LEADER 01546nam--2200421---450- 001 990000399070203316 005 20100830100944.0 010 $a88-243-1380-9 035 $a0039907 035 $aUSA010039907 035 $a(ALEPH)000039907USA01 035 $a0039907 100 $a20010411d2001----km-y0itay0103----ba 101 0 $aita 102 $aIT 105 $a||||||||001yy 200 1 $a<> procedimento di controllo degli aiuti pubblici alle imprese$etra ordinamento comunitario e ordinamento interno$fOrnella Porchia 210 $aNapoli$cJovene$d2001 215 $a323 p.$d24 cm 225 2 $aMemorie del dipartimento di scienze giuridiche$i5. ser.$v11 410 0$12001$aMemorie del Dipartimento di scienze giuridiche$fUniversitą di Torino$hser. 5.$v10$i5. ser. 606 $aImprese$xFinanziamenti pubblici 606 $aComunitą economica europea$xPolitica industriale 676 $a338.4300094 700 1$aPORCHIA,$bOrnella$0263065 801 0$aIT$bsalbc$gISBD 912 $a990000399070203316 951 $aXXX.A. Coll. 122/ 33 (X 24 XXV 11)$b28969 G.$cXXX.A. Coll. 122/ 33 (X 24)$d00072902 959 $aBK 969 $aGIU 979 $aCHIARA$b40$c20010411$lUSA01$h1156 979 $aCHIARA$b40$c20010411$lUSA01$h1158 979 $c20020403$lUSA01$h1648 979 $aPATRY$b90$c20040406$lUSA01$h1628 979 $aFIORELLA$b90$c20070518$lUSA01$h0803 979 $aRSIAV4$b90$c20100830$lUSA01$h1009 996 $aProcedimento di controllo degli aiuti pubblici alle imprese$9874193 997 $aUNISA LEADER 05405nam 2200685 450 001 9910830618803321 005 20230721033028.0 010 $a1-281-94680-X 010 $a9786611946807 010 $a3-527-62229-2 010 $a3-527-62230-6 035 $a(CKB)1000000000549420 035 $a(EBL)481336 035 $a(SSID)ssj0000229680 035 $a(PQKBManifestationID)11174950 035 $a(PQKBTitleCode)TC0000229680 035 $a(PQKBWorkID)10173121 035 $a(PQKB)10369351 035 $a(MiAaPQ)EBC481336 035 $a(OCoLC)264389562 035 $a(EXLCZ)991000000000549420 100 $a20160819h20082008 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aProteomics in practice $ea guide to successful experimental design /$fedited by Reiner Westermeier, Tom Naven, Hans-Rudolf Ho?pker 205 $aSecond, completely revised edition. 210 1$aWeinheim, Germany :$cWILEY-VCH Verlag GmbH & Co. KGaA,$d2008. 210 4$d©2008 215 $a1 online resource (504 p.) 300 $aDescription based upon print version of record. 311 $a3-527-31941-7 320 $aIncludes bibliographical references and index. 327 $aProteomics in Practice; Contents; Preface; Foreword; Abbreviations, Symbols, Units; Introduction; 1 History; 2 Critical Points; 2.1 Challenges of the Protein Samples; 2.2 Challenges of the Analysis Systems; 3 Proteomics Strategies; 3.1 Proteome Mapping; 3.2 Differential Analysis; 3.3 Time Point Experiments; 3.4 Verification of Targets or Biomarkers; 3.5 Integration of Results into Biological Context; 3.6 Systems Biology; 4 Concept of Experimental Planning; 4.1 Biological Replicates; 4.2 Pooling of Samples: Yes or No?; 4.3 Pre-fractionation of Samples: Yes or No? 327 $a4.4 Which is the Best Workflow to Start With?Part I: Proteomics Technology; 1 Electrophoretic Techniques; 1.1 The Principle of Electrophoresis and Some Methodological Background; 1.1.1 Free Flow Electrophoretic Methods; 1.1.2 Gels for Electrophoretic Techniques; 1.1.3 Electroendosmosis Effects; 1.2 Polyacrylamide Gel Electrophoresis; 1.2.1 The Polyacrylamide Gel; 1.2.2 SDS Polyacrylamide Gel Electrophoresis; 1.2.3 Blue Native Electrophoresis; 1.2.4 Cationic Detergent Electrophoresis; 1.3 Blotting; 1.3.1 Electrophoretic Transfer; 1.3.2 Protein Detection on the Membrane 327 $a1.4 Isoelectric Focusing1.4.1 Theoretical Background; 1.4.2 Preparation of IEF Gels; 1.4.3 Isoelectric Focusing in Proteomics; 1.5 Two-dimensional Electrophoresis; 1.5.1 Sample Preparation; 1.5.2 Pre-labeling of Proteins for Difference Gel Electrophoresis; 1.5.3 First Dimension: Isoelectric Focusing in IPG Strips; 1.5.4 Second Dimension: SDS Electrophoresis; 1.5.5 Detection of Protein Spots; 1.6 Image Analysis; 1.6.1 Image Acquisition; 1.6.2 Image Analysis and Evaluation; 1.6.3 Use of 2-D Electrophoresis Data; 1.7 Spot Handling; 1.7.1 Spot Picking; 1.7.2 Protein Cleavage 327 $a2 Liquid Chromatography Techniques2.1 Basic Principles of Important Liquid Chromatography Techniques; 2.1.1 Ion Exchange Chromatography; 2.1.2 Reversed Phase Chromatography; 2.1.3 Affinity Chromatography; 2.1.4 Gel Filtration; 2.2 Strategic Approach and General Applicability; 2.3 Liquid Chromatography Techniques and Applications in Proteome Analysis; 2.3.1 Peptide Separation; 2.3.2 2DLC Peptide Separation; 2.3.3 Affinity Chromatography and LC-MS/MS; 2.3.4 Protein Pre-fractionation; 2.4 Practical Considerations and Application of LC-based Protein Pre-fractionation 327 $a2.4.1 Sample Extraction and Preparation2.4.2 Experimental Setup; 2.4.3 Ion Exchange Chromatography and Protein Pre-fractionation; 2.4.4 Reversed Phase Chromatography and Protein Pre-fractionation; 2.4.5 Fraction Size and Number of Fractions; 2.5 Critical Review and Outlook; 3 Mass Spectrometry; 3.1 Ionization; 3.1.1 Matrix Assisted Laser Desorption Ionization; 3.1.2 Electrospray Ionization; 3.2 Ion Separation; 3.2.1 Time-of-Flight Analyzer; 3.2.2 Triple Quadrupole Analyzer; 3.2.3 Quadrupole Ion Trap; 3.2.4 Quadrupole Time-of-Flight; 3.2.5 Hybrid Triple Quadrupole Linear Ion Trap 327 $a3.2.6 TOF/TOF Analyzer 330 $aStill the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. 606 $aProteomics$vHandbooks, manuals, etc 606 $aProteins$xAnalysis 606 $aMolecular biology 615 0$aProteomics 615 0$aProteins$xAnalysis. 615 0$aMolecular biology. 676 $a572.6 676 $a572.64 702 $aWestermeier$b Reiner 702 $aNaven$b Tom 702 $aHo?pker$b Hans-Rudolf 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910830618803321 996 $aProteomics in practice$94107079 997 $aUNINA