LEADER 00887nam0-2200313---450- 001 990010031090403321 005 20160122090616.0 010 $a978-88-348-3799-3 035 $a001003109 035 $aFED01001003109 035 $a(Aleph)001003109FED01 035 $a001003109 100 $a20160122d2012----km-y0itay50------ba 101 0 $aita 102 $aIT 105 $ay-------001yy 200 1 $a<>comitati nella corporate governance in Europa$fFrancesca Gennari 210 $aTorino$cGiappichelli$d2012 215 $aX, 108 p.$d24 cm 225 1 $aImpresa Economia Comunicazione$v28 676 $a658 700 1$aGennari,$bFrancesca$0437487 801 0$aIT$bUNINA$gRICA$2UNIMARC 901 $aBK 912 $a990010031090403321 952 $aGEN658A$b108$fDECBC 959 $aDECBC 996 $aComitati nella corporate governance in Europa$91496033 997 $aUNINA LEADER 05109nam 22005894a 450 001 9910830071403321 005 20230617032030.0 010 $a1-280-27051-9 010 $a9786610270514 010 $a0-470-85651-3 010 $a0-470-85652-1 035 $a(CKB)111056485598012 035 $a(EBL)148940 035 $a(OCoLC)807994952 035 $a(SSID)ssj0000218586 035 $a(PQKBManifestationID)11208259 035 $a(PQKBTitleCode)TC0000218586 035 $a(PQKBWorkID)10220433 035 $a(PQKB)11496628 035 $a(MiAaPQ)EBC148940 035 $a(EXLCZ)99111056485598012 100 $a20020813d2003 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aPatch clamping$b[electronic resource] $ean introductory guide to patch clamp electrophysiology /$fAreles Molleman 210 $aNew York $cJ. Wiley$dc2003 215 $a1 online resource (187 p.) 300 $aDescription based upon print version of record. 311 $a0-471-48685-X 320 $aIncludes bibliographical references and index. 327 $aPatch Clamping; Contents; Preface; 1 Introduction; 1.1 Patch Clamping and its Context; 2 Basic Theoretical Principles; 2.1 Introduction to Membrane Biology; 2.1.1 The plasma membrane and its ionic environment; 2.1.2 Electrochemical gradients and the Nernst equation; 2.1.3 Maintenance of ion gradients and the membrane potential; 2.1.4 Ion channels; 2.2 Electrical Properties of the Cell Membrane; 2.2.1 Driving force and membrane resistance; 2.2.2 Membrane capacitance; 2.2.3 Consequences of membrane capacitance; 2.2.4 An electronic model of the plasma membrane 327 $a2.3 Recording Modes and their Equivalent Circuits2.3.1 The basics of equivalent circuits; 2.3.2 Intracellular recording; 2.3.3 Voltage clamp and current clamp; 2.3.4 Introduction to patch clamp configurations; 2.3.5 The equivalent circuit for the cell-attached patch configuration; 2.3.6 The equivalent circuit for the whole-cell configuration; 2.3.7 The equivalent circuit for the excised patch configurations; 3 Requirements; 3.1 The Platform; 3.1.1 Stability: vibrations and drift; 3.1.2 Where in the building should the set-up be placed?; 3.1.3 Anti-vibration tables; 3.2 Mechanics and Optics 327 $a3.2.1 The microscope3.2.2 Micromanipulators; 3.2.3 Pipette pressure; 3.2.4 Baths and superfusion systems; 3.3 Electrodes and Micropipettes; 3.3.1 Solid-liquid junction potentials and polarisation; 3.3.2 The bath electrode; 3.3.3 Micropipettes; 3.3.4 Liquid junction potentials; 3.4 Electronics; 3.4.1 External noise and Faraday cages; 3.4.2 Patch clamp amplifiers; 3.4.3 Noise prevention and signal conditioning; 3.4.4 Data acquisition and digitisation; 3.4.5 Computers and software; 4 The Practice of Patch Clamping; 4.1 Preparing the Experiment and Making a Seal; 4.1.1 Setting up 327 $a4.1.2 Bringing the pipette near the preparation4.1.3 Making the seal; 4.2 Whole-cell Modes; 4.2.1 Conventional whole-cell recording; 4.2.2 Perforated patch recording; 4.3 Single-channel Modes; 4.3.1 General notes; 4.3.2 Cell-attached patch; 4.3.3 Excised patches; 5 Whole-cell Protocols and Data Analysis; 5.1 Standard Cellular Parameters; 5.2 Voltage-activated Currents; 5.2.1 Introduction to pulse protocols; 5.2.2 Signal conditioning and positive/negative subtraction; 5.2.3 Space clamp artefacts; 5.2.4 Isolation of a homogeneous population of channels 327 $a5.2.5 Current-voltage relationships and reversal potential5.2.6 Determination of relative permeabilities; 5.2.7 Activation and inactivation studies; 5.3 Non-voltage-activated Currents; 5.3.1 Introduction to continuous recording; 5.3.2 Determination of reversal potential using voltage ramps; 6 Single-channel Protocols and Data Analysis; 6.1 General Single-channel Practice and Analysis; 6.1.1 Practical notes; 6.1.2 Amplitude analysis; 6.1.3 Event detection; 6.1.4 Dwell time analysis; 6.2 Continuous Recording of Single Channels; 6.2.1 Data acquisition; 6.2.2 Spontaneous activity 327 $a6.2.3 Receptor-induced activity 330 $aPatch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells. Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results. < 606 $aPatch-clamp techniques (Electrophysiology) 615 0$aPatch-clamp techniques (Electrophysiology) 676 $a571.64 676 $a572/.437 700 $aMolleman$b Areles$0471690 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910830071403321 996 $aPatch clamping$9229524 997 $aUNINA