LEADER 05680nam 2200745Ia 450 001 9910824873503321 005 20240516061106.0 010 $a1-283-39799-4 010 $a9786613397997 010 $a1-118-10214-2 010 $a1-118-10213-4 010 $a1-118-10216-9 035 $a(CKB)2670000000133138 035 $a(EBL)693197 035 $a(OCoLC)769341474 035 $a(SSID)ssj0000576242 035 $a(PQKBManifestationID)11330606 035 $a(PQKBTitleCode)TC0000576242 035 $a(PQKBWorkID)10554107 035 $a(PQKB)11684748 035 $a(MiAaPQ)EBC693197 035 $a(Au-PeEL)EBL693197 035 $a(CaPaEBR)ebr10523267 035 $a(CaONFJC)MIL339799 035 $a(PPN)178708542 035 $a(EXLCZ)992670000000133138 100 $a20110412d2011 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aZebrafish $emethods for assessing drug safety and toxicity /$fedited by Patricia McGrath 205 $a1st ed. 210 $aHoboken, N.J. $cJohn Wiley & Sons$d2011 215 $a1 online resource (362 p.) 300 $aDescription based upon print version of record. 311 $a0-470-42513-X 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aZebrafish: Methods for Assessing Drug Safety and Toxicity; Contents; Preface; Contributors; Acknowledgments; 1. The Reproductive Biology and Spawning of Zebrafish in Laboratory Settings; 1.1 Introduction; 1.2 Overview of Zebrafish Reproductive Biology and Behavior; 1.3 Spawning Techniques and Technology; 1.4 Determining Factors for Reproduction in Laboratory Stocks of Zebrafish; 1.5 Conclusions; References; 2. Developmental Toxicity Assessment in Zebrafish; 2.1 Introduction; 2.2 Methods; 2.3 Results; 2.4 Discussion; References; 3. Use of Emerging Models for Developmental Toxicity Testing 327 $a3.1 Importance of Assessing Developmental Toxicity3.2 Current Methods for Assessing Developmental Toxicity; 3.3 Use of Emerging Models for Developmental Toxicity Testing; 3.4 New Guidelines for Chemical Testing Using Zebrafish; 3.5 Conclusions; References; 4. Assessment of Drug-Induced Cardiotoxicity in Zebrafish; 4.1 Introduction; 4.2 Zebrafish Heart; 4.3 Summary of Cardiotoxicity Study Design and Results; 4.4 Materials and Methods; 4.5 Results; 4.6 Conclusions; References; 5. Cardiotoxicity Studies in Zebrafish; 5.1 Introduction; 5.2 Repolarization Toxicity 327 $a5.3 Initial Screening: Bradycardia5.4 High-Resolution Assays of Repolarization; 5.5 Future Directions; References; 6. In Vivo Recording of the Adult Zebrafish Electrocardiogram; 6.1 Introduction; 6.2 Optimization of Zebrafish Electrocardiogram Recording; 6.3 Basic Intervals; 6.4 Drug Effects; 6.5 Conclusions; References; 7. Hematopoietic and Vascular System Toxicity; 7.1 Introduction; 7.2 Hematopoiesis and Vascular Development in the Zebrafish; 7.3 Morphological and Functional Assays to Assess Toxicity; 7.4 Summary; Acknowledgment; References; 8. Hepatotoxicity Testing in Larval Zebrafish 327 $a8.1 Introduction: The Larval Zebrafish Model8.2 Liver Development; 8.3 Hepatic Gene Knockdown and Mutation; 8.4 Hepatotoxicity Testing in Drug Discovery; 8.5 Phenotypic-Based Larval Zebrafish Hepatotoxicity Screens; 8.6 Secondary and Mechanistic Liver Assays; 8.7 Conclusions; References; 9. Whole Zebrafish Cytochrome P450 Assay for Assessing Drug Metabolism and Safety; 9.1 Introduction; 9.2 Background and Significance; 9.3 Materials and Methods; 9.4 Results; 9.5 Conclusions; Acknowledgment; References; 10. Methods for Assessing Neurotoxicity in Zebrafish; 10.1 Introduction 327 $a10.2 Limitations of Current Neurotoxicity Testing10.3 Assessing Neurotoxicity in Zebrafish; 10.4 Summary; Acknowledgments; References; 11. Zebrafish: A Predictive Model for Assessing Cancer Drug-Induced Organ Toxicity; 11.1 Introduction; 11.2 Materials and Methods; 11.3 Results; 11.4 Conclusions; Reference; 12. Locomotion and Behavioral Toxicity in Larval Zebrafish: Background, Methods, and Data; 12.1 Introduction; 12.2 Background; 12.3 Locomotion; 12.4 Zebrafish Models; 12.5 Analyzing Larval Locomotion; 12.6 Chemical Effects on Larval Locomotion; 12.7 Conclusions; Acknowledgments; References 327 $a13. Zebrafish: A Predictive Model for Assessing Seizure Liability 330 $aZebrafish: Methods for Assessing Drug Safety and Toxicity offers a practical guide for using zebrafish as a tool for toxicology studies. Consolidating key protocols and approaches to help researchers navigate the important and evolving field of zebrafish models for toxicity screening, this new title describes the methods for using the zebrafish as a model organism to assess compound-induced toxicity on all major organs. Individual chapters that concentrate on assays for each organ system are included and various analytical tools including microscopy, microplate readers, high content 606 $aLogperch$xGenetics 606 $aDrugs$xSafety measures 606 $aToxicology$xAnimal models 606 $aFish as laboratory animals 606 $aAnimal models in research 615 0$aLogperch$xGenetics. 615 0$aDrugs$xSafety measures. 615 0$aToxicology$xAnimal models. 615 0$aFish as laboratory animals. 615 0$aAnimal models in research. 676 $a597/.482 701 $aMcGrath$b Patricia$f1949-$01680310 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910824873503321 996 $aZebrafish$94048968 997 $aUNINA