LEADER 05285nam 2200673 450 001 9910144117903321 005 20170809154544.0 010 $a1-281-84239-7 010 $a9786611842390 010 $a3-527-61253-X 010 $a3-527-61252-1 035 $a(CKB)1000000000555626 035 $a(EBL)482019 035 $a(OCoLC)291090796 035 $a(SSID)ssj0000104258 035 $a(PQKBManifestationID)11131019 035 $a(PQKBTitleCode)TC0000104258 035 $a(PQKBWorkID)10078050 035 $a(PQKB)10445682 035 $a(MiAaPQ)EBC482019 035 $a(EXLCZ)991000000000555626 100 $a20160819h19971997 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aAntisense and ribozyme methodology /$fIan Gibson, editor 210 1$aLondon, [England] :$cChapman & Hall,$d1997. 210 4$dİ1997 215 $a1 online resource (96 p.) 225 1 $aLaboratory Companion Series 300 $aIncludes index. 300 $a"With 10 Figures, some in Color"--Title page. 311 $a3-8261-0079-4 311 $a3-527-30889-X 327 $aAntisense and Ribozyme Methodology; Contents; CHAPTER 1. Antisense and Ribozyme Methodology; 1.1 The Potential; 1.2 Antisense Technology; 1.2.1 Problems; 1.2.2 Resistance to Nucleases; 1.2.3 Entry into Cells; 1.2.4 How Antisense Works; 1.2.5 Success; 1.3 Ribozymes; 1.3.1 What Are They?; 1.3.2 Problems; 1.3.3 Stable Ribozymes; 1.3.4 Designing Ribozymes; 1.4 Ribozymes or Antisense DNAs?; 1.5 The Choice Today!!; CHAPTER 2. Design and Synthesis of Antisense DNA Molecules; 2.1 Introduction; 2.2 Synthesis of Methylphosphonodiester-Phosphodiester Chimeric Oligodeoxynucleotides 327 $a2.2.1 Materials and Chemicals2.2.2 Solutions; 2.2.3 Maximizing Product Purity; 2.2.4 Deprotection of Chimeric Oligodeoxynucleotides; 2.2.5 Failed Sequences; 2.3 Primary Purification by Reversed-Phase, Solid-Phase Extraction on C18 SEP-PAK Cartridges; 2.3.1 Equipment; 2.3.2 Method; 2.3.3 Purification of the Oligodeoxynucleotide; 2.3.4 Further Purification; 2.4 Analysis and Purification by HPLC; 2.4.1 Analysis of Chimeric Oligodeoxynucleotides by HPLC; 2.4.2 Purification of Chimeric Oligodeoxynucleotides by HPLC; 2.4.3 Re-Use of Columns 327 $a2.5 Synthesis of Chimeric Oligodeoxynucleotides with Fluorescein Attached2.6 Summary; CHAPTER 3. The Design and Synthesis of Hammerhead Ribozymes; 3.1 Introduction; 3.2 The Design of Hammerhead Ribozymes; 3.3 Improving the Reactions; 3.3.1 Accessibility of the Target - Substrate Binding; 3.3.2 Finding the Target; 3.3.3 Theoretical Considerations; 3.3.4 Experimental Approaches; 3.3.5 Kinetic Studies; 3.4 Length of Arms; 3.4.1 Choosing Antisense Arms of Hammerhead Ribozymes; 3.4.2 Arms of Different Lengths; 3.5 Cleavage of the Target Motif; 3.6 Synthesis of Ribozymes 327 $a3.6.1 Chemical Synthesis of Short Hammerhead Ribozymes3.6.2 Enzymatic Transcription in Vitro; 3.7 Endogenous Expression of Ribozyme Genes; CHAPTER 4. Delivery of Ribozymes and Antisense DNA Molecules into Mammalian Cells; 4.1 Introduction; 4.2 Exogenous Application; 4.2.1 lntracytoplasmic Delivery of Antisense Oligodeoxynucleotides by Reversible Plasma Membrane Permeabilization with Streptolysin O; 4.3 Microinjection; 4.4 Other Methods Used in Nucleic Acid Transfection; 4.5 Electroporation; 4.5.1 Method; 4.5.2 Transfection: Optimization of Conditions 327 $a4.5.3 Mechanism of Uptake Following Electroporation4.5.4 Benefits and Drawbacks of Electrophoretic-Mediated Uptake; 4.6 Diethylaminoethyl-Dextran (DEAE- Dextran) and DNA Transfection; 4.6.1 Methods for Transfection of Adherent Cells; 4.6.2 Possible Alterations of the Above Protocol; 4.6.3 Transfection of Cells Growing in Suspension; 4.6.4 Transfection Optimization; 4.6.5 Distribution Mechanism of DEAE-Dextran Uptake and lntracellular; 4.6.6 Benefits and Drawbacks; 4.7 Calcium Phosphate Transfection; 4.7.1 Method; 4.7.2 Possible Alterations to Above Method; 4.7.3 Method Optimization 327 $a4.7.4 Calcium Phosphate-Mediated Uptake and lntracellular Distribution 330 $aAntisense and ribozymes have a relatively short yet successful history as research tools in gene expression studies, and thus are considered as having high potential reagents in treating viral infections and cancer.This laboratory companion provides detailed information on the potential, advantages and limitations of this methodology. It critically discusses potential pitfalls, presents strategies for choosing targets and delivery systems, so as to allow the selection of the optimum methodology for achieving fast and reliable experimental success with any human or other biological system. 410 0$aLaboratory companion. 606 $aAntisense nucleic acids$vLaboratory manuals 606 $aCatalytic RNA$vLaboratory manuals 608 $aElectronic books. 