LEADER 06186oam 2200481Mu 450 001 9910820331103321 005 20200110112931.0 010 $a1-4987-5218-7 010 $a1-351-64855-1 010 $a1-315-15251-7 035 $a(CKB)4100000009931103 035 $a(MiAaPQ)EBC5981840 035 $a(OCoLC)1128438324 035 $a(OCoLC-P)1128438324 035 $a(FlBoTFG)9781315152516 035 $a(EXLCZ)994100000009931103 100 $a20191123d2019 uy 0 101 0 $aeng 135 $aurcnu---unuuu 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 14$aThe Giant Vesicle Book$b[electronic resource] 210 $aMilton $cCRC Press LLC$d2019 215 $a1 online resource (xxiii, 652 pages) $cillustrations (some color) 300 $aDescription based upon print version of record. 311 $a1-4987-5217-9 320 $aIncludes bibliographical references and index. 327 $aPreparation methods for giant unilamellar vesicles / Rumiana Dimova, Pasquale Stano, Carlos M. Marques and Peter Walde -- Preparation and properties of giant plasma membrane vesicles and giant unilamellar vesicles from natural membranes / Joseph H. Lorent and Ilya Levental -- Protein reconstitution in giant vesicles / Matthias Garten, Daniel Le?vy and Patricia Bassereau -- GUVs with cytoskeleton / Tobias Ha?rtel and Petra Schwille -- Understanding giant vesicles : a theoretical perspective / Reinhard Lipowsky -- Simulating membranes, vesicles, and cells / Thorsten Auth, Dmitry A. Fedosov and Gerhard Gompper -- Theory of vesicle dynamics in flow and electric fields / Petia M. Vlahovska and Chaouqi Misbah -- Particle-membrane interactions / Jaime Agudo-Canalejo, Reinhard Lipowsky -- Theory of polymer-membrane interactions / Fabrice Thalmann and Carlos M. Marques -- Application of optical microscopy techniques on giant unilamellar vesicles / Luis A. Bagatolli -- Mechanics assays of synthetic lipid membranes based on micropipette aspiration / Elisa Parra and David Needham -- Atomic force microscopy of giant unilamellar vesicles / Andreas Janshoff -- Manipulation and biophysical characterization of GUVs with an optical stretcher / Gheorghe Cojoc, Antoine Girot, Ulysse Delabre and Jochen Guck -- Vesicle fluctuation analysis / John Hjort Ipsen, Allan Grønhøj Hansen and Tripta Bhatia -- Using electric fields to assess membrane material properties in GUVs / Rumiana Dimova and Karin A. Riske -- Creating membrane nanotubes from GUVs / Coline Pre?vost, Mijo Simunovic and Patricia Bassereau -- Measuring GUV adhesion / Kheya Sengupta and Ana Smith -- Phase diagrams and tie lines in GUVs / Matthew C. Blosser, Caitlin Cornell, Scott P. Rayermann and Sarah L. Keller -- Vesicle dynamics in flow: an experimental approach / Victor Steinberg and Michael Levan -- Membrane permeability measurements / Begon?a Ugarte-Uribe, Ana J. Garci?a-Sa?ez and Mireille M.A E. Claessens -- Lipid and protein mobility in GUVs / Begon?a Ugarte-Uribe, Kushal Kumar Das and Ana J. Garci?a-Sa?ez -- Shining light on membranes / Rosangela Itri, Carlos M. Marques and Mauricio S. Baptista -- Protein-membrane interactions / Eva M Schmid and Daniel A Fletcher -- Effects of antimicrobial peptides and detergents on GUVS / Karin A. Riske -- Lipid-polymer interactions: effect on GUVs shapes and behavior / Brigitte Pe?pin-Donat, Franc?ois Quemeneur and Cle?ment Campillo -- Polymersomes / Praful Nair, David Christian and Dennis E. Discher -- Giant hybrid polymer/lipid vesicles / Thi Phuong Tuyen Dao, Khalid Ferji, Fabio Fernandes, Manuel Prieto, Se?bastien Lecommandoux, Emmanuel Ibarboure, Olivier Sandre and Jean-Franc?ois Le Meins -- Giant unilamellar vesicles: from protocell models to the construction of minimal cells / Masayuki Imai and Peter Walde -- Encapsulation of aqueous two-phase systems and gels within giant lipid vesicles / Allyson M. Marianelli and Christine D. Keating -- Droplet-supported giant lipid vesicles as compartments for synthetic biology / Johannes P. Frohnmayer, Marian Weiss, Lucia T. Benk, Jan-Willi Janiesch, Barbara Haller, Rafael B. Lira, Rumiana Dimova, Ilia Plazman and Joachim P. Spatz. 330 $aGiant vesicles are widely used as a model membrane system, both for basic biological systems and for their promising applications in the development of smart materials and cell mimetics, as well as in driving new technologies in synthetic biology and for the cosmetics and pharmaceutical industry. The reader is guided to use giant vesicles, from the formation of simple membrane platforms to advanced membrane and cell system models. It also includes fundamentals for understanding lipid or polymer membrane structure, properties and behavior. Every chapter includes ideas for further applications and discussions on the implications of the observed phenomena towards understanding membrane-related processes. The Giant Vesicle Book is meant to be a road companion, a trusted guide for those making their first steps in this field as well as a source of information required by experts. Key Features A complete summary of the field, covering fundamental concepts, practical methods, core theory, and the most promising applications A start-up package of theoretical and experimental information for newcomers in the field Extensive protocols for establishing the required preparations and assays Tips and instructions for carefully performing and interpreting measurements with giant vesicles or for observing them, including pitfalls Approaches developed for investigating giant vesicles as well as brief overviews of previous studies implementing the described techniques Handy tables with data and structures for ready reference 606 $aLiposomes 606 $aLipid membranes$xBiotechnology 615 0$aLiposomes. 615 0$aLipid membranes$xBiotechnology. 676 $a571.655 700 $aDimova$b Rumiana$c(Scientist)$01605795 701 $aMarques$b Carlos$c(Scientist)$01605796 801 0$bOCoLC-P 801 1$bOCoLC-P 906 $aBOOK 912 $a9910820331103321 996 $aThe Giant Vesicle Book$93931237 997 $aUNINA