LEADER 06059nam 2200697Ia 450 001 9910824534803321 005 20200520144314.0 010 $a1-5231-4630-3 010 $a1-60807-012-3 035 $a(CKB)2670000000044609 035 $a(EBL)946536 035 $a(OCoLC)796382978 035 $a(SSID)ssj0000423085 035 $a(PQKBManifestationID)11281847 035 $a(PQKBTitleCode)TC0000423085 035 $a(PQKBWorkID)10440496 035 $a(PQKB)11766329 035 $a(Au-PeEL)EBL946536 035 $a(CaPaEBR)ebr10412731 035 $a(CaBNVSL)mat09100412 035 $a(IEEE)9100412 035 $a(MiAaPQ)EBC946536 035 $a(EXLCZ)992670000000044609 100 $a20100702d2010 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aMethods in bioengineering $ealternative technologies to animal testing /$fTim Maguire, Eric Novik, editors 205 $a1st ed. 210 $aBoston, Mass. $cArtech House$dc2010 215 $a1 online resource (298 p.) 225 1 $aMethods in bioengineering series 300 $aDescription based upon print version of record. 311 $a1-60807-011-5 320 $aIncludes bibliographical references and index. 327 $aContents; Preface; Chapter 1 Current Methods for Prediction of Human Hepatic Clearance Using In Vitro Intrinsic Clearance; 1.1 Introduction; 1.2 Materials; 1.3 Methods; 1.3.1 Thawing the hepatocytes; 1.3.2 Clearance study using a hepatocyte suspension; 1.3.3 Clearance study using a plated hepatocyte culture; 1.3.4 Clearance study using a plated hepatocyte culture under a flow condition; 1.3.5 Sampling for the clearance study; 1.3.6 Sample analysis using LC-MS/MS; 1.4 Data Acquisition, Anticipated Results, and Interpretation; 1.4.1 Hepatocyte suspension and plated hepatocyte system 327 $a1.4.2 Physiologically based microfluidic systems1.5 Discussion and Commentary; 1.5.1 Hepatocyte suspension system; 1.5.2 Plated hepatocyte system; 1.5.3 Physiologically based microfluidic systems; 1.6 Summary; References; Chapter 2 Use of Permeability from Cultured Cell Linesand PAMPA System and Absorption from Experimental Animals for the Prediction of Absorption in Humans; 2.1 Introduction; 2.2 Materials; 2.3 Methods; 2.3.1 Cultured cell system; 2.3.2 PAMPA system; 2.3.3 In vivo absorption measurements; 2.4 Data Acquisition, Anticipated Results, and Interpretation; 2.4.1 Data analysis 327 $a2.4.2 Results and interpretation2.5 Discussion and Commentary; 2.5.1 Cell culture and PAMPA systems; 2.5.2 Absorption in experimental animals; 2.5.3 Rats; 2.5.4 Dogs; 2.5.5 Monkeys; 2.6 Summary; References; Chapter 3 Aggregating Brain Cell Cultures for Neurotoxicity Tests; 3.1 Introduction; 3.2 Experimental Design; 3.3 Materials; 3.3.1 Animals; 3.3.2 Special equipment; 3.3.3 Reagents; 3.3.4 Preparation of solutions and media; 3.4 Methods; 3.4.1 Washing and sterilizing the glassware; 3.4.2 Cell isolation and culture preparation 327 $a3.4.3 Maintenance of aggregating brain cell cultures (media replenishmentand subdivision)3.4.4 Preparation and treatment of replicate cultures; 3.4.5 Harvest of replicate cultures for various analytical procedures; 3.4.6 Examples of sample preparation and use for various analytical procedures; 3.4.7 Data Analysis; 3.5 Anticipated Results; 3.6 Discussion and Commentary; 3.7 Application Notes; 3.8 Summary Points; Acknowledgments; References; Chapter 4 Approaches Towards a Multiscale Model of Systemic Inflammation in Humans; 4.1 Introduction; 4.2 Materials 327 $a4.2.1 Human endotoxin model and data collection4.3 Methods; 4.3.1 Transcriptional dynamics and intrinsic responses; 4.3.2 Modeling inflammation at the cellular level; 4.3.3 Modeling inflammation at the systemic level; 4.4 Results; 4.4.1 Elements of the multiscale host response model of human inflammation; 4.4.2 Estimation of relevant model parameters; 4.4.3 Qualitative assessment of the model; 4.5 Conclusions; Acknowledgments; References; Chapter 5 A Liposome Assay for Evaluating the Ocular Toxicity of Chemicals; 5.1 Introduction; 5.2 Experimental Design; 5.3 Materials; 5.4 Methods 327 $a5.4.1 Preparation of calcein-loaded liposomes 330 3 $aWritten and edited by recognized experts in the field, the new Artech House Methods in Bioengineering book series offers detailed guidance on authoritative methods for addressing specific bioengineering challenges. Offering a highly practical presentation of each topic, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. Providing alternatives to animal testing is one of the hottest topics in biomedical research, and this groundbreaking volume addresses this critical issues head on. This unique book presents techniques and methods at the forefront of scientific research that have the potential to replace certain whole animal tests. Moreover, this book provides a platform where other widely accepted techniques and scientific advancements can be collated into a concise set of methods that can be implemented within both academic and industrial communities.$cPublisher abstract. 410 0$aArtech House methods in bioengineering series. 606 $aAnimal experimentation$xMoral and ethical aspects 606 $aAnimal welfare$xMoral and ethical aspects 606 $aHuman-animal relationships$xMoral and ethical aspects 615 0$aAnimal experimentation$xMoral and ethical aspects. 615 0$aAnimal welfare$xMoral and ethical aspects. 615 0$aHuman-animal relationships$xMoral and ethical aspects. 676 $a179.4 701 $aMaguire$b Timothy J$g(Timothy John)$01719973 701 $aNovik$b Eric$01719974 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910824534803321 996 $aMethods in bioengineering$94118239 997 $aUNINA