LEADER 00968nam1 2200337 450 001 990000356930203316 035 $a0035693 035 $aUSA010035693 035 $a(ALEPH)000035693USA01 035 $a0035693 100 $a20010313d--------km-y0itay0103----ba 101 $afra 102 $aFR 105 $a||||||||001yy 200 1 $aMéthodos et modèles de la recherche opérationnelle$eLes methématiques de l'entreprise$fArnold Kaufmann 210 $aParis$cDunod$ds.d. 215 $avol$d23 cm 410 $12001 461 1$1001-------$12001 676 $a001.424 700 1$aKAUFMANN$bArnold$014433 801 0$aIT$bsalbc$gISBD 912 $a990000356930203316 951 $a001.424$c001 959 $aBK 969 $aSCI 979 $aPATTY$b90$c20010313$lUSA01$h1111 979 $c20020403$lUSA01$h1643 979 $aPATRY$b90$c20040406$lUSA01$h1625 996 $aMéthodos et modèles de la recherche opérationnelle$9876521 997 $aUNISA LEADER 01194nam 2200325Ia 450 001 996384505203316 005 20221108063114.0 035 $a(CKB)4940000000075552 035 $a(EEBO)2248542442 035 $a(OCoLC)12198509 035 $a(EXLCZ)994940000000075552 100 $a19850625d1649 uy | 101 0 $aeng 135 $aurbn||||a|bb| 200 14$aThe spiritvall man's directory$b[electronic resource] $eguiding a Christian in the path that leads to true blessednesse in his III maine duties toward God : how to believe, to obey, to pray, unfolding the creed, X. command. 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Gjerde, Lee Hoang and David Hornby 210 $aWeinheim $cWiley-VCH ;$aChichester $cJohn Wiley [distributor]$dc2009 215 $a1 online resource (209 p.) 300 $aDescription based upon print version of record. 311 $a3-527-32116-0 320 $aIncludes bibliographical references and index. 327 $aRNA Purification and Analysis: Sample Preparation, Extraction, Chromatography; Contents; Preface; Acknowledgments; 1: RNA Extraction, Separation, and Analysis; 1.1 The Need to Be Able to Extract, Manipulate, and Analyze RNA; 1.2 Using Chemical Tools to Solve the Problem of Analysis of Biological Processes; 1.3 The Principle of Chromatography and Solid-Phase Extraction; 1.3.1 Principle of Chromatography; 1.3.2 Mobile Phase Gradient Controls Elution; 1.3.3 Different Types of Column and Eluent Chemistries; 1.3.4 The Principle of Solid-Phase Extraction; 1.4 RNA Chromatography 327 $a1.5 Enzymatic Treatment of RNA and Analysis1.5.1 Polyacrylamide Gel Electrophoresis; 1.5.2 RNA Structure Probing with Ribonuclease Enzymes; 1.6 Content and Organization of This Book; References; 2: Biological and Chemical RNA; 2.1 Why Classify RNA with Biology and Chemistry?; 2.1.1 Chemical Classification of RNA; 2.1.2 Biological Classification of RNA; 2.2 Prokaryotic Cellular RNA; 2.3 Prokaryote Sample Type; 2.3.1 Escherichia coli; 2.3.2 Other Bacteria; 2.4 Eukaryotic Cellular RNA; 2.5 Eukaryote Sample Type; 2.5.1 Yeast; 2.5.2 Other Fungi; 2.5.3 Simple Multicellular Organism 327 $a2.5.4 Soft Animal2.5.5 Hard Animal; 2.5.6 Plant; 2.5.7 Cell Culture; 2.6 Other Samples; 2.6.1 Virus; 2.6.2 Soil and Rock; 2.7 Synthetic RNA; 2.7.1 Aptamers; 2.7.2 SELEX; 2.7.3 Short Hairpin RNAs; References; 3: RNA Separation: Substrates, Functional Groups, Mechanisms, and Control; 3.1 Solid-Phase Interaction; 3.1.1 Adsorption of Sample Compounds and Sample Matrix Compounds; 3.1.2 Roles of Solid-Phase Substrate and Functional Group; 3.1.3 Correlation of Interaction Type, Functional Group, and Substrate; 3.1.4 RNA Structure and Solid Surface Interaction 327 $a3.2 The Solid-Phase Substrate and Attachment of Functional Groups3.2.1 Polymeric Resin Substrates; 3.2.2 Porous and Nonporous Polymeric Resins; 3.2.3 Monolith Polymeric Columns; 3.2.4 Functionalization of the Polymer; 3.2.5 Silica-Glass-Based Substrates; 3.2.6 Functionalization of Silica; 3.2.7 Agarose and Cellulose Affinity Substrates; 3.2.8 Dextran and Polyacrylamide Gel Filtration Substrates; 3.3 Reverse-Phase Ion-Pairing Separation Mechanism; 3.4 Ion-Exchange Separation Mechanism; 3.5 Chaotropic Denaturing Interaction Mechanism; 3.6 Hybridization; 3.6.1 SELEX; 3.7 Gel Filtration 327 $aReferences4: RNA Extraction and Analysis; 4.1 Transcription; 4.1.1 RNA Catalysis; 4.1.2 RNA-Protein Complex Interactions; 4.1.3 Pre-mRNA Splicing; 4.2 Translation; 4.2.1 Post-Transcriptional Control of Eukaryotic Gene Expression; 4.3 Gene Regulation; 4.3.1 RNA Interference Pathway; 4.3.2 Micro RNAs and Their Role in Gene Regulation; 4.4 Use of siRNA to Investigate Gene Function; References; 5: RNA Chromatography; 5.1 Development of RNA Chromatography; 5.2 RNA Chromatography Instrumentation; 5.2.1 The Column Oven; 5.2.2 Ultraviolet (UV) and Fluorescence Detection; 5.2.3 Fragment Collection 327 $a5.3 RNA Chromatography Conditions 330 $aThis first book on the market covers the many new and important RNA species discovered over the past five years, explaining current methods for the enrichment, separation and purification of these novel RNAs.Building up from general principles of RNA biochemistry and biophysics, this book addresses the practical aspects relevant to the laboratory researcher throughout, while discussing the performance and potential problems of the methods discussed. 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