LEADER 05330nam 2200637 450 001 9910822510503321 005 20230803195620.0 010 $a1-908230-64-9 035 $a(CKB)2670000000547261 035 $a(EBL)1899237 035 $a(SSID)ssj0001216668 035 $a(PQKBManifestationID)11836985 035 $a(PQKBTitleCode)TC0001216668 035 $a(PQKBWorkID)11197427 035 $a(PQKB)11089822 035 $a(MiAaPQ)EBC1899237 035 $a(MiAaPQ)EBC5897796 035 $a(Au-PeEL)EBL5897796 035 $a(OCoLC)876880344 035 $a(EXLCZ)992670000000547261 100 $a20191014d2014 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aMolecular diagnostics $ecurrent research and applications /$fedited by Jim F. Huggett and Justin O'Grady 210 1$aNorfolk, England :$cCaister Academic Press,$d[2014] 210 4$dİ2014 215 $a1 online resource (266 p.) 300 $aDescription based upon print version of record. 311 $a1-908230-41-X 320 $aIncludes bibliographical references and index. 327 $aContents; Contributors; Current books of interest; Foreword; Biographies; 1: Molecular Diagnostics: An Introduction; Part I: Molecular Diagnostics in Cancer: Research and Development of Biomarkers; 2: Transcriptome-based Biomarkers: A Road Map Exemplified for Peripheral Blood-based Biomarker Discovery, Development and Clinical Use; Introduction; Defining biomarker classes as a prerequisite for successful biomarker development; Focus on peripheral blood-based biomarkers as an example; Blood transcriptomics started in leukaemia research 327 $aIs there a role for blood transcriptomics in other diseases?Is there a role of blood transcriptomics for early detection of disease?; General guidance towards successful biomarker development; Importance of technical and organizational aspects during biomarker development; Requirements for bioinformatics analysis during biomarker development; The next decade of blood transcriptomics; 3: Development of Methylation Biomarkers for Clinical Applications and Methylation-sensitive High-resolution Melting Technology; DNA methylation; Methylation-based in vitro diagnostic tests 327 $aFields of applications of in vitro diagnostic testsChallenges of methylation biomarker development process; Methylation biomarker development workflow; PCR amplification and melting analyses in methylation studies; Development of methylation-sensitive high-resolution melting (MS-HRM); Characteristics of the MS-HRM protocol; Final remarks; 4: Genetic and Epigenetic Biomarkers of Colorectal Cancer ; Introduction; Biomarkers; Biomarker location; Practical limitations; Conclusions and outlook; Part II: Molecular Diagnostics of Infectious Diseases: Past, Present and Future 327 $a5: Molecular Diagnosis in Medical Microbiology: The Horizon Draws NearIntroduction; Enhancing diagnosis; Molecular typing in control of infection; Molecular tools in screening/high-throughput analysis; Considerations in selecting a molecular tool; Conclusion; 6: Viral Diagnostics and qPCR-based Methodologies; Introduction; PCR-based methods; Non-PCR-based methods; Perspectives; Validation/standardization/harmonization; Conclusions; 7: XMRV: A Cautionary Tale; Introduction; Discovery of XMRV; Reports of XMRV in prostate cancer and chronic fatigue syndrome 327 $aMounting negative evidence ignites XMRV controversyHuman pathogen or laboratory contaminant?; The recombinant origin of XMRV; De?nouement and conclusions; Note added in proof; 8: Ancient DNA and the Fingerprints of Disease: Retrieving Human Pathogen Genomic Sequences from Archaeological Remains Using Real-time Quantitative Polymerase Chain Reaction; Introduction; aDNA breakdown after deposition; Why study aDNA and what can it tell us?; Sampling and measures to avoid cross-contamination; PCR; Validation of PCR findings; Conclusions; Part III: From Bench to Bedside 327 $a9: Point-of-care Nucleic Acid Testing: User Requirements, Regulatory Affairs and Quality Assurance 330 $aThe application of molecular technology in clinical diagnosis is a rapidly developing area and is predicted to greatly improve the speed, efficiency, and accuracy of diagnostic medicine. The editors of this book have commissioned an excellent series of chapters representing two key molecular diagnostic areas: cancer and infectious diseases. The cancer section deals with the challenges in identifying genetic, epigenetic, and transcriptomic biomarkers. The infectious disease section describes the current clinical applications of molecular diagnostics for the detection of viral, bacterial, and fu 606 $aMolecular diagnosis 606 $aCancer$xMolecular diagnosis 606 $aCommunicable diseases$xMolecular diagnosis 615 0$aMolecular diagnosis. 615 0$aCancer$xMolecular diagnosis. 615 0$aCommunicable diseases$xMolecular diagnosis. 676 $a616.0756 702 $aHuggett$b Jim F. 702 $aO'Grady$b Justin 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910822510503321 996 $aMolecular diagnostics$91409820 997 $aUNINA