LEADER 01549nam 2200517 450 001 9910821075403321 005 20240131205731.0 010 $a84-9148-358-6 035 $a(CKB)4340000000247285 035 $a(MiAaPQ)EBC5307481 035 $a(MiAaPQ)EBC6522176 035 $a(Au-PeEL)EBL6522176 035 $a(OCoLC)989816396 035 $a(OCoLC)1105898825 035 $a(FlNmELB)ELB58890 035 $a(EXLCZ)994340000000247285 100 $a20180516d2017 uy 0 101 0 $aspa 135 $aurcnu|||||||| 181 $2rdacontent 182 $2rdamedia 183 $2rdacarrier 200 10$aConstruccio?n de paz $edisen?o de intervencio?n en conflictos /$fFranco Conforti ; prologado por Johan Galtung 205 $a2a. edicio?n ampliada. 210 1$aMadrid :$cDykinson,$d2017. 215 $a1 online resource (267 pa?ginas) $cilustraciones 311 $a84-9148-308-X 320 $aContiene referencias bibliogra?ficas. 606 $aPacific settlement of international disputes 606 $aPeace 606 $aPaz 606 $aRelaciones internacionales 608 $aLibros electronicos. 615 0$aPacific settlement of international disputes. 615 0$aPeace. 615 4$aPaz. 615 4$aRelaciones internacionales. 676 $a341.52 700 $aConforti$b Franco$01623015 702 $aGaltung$b Johan 801 0$bFINmELB 801 1$bFINmELB 906 $aBOOK 912 $a9910821075403321 996 $aConstruccio?n de Paz$93989521 997 $aUNINA LEADER 03488nam 22006495 450 001 9910983338303321 005 20250114115227.0 010 $a9789819613014 010 $a9819613019 024 7 $a10.1007/978-981-96-1301-4 035 $a(CKB)37203792500041 035 $a(MiAaPQ)EBC31878994 035 $a(Au-PeEL)EBL31878994 035 $a(DE-He213)978-981-96-1301-4 035 $a(OCoLC)1484883055 035 $a(EXLCZ)9937203792500041 100 $a20250114d2025 u| 0 101 0 $aeng 135 $aur||||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aDevelopment of Mirror-Image VHHs with Less Immunogenicity /$fby Keisuke Aoki 205 $a1st ed. 2025. 210 1$aSingapore :$cSpringer Nature Singapore :$cImprint: Springer,$d2025. 215 $a1 online resource (140 pages) 225 1 $aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5061 311 08$a9789819613007 311 08$a9819613000 327 $aChapter 1.Introduction -- Chapter 2.Chemical Synthesis of Mirror-Image VHHs and Evaluation of Their Immunogenicity -- Chapter 3.Identification of a Mirror-Image VHH against Vascular Endothelial Growth Factor -- Chapter 4.Synthetic Study of Full-Length Hepatitis B Virus Core Protein and Its Capsid Assembly -- Chapter 5.Chemical Synthesis of Interleukin-6 for Mirror-Image VHH Discovery -- Chapter 6.Conclusions. 330 $aThis book outlines the chemical synthesis of therapeutic targets and a screening process for unexplored mirror-image single-domain antibodies (D-VHH). This book first describes the chemical synthesis of model VHH and the characteristics of both enantiomeric VHHs, including binding activity, biodistribution and immunogenicity. Immunogenicity testing in mice demonstrated that administration of conventional L-VHH induced the generation of anti-drug antibodies while D-VHH-binding antibodies were not observed in D-VHH-immunized mice. Second, it is explained that the T7-phage-based mirror-image screening system was developed to identify D-VHH antibody fragments capable of binding to vascular endothelial growth factors. Additionally, this book details the chemical synthesis of full-length hepatitis B virus core protein and interleukin-6 for the application of exploring D-VHHs and other mirror-image molecules. Demonstrating that it is possible to develop a D-VHH that preferentially binds the native target protein and exhibits reduced immunogenicity, this book is useful for anyone interested in developing alternative biotherapeutics with reduced immunogenicity by combining chemical and biological approaches. 410 0$aSpringer Theses, Recognizing Outstanding Ph.D. Research,$x2190-5061 606 $aChemistry 606 $aPharmacy 606 $aProteins 606 $aImmunogenetics 606 $aChemical Synthesis 606 $aPharmacy 606 $aProteins 606 $aImmunogenetics 615 0$aChemistry. 615 0$aPharmacy. 615 0$aProteins. 615 0$aImmunogenetics. 615 14$aChemical Synthesis. 615 24$aPharmacy. 615 24$aProteins. 615 24$aImmunogenetics. 676 $a541.39 700 $aAoki$b Keisuke$01785138 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910983338303321 996 $aDevelopment of Mirror-Image VHHs with Less Immunogenicity$94316765 997 $aUNINA