LEADER 00733nam0-22002651i-450 001 990003749430403321 005 20220721161442.0 035 $a000374943 035 $aFED01000374943 035 $a(Aleph)000374943FED01 035 $a000374943 100 $a20001010d1975----km-y0itay50------ba 101 0 $aita 105 $ay-------001yy 200 1 $aDirectory (fifth international) of Anthropologist 210 $aChicago$cUniv. of Chicago Press$d1975 . 215 $a496 p.$d28 cm 801 0$aIT$bUNINA$gRICA$2UNIMARC 901 $aBK 912 $a990003749430403321 952 $aA / DIR 1$b3160$fBFS 959 $aBFS 996 $aDIRECTORY (fifth international) of Anthropologist$9508373 997 $aUNINA DB $aING01 LEADER 03744oam 2200349z- 450 001 9910830210003321 005 20210108010642.0 010 $a1-118-66324-1 010 $a1-118-66326-8 010 $a1-118-66323-3 035 $a(CKB)4330000000006727 035 $a(MiAaPQ)EBC5672603 035 $a(OCoLC)1035317057 035 $a(EXLCZ)994330000000006727 100 $a20200217c2019uuuu -u- - 101 0 $aeng 135 $aur||||||||||| 200 00$aBiological field emission scanning electron microscopy /$fedited by Roland A. Fleck, Bruno M. Humbel 210 $cJohn Wiley & Sons, Inc 311 $a1-118-65406-4 330 $a"The go-to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope - giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy--specifically, those with a need to understand the application, limitations, and strengths of FEGSEM"--Provided by publisher. 606 $aMicroscopy, Electron, Scanning$xmethods 606 $aMicroscopy, Electron, Scanning$xinstrumentation 615 12$aMicroscopy, Electron, Scanning$xmethods 615 12$aMicroscopy, Electron, Scanning$xinstrumentation 676 $a502.8/25 702 $aFleck$b Roland A.$f1970- 702 $aHumbel$b Bruno M. 906 $aBOOK 912 $a9910830210003321 996 $aBiological field emission scanning electron microscopy$94032764 997 $aUNINA