LEADER 05045nam 2200685Ia 450 001 9910823359103321 005 20240509091356.0 010 $a1-118-06030-X 010 $a1-282-75617-6 010 $a9786612756177 010 $a0-470-87561-5 010 $a0-470-87560-7 035 $a(CKB)2670000000034634 035 $a(EBL)588877 035 $a(OCoLC)667271561 035 $a(SSID)ssj0000411934 035 $a(PQKBManifestationID)11268338 035 $a(PQKBTitleCode)TC0000411934 035 $a(PQKBWorkID)10365228 035 $a(PQKB)10032923 035 $a(MiAaPQ)EBC588877 035 $a(Au-PeEL)EBL588877 035 $a(CaPaEBR)ebr10419244 035 $a(CaONFJC)MIL275617 035 $a(EXLCZ)992670000000034634 100 $a20100615d2010 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aAntigen retrieval immunohistochemistry based research and diagnostics /$fedited by Shan-Rong Shi, Clive R. Taylor 205 $a1st ed. 210 $aHoboken, NJ $cWiley$d2010 215 $a1 online resource (470 p.) 225 1 $aWiley series in biomedical engineering and multi-disciplinary integrated systems. ;$v1 300 $aDescription based upon print version of record. 311 $a0-470-62452-3 320 $aIncludes bibliographical references and index. 327 $aANTIGEN RETRIEVAL IMMUNOHISTOCHEMISTRY BASED RESEARCH AND DIAGNOSTICS; CONTENTS; PREFACE; CONTRIBUTORS; PART I: RECENT ADVANCES IN ANTIGEN RETRIEVAL TECHNIQUES AND ITS APPLICATION; CHAPTER 1: STANDARDIZATION OF ANTIGEN RETRIEVAL TECHNIQUES BASED ON THE TEST BATTERY APPROACH; CHAPTER 2: EXTENDED APPLICATION OF ANTIGEN RETRIEVAL TECHNIQUE IN IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION; CHAPTER 3: EXTRACTION OF DNA/RNA FROM FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE BASED ON THE ANTIGEN RETRIEVAL PRINCIPLE; PART II: STANDARDIZATION OF IMMUNOHISTOCHEMISTRY 327 $aCHAPTER 4: KEY ISSUES AND STRATEGIES OF STANDARDIZATION FOR QUANTIFIABLE IMMUNOHISTOCHEMISTRYCHAPTER 5: STANDARDIZATION OF IMMUNOHISTOCHEMISTRY BASED ON ANTIGEN RETRIEVAL TECHNIQUE; CHAPTER 6: STANDARD REFERENCE MATERIAL: CELL LINE DEVELOPMENT AND USE OF REFERENCE CELL LINES AS STANDARDS FOR EXTERNAL QUALITY ASSURANCE OF HER2 IHC AND ISH TESTING; CHAPTER 7: PEPTIDES AS IMMUNOHISTOCHEMISTRY CONTROLS; CHAPTER 8: STANDARD REFERENCE MATERIAL: PROTEIN-EMBEDDING TECHNIQUE AND DESIGN OF BAR CODE 327 $aCHAPTER 9: THE PROS AND CONS OF AUTOMATION FOR IMMUNOHISTOCHEMISTRY FROM THE PROSPECTIVE OF THE PATHOLOGY LABORATORYCHAPTER 10: IMAGE ANALYSIS IN IMMUNOHISTOCHEMISTRY; PART III: TISSUE/CELL SAMPLE PREPARATION; CHAPTER 11: TISSUE CELL SAMPLE PREPARATION: LESSONS FROM THE ANTIGEN RETRIEVAL TECHNIQUE; CHAPTER 12: MECHANISMS OF ACTION AND PROPER USE OF COMMON FIXATIVES; CHAPTER 13: CELL SAMPLE PREPARATION FOR CLINICAL CYTOPATHOLOGY: CURRENT STATUS AND FUTURE DEVELOPMENT; CHAPTER 14: DESIGN OF A TISSUE SURROGATE TO EXAMINE ACCURACY OF PROTEOMIC ANALYSIS 327 $aPART IV: MOLECULAR MECHANISM OF ANTIGEN RETRIEVAL TECHNIQUECHAPTER 15: STUDY OF FORMALIN FIXATION AND HEAT-INDUCED ANTIGEN RETRIEVAL; CHAPTER 16: A LINEAR EPITOPES MODEL OF ANTIGEN RETRIEVAL; CHAPTER 17: pH OR IONIC STRENGTH OF ANTIGEN RETRIEVAL SOLUTION: A POTENTIAL ROLE FOR REFOLDING DURING HEAT TREATMENT; CHAPTER 18: COMMENTARY: FUTURE DIRECTIONS; PART V: PROTEOMIC ANALYSIS OF PROTEIN EXTRACTED FROM TISSUE/CELLS; CHAPTER 19: TECHNIQUES OF PROTEIN EXTRACTION FROM FFPE TISSUE/ CELLS FOR MASS SPECTROMETRY 327 $aCHAPTER 20: APPLICATION OF SHOTGUN PROTEOMICS TO FORMALIN-FIXED AND PARAFFIN-EMBEDDED TISSUESCHAPTER 21: VISUALIZING PROTEIN MAPS IN TISSUE; CHAPTER 22: SYMBIOSIS OF IMMUNOHISTOCHEMISTRY AND PROTEOMICS: MARCHING TO A NEW ERA; APPENDIX: RELATED LABORATORY PROTOCOLS; INDEX; Color plates 330 $aThe most complete, up-to-date reference on antigen retrieval and immunohistochemistry An antigen is a substance that prompts the generation of antibodies and can cause an immune response. The antigen retrieval (AR) technique is in wide use across the globe, and is a critical technique used in medical diagnosis of disease, particularly clinical targeted cancer treatment. Antigen Retrieval Immunohistochemistry Based Research and Diagnostics discusses several scientific approaches to the standardization of quantifiable immunohistochemistry (IHC). Based on the development and app 410 0$aWiley series in biomedical engineering and multi-disciplinary integrated systems ;$v1. 606 $aImmunohistochemistry 606 $aAntigens 615 0$aImmunohistochemistry. 615 0$aAntigens. 676 $a616.07/56 701 $aShi$b Shan-Rong$f1936-$01715154 701 $aTaylor$b C. R$g(Clive Roy)$01715155 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910823359103321 996 $aAntigen retrieval immunohistochemistry based research and diagnostics$94109546 997 $aUNINA