LEADER 05444nam 2200721Ia 450 001 9910830570303321 005 20230617021305.0 010 $a1-280-52027-2 010 $a9786610520275 010 $a3-527-60518-5 010 $a3-527-60266-6 035 $a(CKB)1000000000019310 035 $a(EBL)481319 035 $a(SSID)ssj0000204788 035 $a(PQKBManifestationID)11172538 035 $a(PQKBTitleCode)TC0000204788 035 $a(PQKBWorkID)10191420 035 $a(PQKB)11550447 035 $a(MiAaPQ)EBC481319 035 $a(OCoLC)85820425 035 $a(EXLCZ)991000000000019310 100 $a20030725d2004 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aMolecular biology in medicinal chemistry$b[electronic resource] /$fedited by Th. Dingermann, D. Steinhilber and G. Folkers 210 $aWeinheim $cWiley-VCH$d2004 215 $a1 online resource (437 p.) 225 1 $aMethods and principles in medicinal chemistry ;$vv. 21 300 $aDescription based upon print version of record. 311 $a3-527-30431-2 320 $aIncludes bibliographical references and index. 327 $aMolecular Biology in Medicinal Chemistry; Contents; Preface; Foreword; Contributors; Part I Molecular Targets; 1 Cellular Assays in Drug Discovery; 1.1 Introduction; 1.1.1 Positioning Cellular Assays; 1.1.2 Impact on Drug Discovery; 1.1.3 Classification of Cellular Assays; 1.1.4 Progress in Tools and Technologies for Cellular Compound Profiling; 1.2 Membrane Proteins and Fast Cellular Responses; 1.2.1 Receptors; 1.2.1.1 FLIPR Technology for Detection of Intracellular Calcium Release; 1.2.1.2 Competitive Immunoassay for Detection of Intracellular cAMP 327 $a1.2.1.3 Enzyme Fragment Complementation (EFC) Technology1.2.2 Membrane Transport Proteins; 1.2.2.1 Ion Channels; 1.2.2.2 MDR Proteins; 1.3 Gene and Protein Expression Profiling in High-throughput Formats; 1.3.1 Reporter Gene Assays in Lead Finding; 1.3.2 Reporter Gene Assays in Lead Optimization; 1.4 Spatio-temporal Assays and Subpopulation Analysis; 1.4.1 Phosphorylation Stage-specific Antibodies; 1.4.2 Target-protein-specific Antibodies; 1.4.3 Protein-GFP Fusions; 1.4.4 Fluorescence Resonance Energy Transfer (FRET) 327 $a1.4.5 GPCR Activation using Bioluminescence Resonance Energy Transfer (BRET)1.4.6 Protein Fragment Complementation Assays (PCA); 1.5 Phenotypic Assays; 1.5.1 Proliferation/Respiration/Toxicity; 1.5.2 Apoptosis; 1.5.3 Differentiation; 1.5.4 Monitoring Cell Metabolism; 1.5.5 Other Phenotypic Assays; Acknowledgments; References; 2 Gene Knockout Models; 2.1 Introduction; 2.2 Gene Knockout Mice; 2.2.1 ES Cells; 2.2.2 Targeting Vector; 2.2.3 Selection of Recombinant ES Cells; 2.2.4 Injection of Recombinant ES Cells into Blastocysts and Blastocyst Transfer to Pseudopregnant Recipients 327 $a2.2.5 Chimeras and F1 and F2 Offspring2.3 Tissue-Specific Gene Expression; 2.3.1 Ligand-Activated CRE Recombinases; 2.3.2 The Tetracycline/Doxycycline-Inducible Expression System; 2.4 Transgenic Mice; 2.5 Targeted Gene Disruption in Drosophila; 2.6 Targeted Gene Knockdown in Zebrafish; 2.7 Targeted Caenorhabditis Elegans Deletion Strains; References; 3 Reporter Gene Assay Systems for the Investigation of G-protein-coupled Receptors; 3.1 Receptors and Cellular Communication; 3.1.1 Ion Channel-linked Receptors; 3.1.2 Enzyme-linked Cell-surface Receptors; 3.1.3 GPCRs 327 $a3.2 Affinity and Activity of GPCR Ligands3.3 The Role of Transcription Factors in Gene Expression; 3.3.1 CREB; 3.3.2 SRF; 3.3.3 STAT Proteins; 3.3.4 c-Jun; 3.3.5 NF-AT; 3.4 Reporter Genes; 3.4.1 CAT; 3.4.2 ?-Gal; 3.4.3 ?-Glucuronidase; 3.4.4 AP; 3.4.5 SEAP; 3.4.6 ?-Lactamase; 3.4.7 Luciferase; 3.4.8 GFP; 3.5 Reporter Gene Assay Systems for the Investigation of GPCRs; 3.5.1 Application of Luciferase as a Reporter Gene; 3.5.2 Application of other Reporter Genes for the Investigation of GPCRs; References; 4 From the Human Genome to New Drugs: The Potential of Orphan G-protein-coupled Receptors 327 $a4.1 Introduction 330 $aThis readily comprehensible book explains the identification of molecular targets via cellular assays, reporter genes or transgenic models, as well as surveying recent advances in the synthesis, separation and analysis of drugs. A special section is devoted to molecular genetics methods.With its examination of these novel methods and generous practical advice, this is essential reading for all pharmaceutical chemists, molecular biologists and medical researchers using molecular methods to study drugs and their action. 410 0$aMethods and principles in medicinal chemistry ;$vv. 21. 606 $aChiral drugs 606 $aGenomics 606 $aMolecular biology 606 $aMolecular pharmacology 606 $aPharmacogenomics 615 0$aChiral drugs. 615 0$aGenomics. 615 0$aMolecular biology. 615 0$aMolecular pharmacology. 615 0$aPharmacogenomics. 676 $a615.19 701 $aDingermann$b Theodor$01345340 701 $aFolkers$b Gerd$0505529 701 $aSteinhilber$b Dieter$f1959-$01701529 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910830570303321 996 $aMolecular biology in medicinal chemistry$94085321 997 $aUNINA