LEADER 05099nam 2200613Ia 450 001 9910829843303321 005 20230617041607.0 010 $a1-280-52020-5 010 $a9786610520206 010 $a3-527-60366-2 010 $a3-527-60505-3 035 $a(CKB)1000000000375898 035 $a(EBL)482106 035 $a(OCoLC)85820480 035 $a(SSID)ssj0000118717 035 $a(PQKBManifestationID)11145942 035 $a(PQKBTitleCode)TC0000118717 035 $a(PQKBWorkID)10053646 035 $a(PQKB)10560458 035 $a(MiAaPQ)EBC482106 035 $a(EXLCZ)991000000000375898 100 $a20041222d2005 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aCatalytic antibodies$b[electronic resource] /$fedited by Ehud Keinan 210 $aWeinheim $cWiley-VCH ;$a[Chichester $cJohn Wiley]$d2005 215 $a1 online resource (618 p.) 300 $aDescription based upon print version of record. 311 $a3-527-30688-9 320 $aIncludes bibliographical references and index. 327 $aCatalytic Antibodies; Foreword; Table of Contents; Preface; List of Contributors; 1 Immunological Evolution of Catalysis; 1.1 Introduction; 1.2 Parallels between Antibody and Enzyme Evolution; 1.3 Evolution of Catalytic Antibodies; 1.4 Ferrochelatase Antibody 7G12 - Evolution of the Strain Mechanism; 1.5 Esterase Antibody 48G7 - Effect of Distant Mutations on Catalysis; 1.6 Sulfur Oxidase Antibody 28B4 - Incremental Changes in Evolution; 1.7 Oxy-Cope Antibody AZ28 - Evolution of Conformational Diversity in Catalysis 327 $a1.8 Diels-Alderase Antibody 39A11 - Evolution of a Polyspecific Antibody combining Site1.9 Conclusions; References; 2 Critical Analysis of Antibody Catalysis; 2.1 Introduction; 2.2 Exploiting Antibodies as Catalysts; 2.3 Catalytic Efficiency; 2.4 Hapten Design; 2.5 Representative Catalytic Antibodies; 2.5.1 Proximity Effects; 2.5.1.1 Sigmatropic Rearrangements; 2.5.1.2 Cycloadditions; 2.5.2 Strain; 2.5.2.1 Ferrochelatase Mimics; 2.5.2.2 Other Systems; 2.5.3 Electrostatic Catalysis; 2.5.3.1 Acyl Transfer Reactions; 2.5.4 Functional Groups; 2.5.4.1 Aldolases; 2.6 Perspectives 327 $a2.6.1 General Lessons from Comparisons of Enzymes and Antibodies2.6.2 How efficient does catalysis need to be?; 2.6.3 Strategies for Optimizing Efficiency; 2.6.3.1 Better Haptens; 2.6.3.2 Screening; 2.6.3.3 Engineering; 2.6.3.4 Selection; 2.6.3.5 Other Scaffolds; 2.7 Conclusions; References; 3 Theoretical Studies of Antibody Catalysis; 3.1 Introduction; 3.2 Questions Subject to Theoretical Elucidation; 3.2.1 Predicting Antibody Structure from Sequence; 3.2.2 Predicting Binding Modes and Binding Energies; 3.2.3 Understanding Antibody Catalysis [14]; 3.2.4 General Considerations 327 $a3.3 Hydrolytic Antibodies3.3.1 Gas and Solution Phase Hydrolysis of Aryl Esters; 3.3.2 Hapten Fidelity; 3.3.3 Theoretical Exploration of Antibody Catalysis; 3.3.3.1 16G3; 3.3.3.2 6D9; 3.3.3.3 43C9; 3.3.3.4 CNJ206; 3.3.3.5 48G7; 3.3.3.6 17E8 and 29G11; 3.4 Cationic Cyclizations; 3.4.1 Antibody Catalysis of Solvolysis; 3.4.2 Antibody-Catalyzed Hydroxyepoxide Cyclization; 3.5 Antibody-Catalyzed Diels-Alder and retro-Diels-Alder Reactions; 3.5.1 The Most Efficient endo-Diels-Alderase 1E9; 3.5.2 endo-Diels-Alderase 39A11 and its Germline Precursor; 3.5.3 exo-Diels-Alderase 13G5 327 $a3.5.4 retro-Diels-Alderase 10F113.6 Other Antibody-Catalyzed Pericyclic Reactions; 3.6.1 Oxy-Cope Rearrangement Catalyzed by Antibody AZ-28; 3.6.2 1,3-Dipolar Cycloaddition Catalyzed by Antibody 29G12; 3.6.3 Chorismate-Prephenate Claisen Rearrangement Catalyzed by Antibody 1F7; 3.7 Antibody-Catalyzed Carboxybenzisoxazole Decarboxylation; 3.8 Summary; References; 4 The Enterprise of Catalytic Antibodies: A Historical Perspective; 4.1 Introduction; 4.2 Methods; 4.3 Results; 4.3.1 The Conceptual Origins of Catalytic Antibodies; 4.3.2 Tapping the Immune System for Catalysts; 4.4 Conclusions 327 $aReferences 330 $aExploiting the inherent combinatorial mechanism in the biosynthesis of antibodies, an almost limitless variety of biocatalysts may be generated. Catalytic antibodies are capable of performing almost any type of reaction with high selectivity and stereospecificity.Here, the pioneers in the use of catalytic antibodies review the entire scope of this interdisciplinary field, covering such topics as:* theoretical aspects of structure, mechanism and kinetics* practical considerations, from immunization techniques to screening methods* in vitro evolution and other modern approaches