LEADER 05468nam 2200673 450 001 9910826121503321 005 20200520144314.0 010 $a3-527-67341-5 010 $a3-527-67342-3 010 $a3-527-67339-3 035 $a(CKB)3710000000355746 035 $a(EBL)1953329 035 $a(OCoLC)903357111 035 $a(SSID)ssj0001467518 035 $a(PQKBManifestationID)11872175 035 $a(PQKBTitleCode)TC0001467518 035 $a(PQKBWorkID)11518381 035 $a(PQKB)10430308 035 $a(MiAaPQ)EBC1953329 035 $a(Au-PeEL)EBL1953329 035 $a(CaPaEBR)ebr11019995 035 $a(CaONFJC)MIL729454 035 $a(PPN)19558290X 035 $a(EXLCZ)993710000000355746 100 $a20150226h20152015 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aAnalyzing biomolecular interactions by mass spectrometry /$fedited by Jeroen Kool and Wilfried M. A. Niessen 210 1$aWeinheim, Germany :$cWiley-VCH Verlag GmbH & Co.,$d2015. 210 4$dİ2015 215 $a1 online resource (403 p.) 300 $aDescription based upon print version of record. 311 $a1-322-98172-8 311 $a3-527-33464-5 320 $aIncludes bibliographical references and index. 327 $a""Analyzing Biomolecular Interactions by Mass Spectrometry""; ""Contents ""; ""List of Contributors ""; ""Preface ""; ""Abbreviations ""; ""Chapter 1 Introduction to Mass Spectrometry, a Tutorial ""; ""1.1 Introduction ""; ""1.2 Figures of Merit ""; ""1.2.1 Introduction ""; ""1.2.2 Resolution ""; ""1.2.3 Mass Accuracy "" 327 $a""1.2.4 General Data Acquisition in MS """"1.3 Analyte Ionization ""; ""1.3.1 Introduction ""; ""1.3.2 Electrospray Ionization ""; ""1.3.3 Matrix-Assisted Laser Desorption Ionization ""; ""1.3.4 Other Ionization Methods ""; ""1.3.5 Solvent and Sample Compatibility Issues "" 327 $a""1.4 Mass Analyzer Building Blocks """"1.4.1 Introduction ""; ""1.4.2 Quadrupole Mass Analyzer ""; ""1.4.3 Ion-Trap Mass Analyzer ""; ""1.4.4 Time-of-Flight Mass Analyzer ""; ""1.4.5 Fourier Transform Ion Cyclotron Resonance Mass Spectrometer ""; ""1.4.6 Orbitrap Mass Analyzer "" 327 $a""1.4.7 Ion Detection """"1.5 Tandem Mass Spectrometry ""; ""1.5.1 Introduction: ""Tandem-in-Time"" and ""Tandem-in-Space"" ""; ""1.5.2 Ion Dissociation Techniques ""; ""1.5.3 Tandem Quadrupole MS-MS Instruments ""; ""1.5.4 Ion-Trap MS n Instruments ""; ""1.5.5 Tandem TOF (TOF-TOF) Instruments "" 327 $a""1.5.6 Hybrid Instruments (Q-TOF, Q-LIT, IT-TOF) """"1.5.7 MS-MS and MSn in FT-ICR-MS ""; ""1.5.8 Orbitrap-Based Hybrid Systems ""; ""1.5.9 Ion-Mobility Spectrometry-Mass Spectrometry ""; ""1.6 Data Interpretation and Analytical Strategies ""; ""1.6.1 Data Acquisition in MS Revisited "" 327 $a""1.6.2 Quantitative Bioanalysis and Residue Analysis "" 330 $aThis monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomi 606 $aBiomolecules$xAnalysis 606 $aChemistry, Analytic$xQuantitative 615 0$aBiomolecules$xAnalysis. 615 0$aChemistry, Analytic$xQuantitative. 676 $a572.33 702 $aKool$b Jeroen 702 $aNiessen$b Wilfried M. A. 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910826121503321 996 $aAnalyzing biomolecular interactions by mass spectrometry$93960960 997 $aUNINA