LEADER 03266nam 2200493 450 001 9910819518403321 005 20230807202206.0 010 $a3-8325-8790-X 035 $a(CKB)4100000010135086 035 $a(MiAaPQ)EBC6032858 035 $a5e469732-8464-44b2-8003-4e00b0dd2d03 035 $a(EXLCZ)994100000010135086 100 $a20200316d2015 uy 0 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aIdentification of novel modulators towards high cell density and high-producing Chinese hamster ovary suspension cell cultures as well as their application in biopharmaceutical protein production /$fBeat Thalmann 210 1$aBerlin, Germany :$cLogos Verlag,$d[2015] 210 4$dİ2015 215 $a1 online resource (viii, 207 pages) $cillustrations 225 0 $aBielefelder Schriften zur molekularen Biotechnologie ;$v15 300 $a"Dissertation for the completion of the doctoral degree doctor rerum naturalium at the technical faculty of the Bielefeld University, Germany." 311 $a3-8325-4046-6 320 $aIncludes bibliographical references (pages 178-206). 330 $aLong description: Amongst the mammalian producer cell lines, the Chinese hamster ovary (CHO) cell lines are of predominant importance in biopharmaceutical production. Thus, novel factors increasing overall productivity are sought and bear the potential to reduce the unit costs of a production process. Furthermore, the current patent situation for several therapeutic proteins demands innovative tools to at least maintain or preferentially increase the cost-effectiveness of their production processes. In this thesis, hitherto unknown factors were revealed by next generation sequencing of chemically mutated and selected CHO-K1 suspension cell lines. Two factors were proven to improve CHO-based production processes: cgrSnord78 and cgrTtc36. The Cricetulus griseus Ttc36 increases the integral as well as the maximal viable cell density and abolishes the cell-cell aggregation whilst cgrSnord78 improves the specific as well as volumetric productivity without significant impact on cell growth. Based on the present results and discussion, foundations for future research on these functionally unrevealed factors are laid. Hence, this work represents the first step towards the application of the genuine biomolecules cgrTtc36 and cgrSnord78 in biopharmaceutical protein production. 606 $aAnimal cell biotechnology 606 $aCell culture 606 $aOvaries$xPhysiology$xResearch 606 $aHamsters as laboratory animals 615 0$aAnimal cell biotechnology. 615 0$aCell culture. 615 0$aOvaries$xPhysiology$xResearch. 615 0$aHamsters as laboratory animals. 676 $a571.638 700 $aThalmann$b Beat$f1984-$01661194 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910819518403321 996 $aIdentification of novel modulators towards high cell density and high-producing Chinese hamster ovary suspension cell cultures as well as their application in biopharmaceutical protein production$94016970 997 $aUNINA