LEADER 05612nam 2200745 450 001 9910823859903321 005 20200520144314.0 010 $a1-118-89089-2 010 $a1-118-89083-3 010 $a1-118-89090-6 035 $a(CKB)3710000000113265 035 $a(EBL)1690924 035 $a(SSID)ssj0001193900 035 $a(PQKBManifestationID)11681730 035 $a(PQKBTitleCode)TC0001193900 035 $a(PQKBWorkID)11165402 035 $a(PQKB)11641575 035 $a(OCoLC)865452420 035 $a(MiAaPQ)EBC1690924 035 $a(DLC) 2013049612 035 $a(Au-PeEL)EBL1690924 035 $a(CaPaEBR)ebr10870606 035 $a(CaONFJC)MIL611514 035 $a(PPN)22157252X 035 $a(EXLCZ)993710000000113265 100 $a20140522h20142014 uy 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt 182 $cc 183 $acr 200 00$aDried blood spots $eapplications and techniques /$fedited by Wenkui Li, Mike S. Lee 210 1$aHoboken, New Jersey :$cWiley,$d2014. 210 4$dİ2014 215 $a1 online resource (377 p.) 225 1 $aWiley Series on Pharmaceutical Science and Biotechnology : Practices, Applications and Methods 300 $aIncludes index. 311 $a1-118-05469-5 320 $aIncludes bibliographical references at the end of each chapters and index. 327 $aDried Blood Spots; Contents; Preface; Contributors; PART I History, Applications, and Healthcare; 1 Overview of the History and Applications of Dried Blood Samples; 1.1 History; 1.2 Historical Applications; 1.3 Overall Advantages and Disadvantages of Dried-Blood Spot Sampling; 1.3.1 Advantages; 1.3.2 Disadvantages; 1.4 Conclusion; References; 2 Dried Blood Spot Cards; 2.1 Introduction; 2.2 Filter Paper; 2.3 Filter Paper Sources; 2.4 Guidelines and Printing; 2.5 Printing Adaptations; 2.6 Further Innovations in Manufacturing; 2.7 Filter Paper Cards Construction 327 $a2.8 Wraparound Cover and Cassette Construction 2.9 Filter Paper Pre-Treatment; 2.10 The Future of the Filter Paper Card; References; 3 Dried Blood Spot Sample Collection, Storage, and Transportation; 3.1 Introduction; 3.2 General Procedures and Precautions for DBS Sample Collection; 3.2.1 Reagents and Materials; 3.2.2 Biosafety and Infection Control; 3.2.3 Specimen Collection; 3.2.4 DBS Prepared by Other Collection Methods; 3.2.5 Labeling of DBS; 3.2.6 Drying; 3.3 Sample Storage and Transportation; 3.3.1 Packaging and Storage; 3.3.2 Transportation 327 $a3.4 Common Errors in Sample Collection, Storage, and Shipment 3.5 Conclusions; Disclaimer; References; 4 Dried Blood Spot Specimens for Polymerase Chain Reaction in Molecular Diagnostics and Public Health Surveillance; 4.1 Introduction; 4.2 Pioneering DBS Nucleic Acid Extraction Methods for PCR; 4.3 Direct PCR Amplification of Targeted Genomic DNA from DBS; 4.4 Expansion of DBS-PCR from Newborn Screening to Diagnosis of Infectious and other Diseases; 4.5 Application of DBS-PCR in Sequencing-Based Molecular Epidemiology Analyses 327 $a4.6 Application of DBS-PCR for HIV Drug Resistance Surveillance and Monitoring in Resource-Limited Settings4.7 Quantitation of Infectious Agent Particles with DBS-PCR; 4.8 Conclusions; References; 5 Application of Enzyme Immunoassay Methods Using Dried Blood Spot Specimens; 5.1 Introduction; 5.2 Overview of Basic ELISA Systems; 5.2.1 Antibody Detection; 5.2.2 Variation of ELISA Method for Antibody Detection; 5.2.3 Variations of ELISA for Antigen Detection; 5.3 Applications of ELISA Methods Using DBS; 5.4 Optimization and ELISA Methods Validation Using DBS Specimens; 5.4.1 DBS Elution Protocol 327 $a5.4.2 Defining Cutoff Values5.5 Factors Influencing the Performance of DBS-based ELISA Methods; 5.5.1 DBS Preparation; 5.5.2 Anticoagulant; 5.5.3 Punching; 5.5.4 Cross-Contamination; 5.6 Conclusions; Disclaimer; References; 6 Applications of Dried Blood Spots in Newborn and Metabolic Screening; 6.1 Introduction; 6.1.1 History and Chronology in the Use of DBS in NBS; 6.1.2 Chapter Organization and Perspectives; 6.2 Fundamentals of Dried Blood Spots; 6.2.1 A Class II Medical Device; 6.2.2 Liquid versus Dried Blood Specimens; 6.2.3 Interaction of Paper with Biological Matrix and Substrates 327 $a6.2.4 Sample Collection and DBS 330 $aThis book covers the fundamentals, experimental procedures, and applications of dried blood spot (DBS) sampling in combination with various qualitative and quantitative analytical techniques. This includes sample collection, storage, transportation, and sample preparation for various analysis. Experimental examples in newborn screening, toxicokinetics and pharmacokinetics, and forensics are also given. Finally, the book provides an overview of trends in quantitative and qualitative analysis of DBS samples as well as future perspectives. This provides a valuable working guide to researchers, 410 0$aWiley series on pharmaceutical science and biotechnology. 606 $aBlood$xCollection and preservation 606 $aPhlebotomy 606 $aForensic hematology 606 $aForensic serology 615 0$aBlood$xCollection and preservation. 615 0$aPhlebotomy. 615 0$aForensic hematology. 615 0$aForensic serology. 676 $a616.07/561 702 $aLi$b Wenkui$f1964- 702 $aLee$b Mike S.$f1960- 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910823859903321 996 $aDried blood spots$94066754 997 $aUNINA