LEADER 05459nam 2200685Ia 450 001 9910826608003321 005 20200520144314.0 010 $a1-282-76003-3 010 $a9786612760037 010 $a1-84816-468-8 035 $a(CKB)2490000000001624 035 $a(EBL)1681510 035 $a(OCoLC)879025366 035 $a(SSID)ssj0000412325 035 $a(PQKBManifestationID)11913136 035 $a(PQKBTitleCode)TC0000412325 035 $a(PQKBWorkID)10367019 035 $a(PQKB)10426427 035 $a(MiAaPQ)EBC1681510 035 $a(WSP)00000501 035 $a(Au-PeEL)EBL1681510 035 $a(CaPaEBR)ebr10422341 035 $a(CaONFJC)MIL276003 035 $a(PPN)159179300 035 $a(EXLCZ)992490000000001624 100 $a20091020d2010 uy 0 101 0 $aeng 135 $aurbuu|||uu||| 181 $ctxt 182 $cc 183 $acr 200 10$aAtomic force microscopy for biologists /$fVictor J. Morris, Andrew .R Kirby, A. Patrick Gunning 205 $a2nd ed. 210 $aLondon $cImperial College Press$dc2010 215 $a1 online resource (423 p.) 300 $aDescription based upon print version of record. 311 $a1-84816-467-X 320 $aIncludes bibliographical references and index. 327 $aCONTENTS; Acknowledgements; CHAPTER 1 AN INTRODUCTION; CHAPTER 2 APPARATUS; 2.1. The atomic force microscope; 2.2. Piezoelectric scanners; 2.3. Probes and cantilevers; 2.3.1. Cantilever geometry; 2.3.2. Tip shape; 2.3.3. Tip functionality; 2.4. Sample holders; 2.4.1. Liquid cells; 2.5. Detection methods; 2.5.1. Optical detectors: laser beam deflection; 2.5.2. Optical detectors: interferometry; 2.5.3. Electrical detectors: electron tunneling; 2.5.4. Electrical detectors: capacitance; 2.5.5. Electrical detectors: piezoelectric cantilevers; 2.6. Control systems; 2.6.1. AFM electronics 327 $a2.6.2. Operation of the electronics 2.6.3. Feedback control loops; 2.6.4. Design limitations; 2.6.5. Enhancing the performance of large scanners; 2.7. Vibration isolation: thermal and mechanical; 2.8. Calibration; 2.8.1. Piezoelectric scanner non-linearity; 2.8.2. Tip related factors: convolution; 2.8.3. Calibration standards; 2.8.4. Tips for scanning a calibration specimen; 2.9. Integrated AFMs; 2.9.1. Combined AFM-light microscope (AFM-LM); 2.9.2. 'Submarine' AFM - the combined AFM - Langmuir Trough; 2.9.3. Combined AFM-surface plasmon resonance (AFM-SPR); 2.9.4. Cryo-AFM; References 327 $aUseful information sources CHAPTER 3 BASIC PRINCIPLES; 3.1. Forces; 3.1.1. The Van der Waals force and force-distance curves; 3.1.2. The electrostatic force; 3.1.3. Capillary and adhesive forces; 3.1.4. Double layer forces; 3.2. Imaging modes; 3.2.1. Contact dc mode; 3.2.2. Ac modes: Tapping and non-contact; Tapping in air; Tapping under liquid; True, non-contact ac mode; Tuning the cantilever; Influence of drive frequency; 3.2.3. Deflection mode; 3.3. Image types; 3.3.1. Topography; 3.3.2. Frictional force; 3.3.3. Phase; 3.4. Substrates; 3.4.1. Mica; 3.4.2. Glass; 3.4.3. Graphite 327 $a3.5. Common problems 3.5.1. Thermal drift; 3.5.2. Multiple tip effects; 3.5.3. The 'pool' artifact; 3.5.4. Optical interference on highly reflective samples; 3.5.5. Sample roughness; 3.5.6. Sample mobility; 3.5.7. Imaging under liquid; 3.6. Getting started; 3.6.1. DNA; 3.6.2. Troublesome large samples; 3.7. Image optimisation; 3.7.1. Grey levels and colour tables; 3.7.2. Brightness and contrast; 3.7.3. High and low pass filtering; 3.7.4. Normalisation and plane fitting; 3.7.5. Despike; 3.7.6. Fourier filtering; 3.7.7. Correlation averaging; 3.7.8. Stereographs and anaglyphs 327 $a3.7.9. Do your homework! References; CHAPTER 4 MACROMOLECULES; 4.1. Imaging methods; 4.1.1. Tip adhesion, molecular damage and displacement; 4.1.2. Depositing macromolecules onto substrates; 4.1.3. Metal coated samples; 4.1.4. Imaging in air; 4.1.5. Imaging under non-aqueous liquids; 4.1.6. Binding molecules to the substrate; 4.1.7. Imaging under water or buffers; 4.2. Nucleic acids: DNA; 4.2.1. Imaging DNA; 4.2.2. DNA conformation, size and shape; 4.2.3. DNA-protein interactions; 4.2.4. Location and mapping of specific sites; 4.2.5. Chromosomes; 4.3. Nucleic acids: RNA; 4.4. Polysaccharides 327 $a4.4.1. Imaging polysaccharides 330 $aAtomic force microscopy (AFM) is part of a range of emerging microscopic methods for biologists which offer the magnification range of both the light and electron microscope, but allow imaging under the ""natural"" conditions usually associated with the light microscope. To biologists, AFM offers the prospect of high resolution images of biological material, images of molecules and their interactions even under physiological conditions, and the study of molecular processes in living systems. This book provides a realistic appreciation of the advantages and limitations of the technique and the 606 $aAtomic force microscopy 606 $aBiology$xTechnique 615 0$aAtomic force microscopy. 615 0$aBiology$xTechnique. 676 $a570.282 700 $aMorris$b V. J$0623296 701 $aKirby$b A. R$01666679 701 $aGunning$b A. P$01666680 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910826608003321 996 $aAtomic force microscopy for biologists$94026059 997 $aUNINA