LEADER 05392nam 2200649 450 001 9910829855603321 005 20230421044549.0 010 $a1-281-84290-7 010 $a9786611842901 010 $a3-527-61581-4 010 $a3-527-61580-6 035 $a(CKB)1000000000377159 035 $a(EBL)481280 035 $a(OCoLC)289077943 035 $a(SSID)ssj0000204844 035 $a(PQKBManifestationID)11184291 035 $a(PQKBTitleCode)TC0000204844 035 $a(PQKBWorkID)10191489 035 $a(PQKB)11589339 035 $a(MiAaPQ)EBC481280 035 $a(EXLCZ)991000000000377159 100 $a20160819h19941994 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aMolecular ecology of rhizosphere microorganisms $ebiotechnology and the release of GMOs /$fedited by F. O'Gara, D. N. Dowling, B. Boesten 210 1$aWeinheim, Germany :$cVCH,$d1994. 210 4$dİ1994 215 $a1 online resource (193 p.) 300 $aDescription based upon print version of record. 311 $a3-527-30052-X 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aMolecular Ecology of Rhizosphere Microorganisms; Preface; List of Contributors; Content; 1 Current Challenges in Introducing Beneficial Microorganisms into the Rhizosphere; 1.1 Introduction and Definitions; 1.2 Relationship of Root Colonization to Biocontrol and Growth Promotion; 1.3 The Process of Colonization; 1.4 Effect of Biotic and Abiotic Factors; 1.5 Bacterial Traits Contributing to Rhizosphere Competence; 1.6 Population Dynamics of PGPR in the Field; 1.7 Release of Genetically Engineered Rhizobacteria; 1.8 Mechanisms of Biological Control by PGPR; 1.9 Inconsistant Performance of PGPR 327 $a1.10 Improving Root Colonizing and Biological Control1.11 Conclusion; 1.12 References; 2 Studies on Indigenous Endophytic Bacteria of Sweet Corn and Cotton; 2.1 Introduction; 2.2 Materials and Methods; 2.2.1 Media; 2.2.2 Field Experiments; 2.2.3 Sample Preparation and Surface Sterilization; 2.2.4 Growth Conditions, Bacterial Counts and Data Analysis; 2.2.5 Isolation and Preservation of Endophytes; 2.2.6 Strain Identification; 2.3 Results; 2.3.1 Population Dynamics; 2.3.2 Bacterial Identification; 2.4 Discussion; 2.5 References 327 $a3 Detection of Introduced Bacteria in the Rhizosphere Using Marker Genes and DNA Probes3.1 Introduction; 3.2 Methods; 3.2.1 Spontaneous Antibiotic Resistance; 3.2.2 Marker Genes; 3.2.2.1 New metabolic capability; 3.2.2.2 Heavy metal resistance; 3.2.2.3 Bioluminescence; 3.2.2.4 Herbicide resistance; 3.2.2.5 Transposons carrying antibiotic resistance; 3.2.3 DNA Probes; 3.2.4 Detection Limits, Amplification and Enrichment; 3.2.4.1 Increased Sensitivity by PCR Amplification; 3.2.4.2 Enrichment; 3.3 Case Study : ""kicking LacZY-labelled Pseudomonus cormgutu in the Field; 3.3.1 Pre-release Testing 327 $a3.3.2 Field Release3.4 The Ecological Fitness of Genetically-Engineered Bacteria; 3.4.1 Metabolic Load; 3.4.2 Reduced Fitness; 3.5 Conclusions; 3.6 References; 4 Impact of GEMMOs on Rhizosphere Population Dynamics; 4.1 Introduction; 4.2 A Most Probable Number (MPN) Recovery Technique; 4.3 The Need for an Eco-Physiological Index (EPI); 4.4 Conclusions; 4.5 References; 5 Developing Concepts in Biological Control: A Molecular Ecology Approach; 5.1 Introduction; 5.2 Siderophore-Mediated Competitive Exclusion of Phytopathogens; 5.3 Exploiting Antifungal Metabolites to Enhance Biological Control 327 $a5.4 Stability of Introduced Genes and Biological Containment Systems for GMO's5.5 Conclusion; 5.6 References; 6 Biocontrol of Root Diseases by Pseudomonas fluorescens CHAO: Current Concepts and Experimental Approaches; 6.1 Introduction; 6.2 Mechanistic Studies on Biocontrol Traits of Pseudomonus Fluorescens CHAO; 6.2.1 Chemical Identification of Extracellular Metabolites; 6.2.2 Genetic Manipulation of Strain CHAO; 6.2.3 Gnotobiotic System; 6.2.4 Mutations Affecting Biocontrol Efficacy, Regulation of Secondary Metabolism, and some Caveats; 6.2.5 Induced Systemic Resistance in Plants 327 $a6.2.6 Genetic Instability of Strain CHAO: Effects on Secondary Metabolism and Biological Control 330 $aThis book helps evaluate the state of the art of rhizosphere microbial ecology and biotechnology. Experts in the field review methods and strategies applied to the detection, identification and monitoring of microorganisms in the rhizosphere. Major topics treated include:- construction of genetically marked rhizosphere bacteria - detection of marked wildtype and genetically modified organisms (GMOs)- identification of wildtype and GMOs by DNA probes and PCR amplification- rapid typing of non-modified and GMOs by PCR-based techniques - assessment of the role of gene transfer 606 $aRhizosphere$vCongresses 606 $aMicrobial ecology$vCongresses 615 0$aRhizosphere 615 0$aMicrobial ecology 676 $a576 676 $a576.15 702 $aO'Gara$b F. 702 $aDowling$b D. 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