LEADER 05530nam 2200721Ia 450 001 9910830737903321 005 20230721030637.0 010 $a1-281-28497-1 010 $a9786611284978 010 $a0-470-27626-6 010 $a0-470-27625-8 035 $a(CKB)1000000000400795 035 $a(EBL)335762 035 $a(OCoLC)214281848 035 $a(SSID)ssj0000206423 035 $a(PQKBManifestationID)11206821 035 $a(PQKBTitleCode)TC0000206423 035 $a(PQKBWorkID)10215079 035 $a(PQKB)10169988 035 $a(MiAaPQ)EBC335762 035 $a(EXLCZ)991000000000400795 100 $a20071004d2008 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aMultidimensional liquid chromatography$b[electronic resource] $etheory and applications in industrial chemistry and the life sciences /$fedited by Steven A. Cohen, Mark R. Schure 210 $aHoboken, N.J. $cWiley-Interscience$dc2008 215 $a1 online resource (490 p.) 300 $aDescription based upon print version of record. 311 $a0-471-73847-6 320 $aIncludes bibliographical references and index. 327 $aMULTIDIMENSIONAL LIQUID CHROMATOGRAPHY; CONTENTS; Foreword; Preface; Contributors; 1 Introduction; 1.1 Previous Literature Which Covers MDLC; 1.2 How this Book is Organized; References; PART I THEORY; 2 Elements of the Theory of Multidimensional Liquid Chromatography; 2.1 Introduction; 2.2 Peak Capacity; 2.3 Resolution; 2.4 Orthogonality; 2.5 Two-Dimensional Theory of Peak Overlap; 2.6 Dimensionality, Peak Ordering, and Clustering; 2.7 Theory of Zone Sampling; 2.8 Dilution and Limit of Detection; 2.9 Chemometric Analysis; 2.10 Future Directions; References 327 $a3 Peak Capacity in Two-Dimensional Liquid Chromatography3.1 Introduction; 3.2 Theory; 3.3 Procedures; 3.4 Results and Discussion; 3.5 Conclusions; Appendix 3A Generation of Random Correlated Coordinates; Appendix 3B Derivation of Limiting Correlation Coefficient r; References; 4 Decoding Complex 2D Separations; 4.1 Introduction; 4.2 Fundamentals: The Statistical Description of Complex Multicomponent Separations; 4.3 Decoding 1D and 2D Multicomponent Separations by Using the SMO Poisson Statistics; 4.4 Decoding Multicomponent Separations by the Autocovariance Function 327 $a4.5 Application to 2D Separations4.5.1 Results from SMO Method; 4.5.2 Results from 2D Autocovariance Function Method; 4.6 Concluding Remarks; Acknowledgments; References; PART II COLUMNS, INSTRUMENTATION AND METHODS DEVELOPMENT; 5 Instrumentation for Comprehensive Multidimensional Liquid Chromatography; 5.1 Introduction; 5.2 Heart-Cutting Versus Comprehensive Mode; 5.3 Chromatographic Hardware; 5.3.1 Valves; 5.4 CE Interfaces; 5.4.1 Gated Interface for HPLC-CE; 5.4.2 Microfluidic Valves for On-Chip Multidimensional Analysis; 5.5 Columns and Combinations 327 $a5.5.1 Column Systems, Dilution, and Splitting5.6 Detection; 5.7 Computer Hardware and Software; 5.7.1 Software Development; 5.7.2 Valve Sequencing; 5.7.3 Data Format and Storage; 5.8 Zone Visualization; 5.8.1 Contour Visualization; 5.8.2 2D Peak Presentation; 5.8.3 Zone Visualization in Specific Chemical (pI) Regions; 5.8.4 External Plotting Programs; 5.8.5 Difference Plots; 5.8.6 Multi-channel Data; 5.9 Data Analysis and Signal Processing; 5.10 Future Prospects; References; 6 Method Development in Comprehensive Multidimensional Liquid Chromatography; 6.1 Introduction; 6.2 Previous Work 327 $a6.3 Column Variables6.4 Method Development; 6.4.1 The Cardinal Rules of 2DLC Method Development; 6.5 Planning the Experiment; 6.6 General Comments on Optimizing the 2DLC Experiment: Speed-Resolution Trade-off; Acknowledgment; References; 7 Monolithic Columns and Their 2D-HPLC Applications; 7.1 Introduction; 7.2 Monolithic Polymer Columns; 7.2.1 Structural Properties of Polymer Monoliths; 7.2.2 Chromatographic Properties of Polymer Monolithic Columns; 7.2.3 Two-Dimensional HPLC Using Polymer Monoliths; 7.3 Monolithic Silica Columns; 7.3.1 Preparation 327 $a7.3.2 Structural Properties of Monolithic Silica Columns 330 $aMultidimensional Liquid Chromatography (MDLC) is a very powerful separation technique for analyzing exceptionally complex samples in one step. This authoritative reference presents a number of recent contributions that help define the current art and science of MDLC. Topics covered include instrumentation, theory, methods development, and applications of MDLC in the life sciences and in industrial chemistry. With the information to help you perform very difficult separations of complex samples, this reference includes chapters contributed by leading experts or teams of experts. 606 $aBiochemistry 606 $aChemical engineering 606 $aChemistry, Technical 606 $aLiquid chromatography 615 0$aBiochemistry. 615 0$aChemical engineering. 615 0$aChemistry, Technical. 615 0$aLiquid chromatography. 676 $a543.84 676 $a543/.84 686 $a35.71$2bcl 686 $a35.29$2bcl 686 $a58.14$2bcl 686 $aVG 7500$2rvk 701 $aCohen$b Steven A.$f1953-$01593282 701 $aSchure$b Mark R$01593283 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910830737903321 996 $aMultidimensional liquid chromatography$93913342 997 $aUNINA