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024 7 $a10.1093/oso/9780199638734.001.0001
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100   $a20040402d2004      uy         0
101 0 $aeng
135   $aur|n|---|||||
181   $ctxt
182   $cc
183   $acr
200 00$aPhage display $ea practical approach /$fedited by Tim Clackson, Henry B. Lowman
205   $a1st ed.
210   $aOxford ;$aNew York $cOxford University Press$dc2004
215   $a1 online resource (357 p.)
225 1 $aPractical approach series ;$vno. 266
300   $aPreviously issued in print: 2004.
311   $a0-19-963873-X 
311   $a0-19-963874-8 
320   $aIncludes bibliographical references and index.
327   $aContents; Protocol list; List of abbreviations; List of contributors; 1 Introduction to phage biology and phage display; 2 Constructing phage display libraries by oligonucleotide-directed mutagenesis; 3 In vitro DNA recombination; 4 Phage selection strategies for improved affinity and specificity of proteins and peptides; 5 Rapid screening of phage displayed protein binding affinities by phage ELISA; 6 Identification of novel ligands for receptors using recombinant peptide libraries; 7 Substrate phage display
327   $a8 Protease-based selection of stably folded proteins and protein domains from phage display libraries9 Phage display of zinc fingers and other nucleic acid-binding motifs; 10 In vivo and ex vivo selections using phage-displayed libraries; 11 Screening phage libraries with sera; 12 Interaction cloning using cDNA libraries displayed on phage; 13 Phage antibody libraries; 14 Affinity maturation of phage antibodies; Appendix: Non-standard suppliers; Index; 
330 8 $aPhage display has become established as a powerful protein engineering method for identifying polypeptides with novel properties, and altering the properties of existing ones. Although the technique is widely used in biological research and drug discovery, it remains technically challenging, and new applications and procedures continue to evolve. Phage Display - A Practical Approach is an up-to-date, comprehensive and integrated experimental guide to the technique, useful for novice and expert alike. The book aims to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analysing the results.
410  0$aPractical approach series ;$v266.
606   $aBacteriophages
606   $aViral proteins
606   $aMicrobial biotechnology
615  0$aBacteriophages.
615  0$aViral proteins.
615  0$aMicrobial biotechnology.
676   $a579.26
676   $a579.26
701   $aClackson$b Tim$01694499
701   $aLowman$b Henry B$01694500
801  0$bMiAaPQ
801  1$bMiAaPQ
801  2$bMiAaPQ
906   $aBOOK
912   $a9910824041603321
996   $aPhage display$94073128
997   $aUNINA