LEADER 05633nam 2200685Ia 450 001 9910830521203321 005 20170810181519.0 010 $a1-118-10272-X 010 $a1-283-02506-X 010 $a9786613025067 010 $a0-470-87797-9 010 $a0-470-87796-0 035 $a(CKB)2560000000058442 035 $a(EBL)698753 035 $a(OCoLC)705354454 035 $a(SSID)ssj0000470924 035 $a(PQKBManifestationID)11297619 035 $a(PQKBTitleCode)TC0000470924 035 $a(PQKBWorkID)10415722 035 $a(PQKB)11347064 035 $a(MiAaPQ)EBC698753 035 $a(PPN)159210933 035 $a(EXLCZ)992560000000058442 100 $a20100331d2011 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aLight scattering, size exclusion chromatography, and asymmetric flow field flow fractionation$b[electronic resource] $epowerful tools for the characterization of polymers, proteins, and nanoparticles /$fStepan Podzimek 210 $aHoboken, NJ $cWiley$dc2011 215 $a1 online resource (373 p.) 300 $aDescription based upon print version of record. 311 $a0-470-38617-7 320 $aIncludes bibliographical references and index. 327 $aLight Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation; Contents; Preface; 1 Polymers; 1.1 Introduction; 1.2 Molecular Structure of Polymers; 1.2.1 Macromolecules in Dilute Solution; 1.3 Molar Mass Distribution; 1.3.1 Description of Molar Mass Distribution; 1.3.1.1 Distribution Functions; 1.3.1.2 Molar Mass Averages; 1.4 Methods for the Determination of Molar Mass; 1.4.1 Method of End Groups; 1.4.2 Osmometry; 1.4.2.1 Vapor Pressure Osmometry; 1.4.2.2 Membrane Osmometry; 1.4.3 Dilute Solution Viscometry; 1.4.3.1 Properties of Mark-Houwink Exponent 327 $a1.4.3.2 Molecular Size from Intrinsic Viscosity1.4.3.3 Dependence of Intrinsic Viscosity on Polymer Structure, Temperature, and Solvent; 1.4.4 Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry; 1.4.5 Analytical Ultracentrifugation; 1.5 Keynotes; 1.6 References; 2 Light Scattering; 2.1 Theory and Basic Principles; 2.2 Types of Light Scattering; 2.2.1 Static Light Scattering; 2.2.1.1 Particle Scattering Functions; 2.2.1.2 Light Scattering Formalisms; 2.2.1.3 Processing the Experimental Data; 2.2.2 Dynamic Light Scattering; 2.3 Light Scattering Instrumentation 327 $a2.4 Specific Refractive Index Increment2.5 Light Scattering in Batch and Chromatography Mode; 2.6 Parameters Affecting Accuracy of Molar Mass Determined by Light Scattering; 2.7 Examples of Light Scattering Measurement in Batch Mode; 2.8 Keynotes; 2.9 References; 3 Size Exclusion Chromatography; 3.1 Introduction; 3.2 Separation Mechanisms; 3.2.1 Steric Exclusion; 3.2.2 Restricted Diffusion; 3.2.3 Separation by Flow; 3.2.4 Peak Broadening and Separation Efficiency; 3.2.5 Secondary Separation Mechanisms; 3.3 Instrumentation; 3.3.1 Solvents; 3.3.2 Columns and Column Packing; 3.3.3 Detectors 327 $a3.3.3.1 UV Detector3.3.3.2 Refractive Index Detector; 3.3.3.3 Infrared Detector; 3.3.3.4 Evaporative Light Scattering Detector; 3.3.3.5 Viscosity Detector; 3.3.3.6 Light Scattering Detector; 3.3.3.7 Other Types of Detectors; 3.4 Column Calibration; 3.4.1 Universal Calibration; 3.4.2 Flow Marker; 3.5 SEC Measurements and Data Processing; 3.5.1 Sample Preparation; 3.5.2 Determination of Molar Mass and Molar Mass Distribution; 3.5.3 Reporting Results; 3.5.4 Characterization of Chemical Composition of Copolymers and Polymer Blends; 3.5.5 Characterization of Oligomers 327 $a3.5.6 Influence of Separation Conditions3.5.7 Accuracy, Repeatability, and Reproducibility of SEC Measurements; 3.6 Applications of SEC; 3.7 Keynotes; 3.8 References; 4 Combination of SEC and Light Scattering; 4.1 Introduction; 4.2 Data Collection and Processing; 4.2.1 Processing MALS Data; 4.2.1.1 Debye Fit Method; 4.2.1.2 Zimm Fit Method; 4.2.1.3 Berry Fit Method; 4.2.1.4 Random Coil Fit Method; 4.2.1.5 Influence of Light Scattering Formalism on Molar Mass and RMS Radius; 4.2.2 Determination of Molar Mass and RMS Radius Averages and Distributions; 4.2.3 Chromatogram Processing 327 $a4.2.4 Influence of Concentration and Second Virial Coefficient 330 $aA comprehensive, practical approach to three powerful methods of polymer analysis and characterization This book serves as a complete compendium of three important methods widely used for the characterization of synthetic and natural polymers-light scattering, size exclusion chromatography (SEC), and asymmetric flow field flow fractionation (A4F). Featuring numerous up-to-date examples of experimental results obtained by light scattering, SEC, and A4F measurements, Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation takes an all-in-one approach to 606 $aPolymers$xAnalysis 606 $aPolymers$xSeparation 606 $aLight$xScattering 606 $aChromatographic analysis 615 0$aPolymers$xAnalysis. 615 0$aPolymers$xSeparation. 615 0$aLight$xScattering. 615 0$aChromatographic analysis. 676 $a543/.8 676 $a547.7 700 $aPodzimek$b Stepan$f1955-$01609895 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910830521203321 996 $aLight scattering, size exclusion chromatography, and asymmetric flow field flow fractionation$93937376 997 $aUNINA