LEADER 05521nam 2200673 450 001 9910829991203321 005 20230721030218.0 010 $a1-282-78421-8 010 $a9786612784217 010 $a3-527-62122-9 010 $a3-527-62123-7 035 $a(CKB)1000000000377235 035 $a(EBL)482222 035 $a(OCoLC)609855555 035 $a(SSID)ssj0000353901 035 $a(PQKBManifestationID)11246031 035 $a(PQKBTitleCode)TC0000353901 035 $a(PQKBWorkID)10302740 035 $a(PQKB)10873724 035 $a(MiAaPQ)EBC482222 035 $a(EXLCZ)991000000000377235 100 $a20160820h20082008 uy 0 101 0 $ager 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aBiophysical analysis of membrane proteins $einvestigating structure and function /$fedited by Eva Pebay-Peyroula 210 1$aWeinheim, [Germany] :$cWiley-VCH Verlag GmbH & Co. KGaA,$d2008. 210 4$dİ2008 215 $a1 online resource (369 p.) 300 $aDescription based upon print version of record. 311 $a3-527-31677-9 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aBiophysical Analysis of Membrane Proteins; Contents; Preface; The Editor; List of Contributors; Part I Introduction; 1 High-Resolution Structures of Membrane Proteins: From X-Ray Crystallography to an Integrated Approach of Membranes; 1.1 Membranes: A Soft Medium?; 1.2 Current Knowledge on Membrane Protein Structures; 1.2.1 An Overview of the Protein Data Bank; 1.2.2 Protein Sources for Structural Studies; 1.2.3 The Diversity of Membrane Protein Topologies; 1.2.4 Genome Analyses; 1.3 X-Ray Crystallography; 1.3.1 Crystallization of Membrane Proteins; 1.3.2 General Aspects of Crystallography 327 $a1.3.3 Determining the Phases Associated with Diffracted Waves1.3.4 Structure Determination of Membrane Proteins; 1.3.4.1 Crystal Quality; 1.3.4.2 Phase Determination; 1.3.4.3 Crystal Freezing; 1.4 Recent Examples; 1.4.1 Bacterial Rhodopsins; 1.4.2 ADP/ATP Carrier; 1.4.3 Oligomerization of Membrane Proteins in their Natural Environment; 1.5 Future Developments in X-Ray Crystallography of Membrane Proteins; 1.6 Conclusions; Part II Structural Approaches; 2 Membrane Protein Structure Determination by Electron Cryo-Microscopy; 2.1 Introduction; 2.1.1 The Electron Microscope 327 $a2.2 Single-Particle Electron Microscopy2.2.1 Sample Preparation and Requirements; 2.2.1.1 Negative Staining of Specimens; 2.2.1.2 Cryo-EM of Unstained Specimens; 2.2.1.3 Choice of detergent; 2.2.2 Image Analysis; 2.2.2.1 Classification of Images; 2.2.2.2 Model Building and Refinement; 2.2.2.3 Assessing Resolution; 2.2.3 Future Perspectives; 2.3 Structure Determination from 2-Dimensional Crystals; 2.3.1 Two-Dimensional Crystallization of Membrane Proteins; 2.3.2 Image Acquisition and Structure Determination; 2.3.3 Future Perspectives; 2.4 Helical Analysis of Tubes; 2.5 Conclusions 327 $a3 Introduction to Solid-State NMR and its Application to Membrane Protein-Ligand Binding Studies3.1 Introduction; 3.1.1 Membrane Proteins: A Challenge; 3.1.2 Why Solid-State NMR?; 3.2 Solid-State NMR; 3.2.1 Sample Preparation: What is an Ideal Sample?; 3.2.1.1 Availability; 3.2.1.2 Stability; 3.2.1.3 Secondary Structure; 3.2.1.4 Sample Form: Local Order; 3.2.2 NMR Active Isotopes and Labeling; 3.2.3 Assignment and Structure Determination; 3.2.4 NMR Techniques: Solution- versus Solid-State NMR; 3.2.4.1 Isotropic Liquids; 3.2.4.2 Anisotropic Liquids; 3.2.4.3 Solids 327 $a3.3 Examples: Receptor-Ligand Studies by Solid-State NMR3.3.1 Transport Proteins; 3.3.1.1 LacS; 3.3.2 G-Protein-Coupled Receptors and Related Proteins; 3.3.2.1 Bacteriorhodopsin, Rhodopsin, and Sensory Rhodopsin (NpSRII); 3.3.2.2 Human H(1) Receptor; 3.3.2.3 Neurotensin Receptor; 3.3.3 Ion Channels; 3.3.3.1 Nicotinic Acetylcholine Receptor; 3.3.3.2 K(+) Ion Channel, KcsA; 3.3.4 P-type ATPases; 3.3.5 Membrane Protein Soluble Alternatives; Part III Molecular Interaction and Large Assemblies; 4 Analytical Ultracentrifugation: Membrane Protein Assemblies in the Presence of Detergent 327 $a4.1 Introduction 330 $aMeeting the need for a book on developing and using new methods to investigate membrane proteins, this is the first of its kind to present the full range of novel techniques in one resource. Top researchers from around the world focus on the physical principles exploited in the different techniques, and provide examples of how these can bring about important new insights.Following an introduction, further sections discuss structural approaches, molecular interaction and large assemblies, dynamics and spectroscopies, finishing off with an exploration of structure-function relationships in w 606 $aMembrane proteins$xStructure-activity relationships 606 $aMembrane proteins$xAnalysis 606 $aCell membranes$xPhysiology 606 $aPhysical biochemistry 615 0$aMembrane proteins$xStructure-activity relationships. 615 0$aMembrane proteins$xAnalysis. 615 0$aCell membranes$xPhysiology. 615 0$aPhysical biochemistry. 676 $a572.6 676 $a572/.69 702 $aPebay-Peyroula$b Eva 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910829991203321 996 $aBiophysical analysis of membrane proteins$94078848 997 $aUNINA