LEADER 05196nam 2200649 450 001 9910828718203321 005 20230925233749.0 010 $a1-908230-95-9 035 $a(CKB)3710000000077354 035 $a(EBL)1909044 035 $a(SSID)ssj0001164708 035 $a(PQKBManifestationID)11680303 035 $a(PQKBTitleCode)TC0001164708 035 $a(PQKBWorkID)11198265 035 $a(PQKB)11389707 035 $a(MiAaPQ)EBC1909044 035 $a(MiAaPQ)EBC5897784 035 $a(Au-PeEL)EBL5897784 035 $a(OCoLC)873818183 035 $a(PPN)185283810 035 $a(EXLCZ)993710000000077354 100 $a20191011d2014 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aNext-generation sequencing $ecurrent technologies and applications /$fedited by Jianping Xu 210 1$aNorfolk, England :$cCaister Academic Press,$d[2014] 210 4$dİ2014 215 $a1 online resource (173 p.) 300 $aDescription based upon print version of record. 311 $a1-908230-33-9 320 $aIncludes bibliographical references and index. 327 $aContents; Current books of interest; Contributors; Preface; 1; An Overview of Next-generation Genome Sequencing Platforms; Introduction; Second generation sequencing platforms; Third-generation sequencing platforms; Concluding remarks; 2: Attomole-level Genomics with Single-molecule Direct DNA, cDNA and RNA Sequencing Technologies; Introduction; Materials; Methods; 3: SNP Assessment on Draft Genomes from Next-generation Sequencing Data; Background; Single nucleotide polymorphisms (SNPs); SNP calling with one sample on draft genomes with ACCUSA 327 $aHead-to-head comparisons of sequenced samples with ACCUSA 2Conclusions; 4: Processing Large-scale Small RNA Datasets in Silico; Introduction; Library preparation and sequencing; Helper tools; Analysis tools; Visualization tools; Discussion; 5: Utility of High-throughput Sequence Data in Rare Variant Detection; What is a rare variant?; Why is variant detection needed?; Utility of non-HTS methods for minority and rare variant detection; Status of rare variant detection by analysis of HTS data; How much HTS data is needed to accurately detect rare variants? 327 $aTesting the feasibility of analysing HTS for rare SNP detectionSources of errors; Experimental validation of correction approaches; Conclusions; 6: Detecting Breakpoints of Insertions and Deletions from Paired-end Short Reads; Introduction; Pindel: a pattern growth method to identify precise breakpoints of indels and SVs; Performance on real data (NA18507); Recent developments; Further advances of split-read approaches; Conclusion and future perspectives; 7: Novel Insights from Re-sequencing of Human Exomes Through NGS; Introduction; The protocol; Exome capture platforms and kits 327 $aQuality control and performance evaluationBioinformatics analysis; Applications in human disease research; Perspective; 8: Insights on Plant Development Using NGS Technologies; Introduction; Use RNA-seq to dissect transcription at the cellular resolution; Use ChIP-seq to dissect transcriptional networks; Use ChIP-seq to analyse the epigenome; Conclusions and perspectives; 9: Next-generation Sequencing and the Future of Microbial Metagenomics; Introduction; Tracking microbial diversity; Applying omics technologies; Designing experiments; Modelling microbial diversity; Concluding remarks 327 $a10: Next-generation Sequencing, Metagenomes and the Human MicrobiomeIntroduction; Marker-specific microbial community surveys; Metagenomics - high-throughput shotgun (HTS sequencing) of microbial communities; Applications of metagenomics to the study of human health and disease; Beyond the omes - systems biology views onto the host-microbiome interactions; The new generation of cloud-based informatics solutions for next-generation sequencing; Conclusion; Index 330 $aHigh-throughput, next generation sequencing (NGS) technologies are capable of producing a huge amount of sequence data in a relatively short time and have revolutionized genome research in recent years. The powerful and flexible nature of NGS has made it an indispensable tool for a broad spectrum of biological sciences, and NGS technologies have transformed scientific research in many fields. Written by experts from around the world, this book explores the most recent advances in NGS instrumentation and data analysis. The book begins with a comprehensive description of current NGS platforms, t 606 $aBioinformatics 606 $aNucleotide sequence 606 $aSequence alignment (Bioinformatics) 606 $aSequential analysis 615 0$aBioinformatics. 615 0$aNucleotide sequence. 615 0$aSequence alignment (Bioinformatics) 615 0$aSequential analysis. 676 $a570.285 702 $aXu$b Jianping$c(Professor of Biology),$f1965- 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910828718203321 996 $aNext-generation sequencing$92039973 997 $aUNINA