Targets and Data Points; 3.1.3 Main Issues Facing HTS Groups Today; 3.1.4 Benefits of Miniaturization; 3.1.5 Benefits of Automated HTS; 3.1.6 Screening Strategies; 3.1.7 Ultra HTS (UHTS); 3.2 Sample Carriers; 3.2.1 A Brief History of the Microplate; 3.2.2 Microplate Usage Today; 3.2.3 Microplate Arrays 327 $a3.2.4 Non-microplate Alternatives3.2.4.1 Labchips; 3.2.4.2 LabCDs; 3.2.4.3 LabBrick; 3.2.4.4 Arrayed Compound Screening; 3.3 Liquid Handling Tools; 3.3.1 Main Microplate Dispense Mechanisms; 3.3.1.1 Pin Tools; 3.3.1.2 Air and Positive Displacement; 3.3.1.3 Peristaltic; 3.3.1.4 Solenoid-syringe; 3.3.1.5 Solenoid-pressure bottle; 3.3.1.6 Capillary Sipper; 3.3.1.7 Piezoelectric; 3.3.1.8 Acoustic Transducer; 3.3.2 HTS Liquid Handling Applications and Dispensing Technologies Used; 3.3.2.1 Bulk Reagent and Cell Addition; 3.3.2.2 Compound Reformatting and Nanoliter Dispensing 327 $a3.3.2.3 Cherry Picking and Serial Dilution3.3.2.4 Microplate Washing; 3.4 Detection Technologies; 3.4.1 Main Detection Modalities Used in HTS; 3.4.2 Plate Readers; 3.4.3 Plate Imagers; 3.4.3.1 Macro-imaging; 3.4.3.2 Micro-imaging; 3.4.4 Dispense and Read Devices; 3.4.5 Other Detection Technologies; 3.4.6 Automation of Detection Technologies; 3.4.7 Potential Sources of Reading Error; 3.5 Laboratory Robotics; 3.5.1 Traditional Workstations; 3.5.2 Robotic Sample Processors; 3.5.3 Plate Storage Devices; 3.5.4 Plate Moving Devices; 3.5.5 Fully Integrated Robotic Systems; 3.5.6 Turnkey Workstations 327 $a3.5.7 Automated Cell Culture Systems 330 $aBacked by leading authorities, this is a professional guide to successful compound screening in pharmaceutical research and chemical biology, including the chemoinformatic tools needed for correct data evaluation. Chapter authors from leading pharmaceutical companies as well as from Harvard University discuss such factors as chemical genetics, binding, cell-based and biochemical assays, the efficient use of compound libraries and data mining using cell-based assay results.For both academics and professionals in the pharma and biotech industries working on small molecule screening. 410 0$aMethods and principles in medicinal chemistry ;$vv. 35. 606 $aHigh throughput screening (Drug development) 606 $aPharmaceutical chemistry 615 0$aHigh throughput screening (Drug development) 615 0$aPharmaceutical chemistry. 676 $a615.19 676 $a615.1900285 686 $a44.38$2bcl 701 $aHu?ser$b Jo?rg$01663656 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910830771703321 996 $aHigh-throughput screening in drug discovery$94021126 997 $aUNINA