LEADER 03863nam 22006134a 450 001 9910816402303321 005 20230828204647.0 010 $a1-280-73546-5 010 $a9786610735464 010 $a1-84593-148-3 035 $a(CKB)1000000000344590 035 $a(EBL)289448 035 $a(OCoLC)228144308 035 $a(SSID)ssj0000257420 035 $a(PQKBManifestationID)11236981 035 $a(PQKBTitleCode)TC0000257420 035 $a(PQKBWorkID)10228029 035 $a(PQKB)11081408 035 $a(MiAaPQ)EBC289448 035 $a(Au-PeEL)EBL289448 035 $a(CaPaEBR)ebr10157987 035 $a(CaONFJC)MIL73546 035 $a(EXLCZ)991000000000344590 100 $a20050208d2006 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 10$aTesting methods for seed-transmitted viruses$b[electronic resource] $eprinciples and protocols /$fS.E. Albrechtsen 210 $aWallingford, UK ;$aCambridge, MA $cCABI Pub.$dc2006 215 $a1 online resource (280 p.) 300 $aErrata stuck to inside front cover. 311 $a0-85199-016-9 320 $aIncludes bibliographical references and index. 327 $aContents; Preface; Acknowledgements; PART I; 1 Introduction; 1.1. Seed-transmitted Viruses and Viroids; 1.2. The Development of the Science; 1.3. Economic Importance of Seed-transmitted Viruses; 1.4. Testing of Seeds for Viruses: Why?; References; 2 Seed Transmission of Viruses; 2.1. Location of Inoculum in the Seed; 2.2. Plant-to-seed and Seed-to-plant Transmission; References; 3 Ecology, Epidemiology and Control; 3.1. Ecology and Epidemiology; 3.2. Control Strategies; References; PART II; 4 Biological Assays; 4.1. Symptomatology; 4.2. Facilities and Equipment for Biological Assays 327 $a4.3. Growing-on Tests4.4. Infectivity Assays; 4.5. Maintenance of Isolates; References; 5 Serological Testing Methods; 5.1. Antigens and Antibodies; 5.2. Enzyme-linked Immunosorbent Assay (ELISA); 5.3. Dot Immunobinding Assay (DIBA); 5.4. Tissue Blotting Immunoassay (TBIA); 5.5. Other Serological Test Methods; References; 6 Nucleic Acid-based Testing Methods; 6.1. Nucleic Acid Hybridization; 6.2. Enzymatic Nucleic Acid Amplification; References; 7 Epilogue; 7.1. Other Detection Techniques; 7.2. Organization and Interpretation of Seed-health Assays 327 $a7.3. Tolerance Levels of Infection, and Pathogens in Germplasm7.4. Standardization and Cost of Tests; References; Appendix 1. List of Seed-transmitted Viruses and Viroids; Appendix 2. Reagents, Solutions and Buffers; Appendix 3. Suppliers of Laboratory Equipment and Materials; Index; A; B; C; D; E; F; G; H; I; K; L; M; N; O; P; Q; R; S; T; U; V; W; Y; Z 330 $aYield losses and reduced crop quality, caused by plant viruses are an increasing problem. Several important plant viruses are, in addition to their sap and vector transmission, also transmitted through seed. Seed transmission of plant pathogens plays an important role for the early outbreak of disease in a crop, for the survival of inoculum from one crop season to the next, and for its international dispersal. Freedom from seedborne viral infection can be revealed by certain seed health testing methods. This book provides a practical guide to the commonly used detection methods for seed-transm 606 $aSeeds$xTesting 606 $aSeed pathology 606 $aVirus diseases of plants 615 0$aSeeds$xTesting. 615 0$aSeed pathology. 615 0$aVirus diseases of plants. 676 $a632/.8 700 $aAlbrechtsen$b S. E$g(Sven Erik)$0523407 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910816402303321 996 $aTesting methods for seed-transmitted viruses$9827093 997 $aUNINA