615 0$aAntisense nucleic acids 615 0$aCatalytic RNA 676 $a572.88 676 $a615.36 702 $aGibson$b Ian$f1938- 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910144117903321 996 $aAntisense and ribozyme methodology$91933177 997 $aUNINA LEADER 04865nam 2200805 450 001 9910826393903321 005 20230125181419.0 010 $a1-60649-547-X 035 $a(CKB)2550000001113731 035 $a(EBL)1365264 035 $a(OCoLC)857365402 035 $a(SSID)ssj0001141689 035 $a(PQKBManifestationID)12501277 035 $a(PQKBTitleCode)TC0001141689 035 $a(PQKBWorkID)11090871 035 $a(PQKB)11114594 035 $a(OCoLC)859338093 035 $a(CaBNVSL)swl00402758 035 $a(Au-PeEL)EBL1365264 035 $a(CaPaEBR)ebr10767928 035 $a(CaONFJC)MIL513586 035 $a(CaSebORM)9781606495469 035 $a(MiAaPQ)EBC1365264 035 $a(EXLCZ)992550000001113731 100 $a20130927d2013 fy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aEducating for values-driven leadership $egiving voice to values across the curriculum /$fMary C. Gentile 205 $aFirst edition. 210 1$aNew York, New York (222 East 46th Street, New York, NY 10017) :$cBusiness Expert Press,$d2013. 215 $a1 online resource (228 p.) 225 1 $aPrinciples of responsible management education (PRME) collection 300 $aPart of: 2013 digital library. 311 $a1-60649-546-1 311 $a1-299-82335-1 320 $aIncludes bibliographical references (pages 203-212) and index. 327 $aPart 1. Introduction to giving voice to values -- 1. Educating for values-driven leadership: giving voice to values across the curriculum / Mary C. Gentile -- Part 2. GVV across the curriculum -- 2. Giving voice to values in the economics classroom / Daniel G. Arce -- 3. Teaching change leadership for sustainable business: strategies from the "giving voice to values" curriculum / Christopher P. Adkins -- 4. Giving voice to values in accounting education / Steven M. Mintz and Roselyn E. Morris -- 5. Giving voice to values in human resource management practice and education / Charmine E. J. Ha?rtel and Amanda Roan -- 6. Giving voice to values for the public sector: an exploratory approach / Kenneth Wiltshire and Stephen Jones -- 7. Developing negotiation skills through the giving voice to values scripting approach / Melissa Manwaring -- 8. The ethics of voicing one's values / Leigh Hafrey -- 9. Voicing values in pursuit of a social mission: the role of giving voice to values in social entrepreneurship teaching / Denise Crossan -- 10. Applying the giving voice to values framework to address leadership dilemmas: experiences in an Indian executive MBA program / Ranjini Swamy -- 11. Giving voice to values in operations management / Kathleen E. McKone-Sweet -- 12. Voicing values in marketing education: Indian perspectives / Subhasis Ray -- 13. Giving voice to values and ethics across the curriculum at the United States Air Force Academy / Claudia J. Ferrante, Patrick E. Heflin, and David A. Levy -- Notes -- References -- Index. 330 3 $aDespite four decades of good faith effort to teach Ethics in business schools, readers of the business press are still greeted on a regular basis with headlines about egregious excess and scandal. It becomes reasonable to ask why these efforts have not been working. Business faculty in ethics courses spend a lot of time teaching theories of ethical reasoning and analyzing those big, thorny dilemmas--triggering what one professor called "ethics fatigue." Some students find such approaches intellectually engaging; others find them tedious and irrelevant. Either way, sometimes all they learn is how to frame the case to justify virtually any position, no matter how cynical or self-serving. Utilitarianism, after all, is tailor-made for a free market economy. 410 0$a2013 digital library. 410 0$aPrinciples of responsible management education (PRME) collection. 606 $aBusiness education 606 $aBusiness ethics 606 $aSocial responsibility of business 610 $abusiness ethics 610 $amanagement education 610 $abusiness education 610 $apedagogy 610 $avalues 610 $avalues-driven leadership 610 $across-functional education 610 $aenvironmental and social impacts management 610 $acorporate social responsibility 610 $asustainability 610 $aCSR communication 610 $astakeholder communication 615 0$aBusiness education. 615 0$aBusiness ethics. 615 0$aSocial responsibility of business. 676 $a650.0711 700 $aGentile$b Mary C.$0116477 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910826393903321 996 $aEducating for values-driven leadership$94029647 997 $aUNINA