LEADER 05186nam 2200589 450 001 9910826143903321 005 20230828162620.0 010 $a0-12-800012-0 010 $a0-12-800788-5 035 $a(CKB)2560000000301461 035 $a(EBL)1873130 035 $a(SSID)ssj0001548491 035 $a(PQKBManifestationID)16151620 035 $a(PQKBTitleCode)TC0001548491 035 $a(PQKBWorkID)14802236 035 $a(PQKB)11012911 035 $a(MiAaPQ)EBC1873130 035 $a(Au-PeEL)EBL1873130 035 $a(CaPaEBR)ebr10992157 035 $a(CaONFJC)MIL785192 035 $a(OCoLC)897115961 035 $a(EXLCZ)992560000000301461 100 $a20141210h20142014 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aAdvances in protein chemistry and structural biology$hVolume ninety seven $emetal-containing enzymes /$fedited by Christo Z. Christov 210 1$aWaltham, Massachusetts ;$aSan Diego, California :$cAcademic Press,$d2014. 210 4$dİ2014 215 $a1 online resource (174 p.) 300 $aDescription based upon print version of record. 320 $aIncludes bibliographical references and indexes. 327 $aFront Cover; Metal-Containing Enzymes; Copyright; Contents; Contributors; Preface; Chapter 1: Type-3 Copper Proteins: Recent Advances on Polyphenol Oxidases; 1. Introduction; 2. Primary Structure and Molecular Weights of PPOs; 2.1. General sequence structure; 2.2. Core domain, C-terminal domain, and its proteolytic cleavage site; 2.3. Conserved amino acid motifs in the core domain; 2.4. Conserved amino acid motifs in the C-terminal domain; 2.5. Transit peptide and location of PPOs; 2.6. Sequence homologies within PPOs; 2.7. In vitro activation of PPOs; 2.8. In vivo activation of PPOs 327 $a2.9. Mutants of PPOs2.9.1. Mutants of plant PPOs; 2.9.2. Mutants of fungal PPOs; 2.9.3. Mutants of bacterial PPOs; 3. Oxo Complex; 3.1. Oxo complex investigated by UV/vis absorption spectroscopy; 3.2. Oxo complex investigated by CD spectroscopy; 3.3. Oxo complex investigated by resonance Raman spectroscopy; 3.4. Oxo complex of catechol oxidase; 3.5. Oxo complex of tyrosinase; 3.6. Oxo complex of aureusidin synthase; 3.7. Oxo complex of hemocyanin; 4. X-ray Crystallographic Structural Data of PPOs and Hemocyanins; 4.1. Published structures in the protein data bank 327 $a4.1.1. Published structures in the PDB of hemocyanins4.1.2. Published structures in the PDB of catechol oxidases; 4.1.3. Published structures in the PDB of tyrosinases; 4.2. Structural differences of tyrosinases and catechol oxidases; 5. Conclusions and Outlook; Acknowledgments; References; Chapter 2: Biophysical Studies of Matrix Metalloproteinase/Triple-Helix Complexes; 1. MMPs and Collagen Hydrolysis; 2. Structures of Full-Length, Collagenolytic MMPs in Solution and in the Solid State; 3. Structural Evaluation of MMP Interactions with Collagen; 4. Mechanism of Collagenolysis 327 $a5. Heterogeneity in MMP StructuresAcknowledgment; References; Chapter 3: Catalytic Mechanisms of Metallohydrolases Containing Two Metal Ions; 1. Introduction; 2. Metallo-?-Lactamases, Major Culprits in the Emergence of Antibiotic Resistance; 3. Methionine Aminopeptidase, a Target for Novel Anticancer Drugs; 4. Glycerophosphodiesterase, a Very Promiscuous Potential Bioremediator; 5. PAP, an Alternative Target to Treat Osteoporosis; 6. Conclusions and Outlook: Agmatinase, an Emerging Target for Biotechnological Applications; Acknowledgments; References 327 $aChapter 4: Applications of Quantum Mechanical/Molecular Mechanical Methods to the Chemical Insertion Step of DNA and RNA ...1. Introduction; 2. Methods for Describing Reactive Pathways; 3. DNA Polymerase ?; 3.1. Abashkin, Erickson, and Burt (2001): A cluster QM calculation at steps along a proposed reaction path; 3.2. Rittenhouse, Apostoluk, Miller, and Straatsma (2003): A cluster QM calculation on the prechemistry complex of Pol ?; 3.3. Sawaya et al., 1997); 3.4. Batra et al., 2006); 3.5. Batra et al., 2008) 327 $a3.6. Batra et al. (2013): A QM/MM study based on the X-ray crystal structure of the Pol ? variant D256E 330 $aPublished continuously since 1944, the Advances in Protein Chemistry and Structural Biology series is the essential resource for protein chemists. Each volume brings forth new information about protocols and analysis of proteins. Each thematically organized volume is guest edited by leading experts in a broad range of protein-related topics. Describes advances in metal-containing enzymesChapters are written by authorities in their fieldTargeted to a wide audience of researchers, specialists, and studentsThe information provided in the volume is well supported by a number of high quality illus 606 $aProteins 615 0$aProteins. 676 $a572.7 702 $aChristov$b Christo 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910826143903321 996 $aAdvances in protein chemistry and structural biology$91495463 997 $aUNINA