LEADER 05185nam 2200709Ia 450 001 9910828689403321 005 20200520144314.0 010 $a1-281-18650-3 010 $a9786611186500 010 $a0-08-052819-8 035 $a(CKB)1000000000384305 035 $a(EBL)331913 035 $a(OCoLC)476132220 035 $a(SSID)ssj0000112459 035 $a(PQKBManifestationID)11129190 035 $a(PQKBTitleCode)TC0000112459 035 $a(PQKBWorkID)10086928 035 $a(PQKB)11664529 035 $a(Au-PeEL)EBL331913 035 $a(CaPaEBR)ebr10216739 035 $a(CaONFJC)MIL118650 035 $z(PPN)182572013 035 $a(PPN)170265250 035 $a(FR-PaCSA)41001493 035 $a(MiAaPQ)EBC331913 035 $a(EXLCZ)991000000000384305 100 $a19950908d1996 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aBiotechnology $ea laboratory course /$fJeffrey M. Becker, Guy A. Caldwell, Eve Ann Zachgo 205 $a2nd ed. 210 $aSan Diego $cAcademic Press$dc1996 215 $a1 online resource (283 p.) 300 $aDescription based upon print version of record. 311 $a0-12-084562-8 320 $aIncludes bibliographical references and index. 327 $aFront Cover; Biotechnology: A Laboratory Course; Copyright Page; Contents; Preface to the Second Edition; Preface to the First Edition; Acknowledgments; Suggested Schedule for Exercises; Introductory Notes: Record Keeping and Safety Rules; Format of Student Laboratory Records; The Ten Commandments of Record Keeping; Safety Rules in the Laboratory; Exercise 1. Aseptic Technique and Establishing Pure Cultures: The Streak Plate and Culture Transfer; Exercise 2. Preparation of Culture Media; Exercise 3. The Growth Curve; Exercise 4. Isolation of Plasmid DNA from Escherichia coli: The Mini-Prep 327 $aExercise 5. Purification, Concentration, and Quantitation of DNAExercise 6. Large-Scale Isolation of Plasmid DNA by Column Chromatography; Exercise 7. Amplification of a lacZ Gene Fragment by the Polymerase Chain Reaction; Exercise 8. Restriction Digestion and Agarose Gel Electrophoresis; Exercise 9. Southern Transfer; Exercise 10. Preparation, Purification, and Hybridization of Probe; Exercise 11. Transformation of Saccharomyces cerevisiae; Exercise 12. Isolation of Plasmid from Yeast and Escherichia coil Transformation; Exercise 13. Protein Assays 327 $aExercise 14. Qualitative Assay for b-Galactosidase in Yeast ColoniesExercise 15. Determination of b-Galactosidase in Permeabilized Yeast Cells; Exercise 16. Assay of b-Galactosidase in Cell Extracts; Exercise 17. b-Galactosidase Purification; Exercise 18. Western Blot: Probe of Protein Blot with Antibody to b-Galactosidase; Appendix 1. Alternative Protocols and Experiments; Exercise 1A Isolation and Characterization of Auxotrophic Yeast Mutants; Exercise 2A Measurement of pH; Exercise 3A Use of the Spectrophotometer; Exercise 6A Isolation of Plasmid DNA: The Maxi-Prep 327 $aExercise 10A Colony HybridizationAppendix 2. Buffer Solutions; Appendix 3. Preparation of Buffers and Solutions; Appendix 4. Properties of Some Common Concentrated Acids and Bases; Appendix 5. Use of Micropipettors; Appendix 6. Safe Handling of Microorganisms; Appendix 7. List of Cultures; Appendix 8. Storage of Cultures and DNA; Appendix 9. Sterilization Methods; Appendix 10. Preparation of Stock Solutions for Culture Media; Appendix 11. Growth in Liquid Medium; Appendix 12. Determination of Viable Cells; Appendix 13. Determination of Cell Mass; Appendix 14. Determination of Cell Number 327 $aAppendix 15. Nomenclature of StrainsAppendix 16. Glassware and Plasticware; Appendix 17. Preparation of Tris and EDTA; Appendix 18. Basic Rules for Handling Enzymes; Appendix 19. Effects of Common Contaminants on Protein Assays; Appendix 20. Manufacturers' and Distributors' Addresses; Appendix 21. Surfing the Bionet: World Wide Web Addresses; Glossary; Index 330 $aThe objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiae and Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures and 606 $aBiochemical engineering 606 $aBiotechnology$vLaboratory manuals 615 0$aBiochemical engineering. 615 0$aBiotechnology 676 $a660.6 676 $a660/.6/078 20 676 $a660.6078 700 $aBecker$b Jeffrey M$0621784 701 $aCaldwell$b Guy A$01635487 701 $aZachgo$b Eve Ann$01635488 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910828689403321 996 $aBiotechnology$93976298 997 $aUNINA