LEADER 05161nam 22006014a 450 001 9910830408703321 005 20230617040626.0 010 $a1-280-85416-2 010 $a9786610854165 010 $a3-527-60766-8 010 $a1-61583-208-4 010 $a3-527-60708-0 024 3 $z9783527312320 035 $a(CKB)1000000000375908 035 $a(EBL)481785 035 $a(OCoLC)70113405 035 $a(SSID)ssj0000118310 035 $a(PQKBManifestationID)11133249 035 $a(PQKBTitleCode)TC0000118310 035 $a(PQKBWorkID)10053081 035 $a(PQKB)11166711 035 $a(MiAaPQ)EBC481785 035 $a(EXLCZ)991000000000375908 100 $a20060525d2005 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aCarrier-bound immobilized enzymes$b[electronic resource] $eprinciples, applications and design /$fLinqiu Cao 210 $aWeinheim $cWiley-VCH$dc2005 215 $a1 online resource (581 p.) 300 $aDescription based upon print version of record. 311 $a3-527-31232-3 320 $aIncludes bibliographical references and index. 327 $aCarrier-bound Immobilized Enzymes; Foreword; Contents; 1 Introduction: Immobilized Enzymes: Past, Present and Prospects; 1.1 Introduction; 1.2 The Past; 1.2.1 The Early Days (1916-1940s); 1.2.2 The Underdeveloped Phase (1950s); 1.2.3 The Developing Phase (1960s); 1.2.4 The Developed Phase (1970s); 1.2.5 The Post-developed Phase (1980s); 1.2.6 Rational Design of Immobilized Enzymes (1990s-date); 1.3 Immobilized Enzymes: Implications from the Past; 1.3.1 Methods of Immobilization; 1.3.2 Diversity versus Versatility; 1.3.3 Complimentary versus Alternative 327 $a1.3.4 Modification versus Immobilization1.3.4.1 Enhanced Stability; 1.3.4.2 Enhanced Activity; 1.3.4.3 Improved Selectivity; 1.4 Prospective and Future Development; 1.4.1 The Room for Further Development; 1.4.2 An Integration Approach; 1.5 References; 2 Adsorption-based Immobilization; 2.1 Introduction; 2.2 Classification of Adsorption; 2.3 Principles Involved in Absorptive Enzyme Immobilization; 2.3.1 Monolayer Principle; 2.3.2 Stabilization Principle; 2.3.3 Enzyme Distribution; 2.4 Requirement of the Carriers; 2.4.1 Physical Requirements; 2.4.1.1 Pore-size and Available Surface 327 $a2.4.1.2 Internal Structure2.4.1.3 Density of Binding Functionality; 2.4.1.4 Particle Size; 2.4.2 Chemical Nature of the Carriers; 2.4.2.1 Nature of Binding Functionality; 2.4.2.2 The Role of the Spacer; 2.4.2.3 The Nature of the Backbone; 2.5 Factors Which Dictate Enzyme Catalytic Performance; 2.5.1 Activity; 2.5.1.1 Diffusion-controlled Activity; 2.5.1.2 Conformation-controlled Activity; 2.5.1.3 Substrate-controlled Activity; 2.5.1.4 Loading-controlled Activity; 2.5.1.5 Medium-dependent Activity; 2.5.1.6 Microenvironment-dependent Activity; 2.5.1.7 Carrier Nature-dependent Activity 327 $a2.5.1.8 Enzyme Nature-dependent Activity2.5.1.9 Additive-dependent Activity; 2.5.1.10 Hydrophilicity-dependent Activity; 2.5.1.11 Orientation-determined Activity; 2.5.1.12 Binding Nature-controlled Enzyme Activity; 2.5.1.13 Binding Density-controlled Enzyme Activity; 2.5.1.14 Reactor-dependent Activity; 2.5.1.15 Pore-size-dependent Activity; 2.5.1.16 Water-activity-dependent Activity; 2.5.2 Stability; 2.5.2.1 Conformation-controlled Stability; 2.5.2.2 Confinement-controlled Stability; 2.5.2.3 Enzyme Loading-dependent Stability; 2.5.2.4 Diffusion-controlled Stability 327 $a2.5.2.5 Cross-linking-dependent Stability2.5.2.6 Carrier Nature-controlled Stability; 2.5.2.7 Aquaphilicity-controlled Stability; 2.5.2.8 Medium-controlled Stability; 2.5.2.9 Temperature-dependent Stability; 2.5.2.10 Microenvironment-controlled Stability; 2.5.2.11 Binding Nature-controlled Enzyme Stability; 2.5.2.12 Binding Density-controlled Enzyme Stability; 2.5.2.13 Additive-dependent Stability; 2.5.2.14 Enzyme Orientation-dependent Stability; 2.5.2.15 Enzyme-dependent Stability; 2.5.3 Selectivity; 2.5.3.1 Conformation-controlled Selectivity; 2.5.3.2 Diffusion-controlled Selectivity 327 $a2.5.3.3 Binding Functionality-controlled Selectivity 330 $aThe first systematic overview of this key technique since the early 1990s, this authoritative reference is the only handbook available to include all recent developments. The author draws on his wide-ranging experience in both academia and industry to systematically cover all types of enzyme immobilization methods, such as adsorption-based and covalent immobilization, as well as enzyme entrapment and encapsulation. Throughout, a careful review of materials and techniques for the generation of functional immobilized enzymes benefits both developers and users of carrier-bound enzymes.A must 606 $aImmobilized enzymes 615 0$aImmobilized enzymes. 676 $a572.7 700 $aCao$b Linqiu$01595385 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910830408703321 996 $aCarrier-bound immobilized enzymes$93916309 997 $aUNINA