LEADER 05428nam 2200673Ia 450 001 9910830451103321 005 20230828230856.0 010 $a1-282-30244-2 010 $a9786612302442 010 $a3-527-61194-0 010 $a3-527-60870-2 035 $a(CKB)1000000000687512 035 $a(EBL)481286 035 $a(OCoLC)468787369 035 $a(SSID)ssj0000343201 035 $a(PQKBManifestationID)11255581 035 $a(PQKBTitleCode)TC0000343201 035 $a(PQKBWorkID)10289226 035 $a(PQKB)10946255 035 $a(MiAaPQ)EBC481286 035 $a(EXLCZ)991000000000687512 100 $a20050629d2006 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aStandards of mouse model phenotyping$b[electronic resource] /$fedited by Martin Hrabede Angelis, Pierre Chambon, and Steve Brown 210 $aWeinheim $cWiley-VCH$dc2006 215 $a1 online resource (359 p.) 300 $aDescription based upon print version of record. 311 $a3-527-31031-2 320 $aIncludes bibliographical references and index. 327 $aStandards of Mouse Model Phenotyping; Foreword; Table of Contents; Preface; 1 Characterizing Hearing in Mice; 1.1 Introduction; 1.2 Behavioral Tests of Hearing; 1.3 Physiological Tests of Hearing; 1.4 Anatomy of the Ear; 1.5 Conclusions; Acknowledgements; 2 Molecular Phenotyping: Gene Expression Profiling; 2.1 Why this Screen? Medical and Biological Relevance; 2.2 Examples: Diseases of Mouse and Man; 2.3 Diagnostic Methods: History and State of the Art; 2.4 Technical Requirements for Screening Protocols (Short): First and Second Line Approaches; 2.5 Logistics (Whom, When, How Many, Why) 327 $a2.5.1 Choice of Platform2.5.2 Biological Samples; 2.6 Trouble Shooting; 2.6.1 Preparation of Hybridization Target; 2.6.2 Critical Issues of Chip Hybridization; 2.6.3 Image Processing and Array Design; 2.7 Short-term Outlook; 3 Screening for Bone and Cartilage Phenotypes in Mice; 3.1 Introduction; 3.1.1 The Skeleton; 3.1.2 Skeletal Development in the Embryo; 3.1.3 Growth and Maintenance of Bone and Cartilage; 3.1.4 Diseases Involving Cartilage and Bone; 3.1.5 The Mouse as a Model for Skeletal Diseases; 3.2 Screening Protocols; 3.2.1 Morphological Analysis; 3.2.1.1 Protocol 327 $a3.2.2 X-Ray Analysis3.2.2.1 General; 3.2.2.2 Imaging; 3.2.2.3 X-Ray Analysis; 3.2.2.4 Protocol; 3.2.3 DXA-Analysis; 3.2.3.1 General; 3.2.3.2 Advantages; 3.2.3.3 Disadvantages; 3.2.3.4 Small Animal Applications; 3.2.3.5 Precision and Accuracy; 3.2.3.6 Considerations; 3.2.3.7 Protocol; 3.2.4 Biochemical Bone Markers; 3.2.4.1 Clinical Utility of Biochemical Markers of Bone Turnover in Small Animals; 3.2.4.2 Mouse Markers of Bone Turnover/Metabolism and Hormonal Regulation; 3.2.4.3 Variability/Sensitivity/Sample Choice; 3.2.4.4 Which Markers Should be Used During the Screen? 327 $a3.2.5 Advanced Small Animal Imaging Techniques3.2.5.1 pQCT; 3.2.5.2 ?CT; 3.2.5.3 ?MRI; 3.2.5.4 Whole-mount Skeletal Preparations; 3.2.5.5 Histomorphometry; 3.2.5.6 Miscellaneous; 3.2.5.7 Order of the Tests; 3.3 Conclusion; List of Abbreviations; Appendix; ?CT Volumetric Data Processing; MRI Principles; 4 Clinical Chemical Screen; 4.1 Introduction; 4.1.1 Relevance of the Screen; 4.1.2 Biology and Medical Application; 4.1.2.1 Biology of Clinical Chemical Parameters; 4.1.2.2 Medical Application; 4.2 Diseases in Mouse and Humans; 4.2.1 Diagnostic Impact of Clinical Chemistry 327 $a4.2.2 Clinical Chemistry in Selected Disorders4.2.2.1 Hypercholesterolemia; 4.2.2.2 Albuminuria; 4.2.2.3 Acute Myeloid Leukemia (AML); 4.3 Clinical Chemistry as Diagnostic Tool; 4.3.1 History; 4.3.2 State of the Art; 4.4 Technical Requirements and Screening Protocols; 4.4.1 Technical Requirements; 4.4.1.1 Blood Collection; 4.4.1.2 Sample Preparation; 4.4.1.3 Sample Analysis; 4.4.2 Screening Protocols; 4.4.2.1 Primary Screen; 4.4.2.2 Secondary Screen; 4.4.2.3 Tertiary Screen; 4.5 Logistics of the Screen; 4.5.1 General Considerations; 4.5.2 Lessons from ENU Mutants; 4.6 Trouble Shooting 327 $a4.6.1 Factors Interfering In Vivo 330 $aThis is the first book in the field of mouse genetics to provide comprehensive and standardized methods for the characterization of laboratory mice. The editor is Director of the German Mouse Clinic and member of the Project Committee of the German National Genome Research Network and provides here a brief introduction to the mouse as a model for diseases and functional analysis of genes and proteins. Throughout, he focuses on the characterization of mouse models using the latest phenotyping methods, with the different areas presented in a clearly structured and easily accessible manner. 606 $aMice as laboratory animals 606 $aMice$xGenetics 606 $aTransgenic mice 606 $aPhenotype 615 0$aMice as laboratory animals. 615 0$aMice$xGenetics. 615 0$aTransgenic mice. 615 0$aPhenotype. 676 $a599.353135 701 $aHrabe? de Angelis$b Martin$01621348 701 $aChambon$b Pierre$01621349 701 $aBrown$b Stephen D. M$092951 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910830451103321 996 $aStandards of mouse model phenotyping$93954587 997 $aUNINA