LEADER 04859oam 2200493 450 001 9910827166303321 005 20170523091546.0 010 $a0-12-382240-8 035 $a(OCoLC)854682481 035 $a(MiFhGG)GVRL8DOZ 035 $a(EXLCZ)992550000001106276 100 $a20140429d2013 uy 0 101 0 $aeng 135 $aurun|---uuuua 181 $ctxt 182 $cc 183 $acr 200 10$aBioconjugate techniques /$fGreg T. Hermanson, Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL 205 $a3rd ed. 210 $aLondon $cAcademic Press$dc2013 210 1$aLondon :$cAcademic Press,$d2013. 215 $a1 online resource (xvii, 1146 pages) $cillustrations (some color) 225 0 $aGale eBooks 300 $aDescription based upon print version of record. 311 $a0-12-382239-4 311 $a1-299-75383-3 320 $aIncludes bibliographical references and index. 327 $aFront Cover; Bioconjugate Techniques; Copyright Page; Dedication; Contents; Preface to the Third Edition; Acknowledgments; Health and Safety; Intellectual Property; Important Information; 1. Introduction to Bioconjugation; 1. What is Bioconjugation?; 2. Bioconjugation Strategy and Design; 2.1. Start with the Application in Mind; 2.2. Designing the Optimal Bioconjugate; 3. The Applications of Bioconjugates; 3.1. Assay and Quantification; Bioconjugates Used in Heterogeneous Immunoassays; Bioconjugates for Enzyme-Linked Immunoassays; Chemiluminescent Acridinium Ester Conjugates 327 $aFluorescent Oligonucleotide ProbesMolecular Scaffolds to Increase Assay Sensitivity; Bioconjugates Used in Homogeneous Assays; Bioconjugates for FRET Assays; Molecular Beacons and TaqMan Probes; Bioluminescence Resonance Energy Transfer (BRET); Protein Fragment Complementation Assays: Split Reporters; Mass Tags for Quantitative Mass Spectrometry; Limiting Nonspecificity in Bioconjugate Designs; 3.2. Detection, Tracking, and Imaging; Fluorescently Labeled Antibodies and Streptavidin; FRET Based Protease Probes; Interacting Proteins or Domains; Probing by Chemoselective Ligation 327 $aProximity Ligation AssaysStaining with Antibody-Enzyme Conjugates; Bioconjugates for Western Blot Detection; Bioconjugates for Super Resolution Microscopy; 3.3. Purification, Capture, and Scavenging; Purification using Immobilized Affinity Ligands; Immunoprecipitation Techniques; Affinity Capture of Post-Translational Modifications; Affinity Capture using Active Site Binding Probes; Affinity Capture of Recombinant Fusion Proteins; Covalent Fusion Tag Technology; Scavenging of Contaminants or Unwanted Components; 3.4. Catalysis and Chemical Modification 327 $aImmobilized Proteases for Proteomic AnalysisImmobilized Reactors in Bioengineering; 3.5. Therapeutics and in vivo Diagnostics; Bioconjugates for Cancer Therapy; Antibody Targeting for Biotherapeutics; Polymeric Scaffolds and Nanoparticles for Biotherapeutic Conjugates; Antibody-Directed Enzyme Prodrug Therapy; Radiolabeled Bioconjugates for Cancer; Boron Neutron Capture Therapy; Photodynamic Therapy; Diagnostic Bioconjugates for In Vivo Imaging; Bioconjugates for Radio Imaging; Bioconjugates for High-Contrast Imaging; NIR Fluorescent Conjugates for In Vivo Imaging 327 $a3.6. Vaccines and Immune ModulationCancer Vaccines; Immunogen Conjugates in the Production of Antibodies; 4. Summary; 2. Functional Targets for Bioconjugation; 1. Modification of Amino Acids, Peptides, and Proteins; 1.1. Protein Structure and Reactivity; Amino Acids; Nucleophilic Reactions and the pI of Amino Acid Side Chains; Secondary, Tertiary, and Quaternary Structure; Prosthetic Groups, Cofactors, and Post-Translational Modifications; Protecting the Native Conformation and Activity of Proteins; Oxidation of Amino Acids in Proteins and Peptides 327 $aSolvent Accessibility of Functional Targets in Proteins 330 $aBioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.