LEADER 05288nam 2200649Ia 450 001 9910816845503321 005 20200520144314.0 010 $a1-282-27891-6 010 $a9786612278914 010 $a0-470-44287-5 010 $a0-470-44283-2 035 $a(CKB)1000000000789645 035 $a(EBL)455841 035 $a(OCoLC)609844577 035 $a(SSID)ssj0000354508 035 $a(PQKBManifestationID)11965135 035 $a(PQKBTitleCode)TC0000354508 035 $a(PQKBWorkID)10322577 035 $a(PQKB)11093451 035 $a(MiAaPQ)EBC455841 035 $a(Au-PeEL)EBL455841 035 $a(CaPaEBR)ebr10333033 035 $a(CaONFJC)MIL227891 035 $a(OCoLC)587401945 035 $a(EXLCZ)991000000000789645 100 $a20080814d2009 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aModern size-exclusion liquid chromatography /$fAndre M. Striegel ... [et al.] 205 $a2nd ed. 210 $aHoboken, N.J. $cWiley$dc2009 215 $a1 online resource (512 p.) 300 $aIncludes index. 311 $a0-471-20172-3 327 $aMODERN SIZE-EXCLUSION LIQUID CHROMATOGRAPHY; CONTENTS; Foreword; Preface; 1 Background; 1.1 Introduction; 1.2 History; 1.3 Utility of SEC; 1.4 Molar Mass Averages and Molar Mass Distribution; 1.5 Structure of The Book; References; 2 Retention; 2.1 Introduction; 2.2 Solute Retention in LC; 2.3 Solute Retention in SEC; 2.4 SEC Retention Mechanism; 2.5 Theoretical Models of SEC Separation; 2.5.1 Hard-Sphere Solute Model; 2.5.2 Rigid Molecules of Other Shapes; 2.5.3 Random-Coil Solute Model; 2.6 Other Considerations; 2.6.1 Factors Influencing SEC Retention 327 $a2.6.2 Failure to Define an Effective Polymer Radius2.6.3 Hydrodynamic Chromatography Effects in SEC; 2.6.4 Slalom Chromatography Effects in SEC; References; 3 Band Broadening; 3.1 Introduction; 3.1.1 Basic Column-Dispersion Processes; 3.1.2 Peak Variance; 3.2 LC Plate Theory; 3.2.1 Basic Plate Theory; 3.2.2 The van Deemter Equation; 3.2.3 Flow-Diffusion Coupling; 3.2.4 Reduced Plate Height; 3.3 Mechanism of SEC Band Broadening; 3.3.1 Experimental Verification; 3.3.2 Rate Theory; 3.3.3 Theoretical Inferences; 3.4 Influencing Factors; 3.4.1 Column Parameters; 3.4.2 Kinetic Factors 327 $a3.4.3 Experimental Factors3.5 Experimental Methods; 3.5.1 Plate Number; 3.5.2 Column-Dispersion Calibration; References; 4 Resolution; 4.1 Introduction; 4.1.1 Chromatographic Resolution; 4.1.2 Peak-Capacity Concept; 4.2 Resolution Concept in SEC of Polymers; 4.3 Molar Mass Accuracy Criterion; 4.4 Applications of Column Performance Criteria; 4.5 Pore Geometry and Operational Effects; 4.5.1 Connecting Columns; 4.5.2 Separation Capacity of Single Pores; 4.5.3 Effect of Packing Pore-Size Distribution; 4.5.4 Effect of Operating Parameters; References; 5 Equipment; 5.1 Introduction 327 $a5.2 Extra-Column Effects: General5.3 Mobile-Phase Reservoirs, Inlet Filters, and Degassers; 5.4 Solvent-Metering Systems (Pumps); 5.4.1 General Pump Specifications; 5.4.2 Reciprocating Pumps; 5.5 Sample Injectors and Autosamplers; 5.6 Miscellaneous Hardware; 5.7 Laboratory Safety; References; 6 The Column; 6.1 Introduction; 6.2 Column Packings; 6.2.1 Semirigid Organic Gels; 6.2.2 Rigid Inorganic Packings; 6.3 Column-Packing Methods; 6.3.1 Particle Technology; 6.3.2 Basis of Column-Packing Techniques; 6.4 Column Performance; References; 7 Experimental Variables and Techniques; 7.1 Introduction 327 $a7.2 Solvent Effects7.2.1 Sample Solubility; 7.2.2 Other Solvent Effects; 7.2.3 Flow-Rate Effects; 7.2.4 Temperature Effects; 7.3 Substrate Effects; 7.4 Sample Effects; 7.4.1 Sample Volume; 7.4.2 Sample Weight or Concentration; 7.5 Laboratory Techniques; 7.6 Solvent Selection and Preparation; 7.6.1 Convenience; 7.6.2 Sample Type; 7.6.3 Effect on Column Packing; 7.6.4 Operation; 7.6.5 Safety; 7.6.6 Solvent Purification and Modification; 7.7 Selection and Use of Standard Reference Materials; 7.8 Detector Selection; 7.9 Column Selection and Handling; 7.9.1 Optimum Single Pore-Size Separations 327 $a7.9.2 Bimodal Pore-Size Separations: Optimum Linearity and Range 330 $aThe Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. With its detailed descriptions of techniques, data handling, compilations of information on columns and column packings, and tables of important solvents and reference materials, the book offers readers everything they need to take full advantage of this popular macromolecular characterization technique. Sin 606 $aGel permeation chromatography 606 $aChromatographic analysis 615 0$aGel permeation chromatography. 615 0$aChromatographic analysis. 676 $a543/.8 701 $aStriegel$b Andre M.$f1967-$01716541 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910816845503321 996 $aModern size-exclusion liquid chromatography$94111928 997 $aUNINA