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100   $a20041028d2005      uy             0
101 0 $aeng
135   $aur|n|---|||||
181   $ctxt
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183   $acr
200 00$aMolecular imaging$b[electronic resource] $eFRET microscopy and spectroscopy /$fedited by Ammasi Periasamy, Richard N. Day
210   $aOxford ;$aNew York $cPublished for the American Physiological Society by Oxford University Press$d2005
215   $a1 online resource (329 p.)
225 1 $aThe American Physiological Society methods in physiology series
300   $aDescription based upon print version of record.
311   $a0-19-517720-7 
320   $aIncludes bibliographical references and index.
327   $aFront Cover; Molecular Imaging: FRET Microscopy and Spectroscopy; Copyright Page; Contents; Contributors; Chapter 1. Proteins and the Flow of Information in Cellular Function; Chapter 2. Basics of Fluorescence and FRET; Chapter 3. An Introduction to Filters and Mirrors for FRET; Chapter 4. FRET Imaging in the Wide-Field Microscope; Chapter 5. Confocal FRET Microscopy: Study of Clustered Distribution of Receptor-Ligand Complexes in Endocytic Membranes; Chapter 6. Multiphoton FRET Microscopy for Protein Localization in Tissue; Chapter 7. FRET Data Analysis: The Algorithm
327   $aChapter 8. Photobleaching FRET MicroscopyChapter 9. Single-Molecule FRET; Chapter 10. FRET Measurements Using Multispectral Imaging; Chapter 11. Real-Time Fluorescence Lifetime Imaging and FRET Using Fast-Gated Image Intensifiers; Chapter 12. Streak Fluorescence Lifetime Imaging Microscopy: A Novel Technology for Quantitative FRET Imaging; Chapter 13. Time-Correlated Single Photon Counting Fluorescence Lifetime Imaging- FRET Microscopy for Protein Localization; Chapter 14. Bioluminescence Resonance Energy Transfer: Techniques and Potential
327   $aChapter 15. Quantifying Molecular Interactions with Fluorescence Correlation SpectroscopyChapter 16. Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Index
330   $aThe detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyz
410  0$aMethods in physiology series.
606   $aFluorescence microscopy
606   $aFluorescence spectroscopy
615  0$aFluorescence microscopy.
615  0$aFluorescence spectroscopy.
676   $a570/.28
701   $aPeriasamy$b Ammasi$01660592
701   $aDay$b Richard N$0128885
712 02$aAmerican Physiological Society (1887- )
801  0$bMiAaPQ
801  1$bMiAaPQ
801  2$bMiAaPQ
906   $aBOOK
912   $a9910817984903321
996   $aMolecular imaging$94015925
997   $aUNINA