LEADER 05025nam 2200649 450 001 9910830420003321 005 20230803205203.0 010 $a1-68367-342-5 010 $a1-68367-100-7 010 $a1-55581-884-6 035 $a(CKB)3710000000240637 035 $a(EBL)1789612 035 $a(SSID)ssj0001333391 035 $a(PQKBManifestationID)12492466 035 $a(PQKBTitleCode)TC0001333391 035 $a(PQKBWorkID)11396198 035 $a(PQKB)10656744 035 $a(MiAaPQ)EBC1789612 035 $a(OCoLC)1055349363$z(OCoLC)890983800$z(OCoLC)893708435$z(OCoLC)922699829 035 $a(EXLCZ)993710000000240637 100 $a20140925h20142014 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 00$aMolecular genetics of mycobacteria /$fedited by Graham F. Hatfull and William R. Jacobs, Jr 205 $a2nd edition. 210 1$aWashington, District of Columbia :$cASM Press,$d2014. 210 4$dİ2014 215 $a1 online resource (xviii, 805 pages) $cillustrations (some color) 300 $aDescription based upon print version of record. 311 $a1-55581-883-8 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aCover; Half Title; Title Page; Copyright; Contents; Contributors; Preface; I. Genomes, Genomics, and Genetic Exchange; Chapter 1: Gene Transfer in Mycobacterium tuberculosis: Shuttle Phasmids to Enlightenment; OVERVIEW OF KEY MUTATIONS THAT FACILITATED GENE TRANSFER IN M. TUBERCULOSIS; GENE TRANSMISSION ESTABLISHED PHENOTYPE CAUSALITY; ELUCIDATING THE MECHANISMS OFDRUG KILLING: THE STREPTOMYCIN EXAMPLE; TRANSFER OF GENES FROM PATHOGENIC MYCOBACTERIA INTO THE SURROGATE HOST E. COLI: GREAT PROMISE WITH LIMITATIONS 327 $aINTRODUCTION OF FOREIGN DNA INTO MYCOBACTERIA: SHUTTLE PHASMIDS AND THE DEVELOPMENT OF A PLASMID TRANSFORMATION SYSTEM FOR M. SMEGMATISSECOND GENERATION SHUTTLE PHASMIDS: REPORTER MYCOBACTERIOPHAGES, TRANSPOSON DELIVERY, AND SPECIALIZED TRANSDUCTION; HIGH-THROUGHPUT SPECIALIZED TRANSDUCTION; IMAGINING A WORLD WITHOUT TB; Chapter 2: Mycobacterial Pathogenomics and Evolution; INTRODUCTION; MYCOBACTERIAL GENOMICS; INSIGHTS INTO THE MACROEVOLUTION OF M. TUBERCULOSIS BASED ON COMPARISON WITH M. MARINUM AND MYCOBACTERIUM KANSASII; MICROEVOLUTIONARY GENOMICS OF THE TUBERCLE BACILLI 327 $aMYCOBACTERIAL SYSTEMS DISCOVERED BY GENOMICS THAT ARE INVOLVED IN PATHOGENICITYCONCLUSIONS AND PERSPECTIVES; Chapter 3: BCG Vaccines; HISTORY OF BCG SUBSTRAINS; BCG AS AN ATTENUATED MEMBER OF THE M. TUBERCULOSIS COMPLEX; MOLECULAR EVOLUTION AND THE DERIVATION OF BCG SUBSTRAINS; MOLECULAR EVOLUTION AND THE PROPAGATION OF BCG SUBSTRAINS; CONCLUSION AND PERSPECTIVES: ATTENUATION OF VIRULENCE AND PROTECTIVE EFFICACY; REFERENCES; Chapter 4: Distributive Conjugal Transfer: New Insights into Horizontal Gene Transfer and Genetic Exchange in Mycobacteria 327 $aCLASSICAL oriT-MEDIATED CONJUGATION: A BRIEF OVERVIEWMYCOBACTERIAL CONJUGATION; DISTRIBUTIVE CONJUGAL TRANSFER; DCT AS AN ENGINE FOR MYCOBACTERIAL EVOLUTION; DCT AS A GENETIC MAPPING TOOL; SUMMARY AND FUTURE PROSPECTSFOR DCT; Chapter 5: Molecular Genetics of Mycobacteriophages; GENERAL ASPECTS OF MYCOBACTERIOPHAGES; MYCOBACTERIOPHAGE GENOMICS; MYCOBACTERIOPHAGE-HOST INTERACTIONS; INTEGRATION SYSTEMS; MYCOBACTERIOPHAGE GENE EXPRESSIONAND ITS REGULATION; GENETIC MANIPULATION OF MYCOBACTERIOPHAGES; APPLICATIONS FOR MYCOBACTERIAL GENETICS; PERSPECTIVES: PAST, PRESENT, AND FUTURE 327 $aChapter 6: Genetics of Phage LysisTHE LYSIS PLAYERS; MYCOBACTERIOPHAGE-MEDIATED LYSIS; APPLICATIONS OF THE LYSIS PROPERTIES; CONCLUDING REMARKS; II. Gene Expression and Regulation; Chapter 7: Sigma Factors: Key Molecules in Mycobacterium tuberculosis Physiology and Virulence; THE ? FACTORS OF MYCOBACTERIUM TUBERCULOSIS ; Chapter 8: Transcription Factor Binding Site Mapping Using ChIP-Seq; ChIP-Seq METHOD AND PROTOCOL; DATA ANALYSIS; COMPREHENSIVE MAPPING OF TF BINDING SITES IN M. TUBERCULOSIS; VALIDATION OF THE ChIP-Seq SYSTEM; THE DIVERSITY OF TF BINDING IN PROKARYOTES 327 $aNONPROMOTER TF BINDING 330 $aA comprehensive collection of perspectives by experts in mycobacterial molecular biology, written by leading experts in the field. This book is an invaluable resource for anyone interested in the molecular genetics and molecular biology of mycobacteria. 606 $aMycobacteria 606 $aBacterial genetics 606 $aTuberculosis$xGenetic aspects 615 0$aMycobacteria. 615 0$aBacterial genetics. 615 0$aTuberculosis$xGenetic aspects. 676 $a579.374135 702 $aHatfull$b Graham F. 702 $aJacobs$b William R.$cJr, 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910830420003321 996 $aMolecular genetics of mycobacteria$94013829 997 $aUNINA