LEADER 05393nam 2200661 450 001 9910830044503321 005 20230721033333.0 010 $a1-281-94689-3 010 $a9786611946890 010 $a3-527-62248-9 010 $a3-527-62249-7 035 $a(CKB)1000000000554041 035 $a(EBL)481324 035 $a(OCoLC)277155574 035 $a(SSID)ssj0000106997 035 $a(PQKBManifestationID)11122402 035 $a(PQKBTitleCode)TC0000106997 035 $a(PQKBWorkID)10009011 035 $a(PQKB)11591459 035 $a(MiAaPQ)EBC481324 035 $a(EXLCZ)991000000000554041 100 $a20160819h20082008 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aAsymmetric organic synthesis with enzymes /$fedited by Vicente Gotor, Ignacio Alfonso, and Eduardo Garci?a-Urdiales 210 1$aWeinheim, Germany :$cWILEY-VCH Verlag GmbH & Co. KGaA,$d2008. 210 4$dİ2008 215 $a1 online resource (341 p.) 300 $aDescription based upon print version of record. 311 $a3-527-31825-9 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aAsymmetric Organic Synthesis with Enzymes; Contents; Preface; List of Contributors; I Methodology; 1 Medium Engineering; 1.1 Introduction; 1.2 Modulation of Enzyme Enantioselectivity by Medium Engineering; 1.2.1 Selectivity Enhancement by Addition of Water-Miscible Organic Cosolvents; 1.2.2 Selectivity Enhancement in Organic Media with Low Water Activity; 1.2.2.1 Organic Solvent Systems; 1.2.2.2 Enzyme Properties in Organic Solvents; 1.2.2.3 Medium Engineering; 1.2.3 Rationales; 1.2.4 Modulation of Enzyme Selectivity: New Trends of Research; 1.2.4.1 Ionic Liquids; 1.2.4.2 Additives 327 $a1.3 Conclusions and OutlooksReferences; 2 Directed Evolution as a Means to Engineer Enantioselective Enzymes; 2.1 Introduction; 2.2 Molecular Biological Methods for Mutagenesis; 2.3 High-throughput Screening Methods for Enantioselectivity; 2.4 Examples of Enhancing the Enantioselectivity of Enzymes by Directed Evolution; 2.4.1 Lipase from Pseudomonas aeruginosa (PAL); 2.4.2 Other Lipases; 2.4.3 Esterases; 2.4.4 Hydantoinases; 2.4.5 Nitrilases; 2.4.6 Epoxide Hydrolases; 2.4.7 Phosphotriesterases; 2.4.8 Aminotransferases; 2.4.9 Aldolases 327 $a2.4.10 Cyclohexanone and Cyclopentanone Monooxygenases as Baeyer-Villigerases and Sulfoxidation Catalysts2.4.11 Monoamine Oxidases; 2.4.12 Cytochrome P450 Enzymes; 2.4.13 Other Enzymes; 2.5 Conclusions and Perspectives; References; 3 The Search for New Enzymes; 3.1 Introduction; 3.2 Mechanism-based Enzyme Design; 3.2.1 Catalytic Antibodies; 3.2.2 Rational Design of New Catalysts on Enzyme and Protein Basis; 3.2.3 Synthetic Enzyme Models; 3.3 Metagenomics; 3.3.1 Construction of Metagenome-derived DNA Libraries; 3.3.1.1 Selection of the Environment; 3.3.1.2 Cloning Strategies 327 $a3.3.1.3 Screening and Detection Technologies3.3.1.4 Major Problems that Need to be Addressed; 3.3.2 The Genomes of Not Yet Cultured Microbes as Resources for Novel Genes; 3.3.2.1 Polysaccharide Degrading/Modifying Enzymes; 3.3.2.2 Lipolytic Biocatalysts; 3.3.2.3 Vitamin Biosynthesis; 3.3.2.4 Nitrilases, Nitrile Hydratases, and Amidases; 3.3.2.5 Oxidoreductases/Dehydrogenases; 3.3.2.6 Proteases; 3.3.2.7 Glycerol Hydratases; 3.3.2.8 Antibiotics and Pharmaceuticals; 3.4 Conclusion; References; II Synthetic Applications; 4 Dynamic Kinetic Resolutions; 4.1 Introduction 327 $a4.1.1 Synthesis of Enantiomerically Pure Compounds4.1.2 Kinetic Resolution (KR) and Dynamic Kinetic Resolution (DKR); 4.1.3 Enzymes in Organic Chemistry; 4.2 Metal-Catalyzed Racemization; 4.2.1 DKR of Allylic Acetates and Allylic Alcohols; 4.2.2 DKR of sec-alcohols; 4.2.3 DKR of Amines; 4.3 Base-Catalyzed Racemization; 4.3.1 DKR of Thioesters; 4.3.2 DKR of Activated Esters; 4.3.3 DKR of Oxazolones; 4.3.4 DKR of Hydantoins; 4.3.5 DKR of Acyloins; 4.4 Acid-Catalyzed Racemization; 4.5 Racemization through Continuous Reversible Formation-Cleavage of the Substrate; 4.5.1 DKR of Cyanohydrins 327 $a4.5.2 DKR of Hemithioathetals 330 $aPerfect for biochemists, synthetic and organic chemists, this book covers all important reactions, including C-C coupling reactions, oxidation reactions and many more. Divided into two parts, the first section on methodology presents new innovative methods for enzymatic catalysis optimization, including such new trends as medium engineering, directed evolution and computer-aided prediction of enantioselectivity. The second and main section deals with applications to synthesis, showing important reaction types and their applications. Only those reactions with very high selectivity are prese 606 $aEnzymes$xSynthesis 606 $aAsymmetric synthesis 606 $aOrganic compounds$xSynthesis 615 0$aEnzymes$xSynthesis. 615 0$aAsymmetric synthesis. 615 0$aOrganic compounds$xSynthesis. 676 $a572.745 702 $aGotor$b Vicente 702 $aAlfonso$b Ignacio 702 $aGarci?a-Urdiales$b Eduardo 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910830044503321 996 $aAsymmetric organic synthesis with enzymes$94049162 997 $aUNINA