LEADER 05181nam 2200637Ia 450 001 9910143129503321 005 20170810175612.0 010 $a1-282-29205-6 010 $a9786612292057 010 $a3-527-62668-9 010 $a3-527-62669-7 035 $a(CKB)1000000000767195 035 $a(EBL)481485 035 $a(OCoLC)437416793 035 $a(SSID)ssj0000137586 035 $a(PQKBManifestationID)11159659 035 $a(PQKBTitleCode)TC0000137586 035 $a(PQKBWorkID)10088690 035 $a(PQKB)11399282 035 $a(MiAaPQ)EBC481485 035 $a(PPN)194575683 035 $a(EXLCZ)991000000000767195 100 $a20080107d2009 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aDetection of highly dangerous pathogens$b[electronic resource] $emicroarray methods for BSL 3 and BSL 4 agents /$fedited by Tanja Kostic, Patrick Butaye, and Jacques Schrenzel 210 $aWeinheim $cWiley-VCH$d2009 215 $a1 online resource (193 p.) 300 $aDescription based upon print version of record. 311 $a3-527-32275-2 320 $aIncludes bibliographical references and index. 327 $aDetection of Highly Dangerous Pathogens: Microarray Methods for the Detection of BSL 3 and BSL 4 Agents; Contents; This Publication is Supported by COST; Preface; List of Contributors; 1 Introduction to Microarray-Based Detection Methods; 1.1 Introduction to Microarray Technology; 1.2 Technical Aspects of Microarray Technology; 1.2.1 Probes; 1.2.1.1 Genome Fragments; 1.2.1.2 PCR Products; 1.2.1.3 Oligonucleotide Probes; 1.2.2 Substrates for Printing; 1.2.2.1 Slides with Poly-L-lysine Coating; 1.2.2.2 Slides with Amino Silane Coating; 1.2.2.3 Slides with Aldehyde Coating 327 $a1.2.2.4 Slides with Epoxy Coating1.2.2.5 Proprietary Surface Chemistries; 1.2.2.6 Probe Spacers; 1.2.3 Targets for Microarray Analysis; 1.2.3.1 Target Amplifications and Sensitivity Issues; 1.2.3.2 Labeling of the Targets; 1.2.3.3 Hybridization and Wash Conditions; 1.2.4 Classical Commercially Available Microarray Formats; 1.2.4.1 Spotting Approaches; 1.2.4.2 In Situ Synthesis; 1.2.5 Alternative Methods for Improving Microarray-Based Detection Sensitivity; 1.2.5.1 Resonance-Light Scattering (RLS); 1.2.5.2 Planar-Waveguide Technology (PWT); 1.2.5.3 Liquid Arrays 327 $a1.2.5.4 Three-Dimensional Microarray Formats1.2.6 Marker Genes Used on MDMs; 1.3 Analysis and Quality Control Aspects; 1.4 Applications of Microarray Technology in Microbial Diagnostics; 1.4.1 Gene Expression Studies; 1.4.2 Comparative Genomic Hybridization (CGH); 1.4.3 Generic or Universal Microarrays; 1.4.4 Microarrays for Sequence Analysis; 1.4.5 Microbial Diagnostic Microarrays (MDMs); 1.5 Further Developments and New Perspectives Regarding Array Sensitivity and Specificity; 1.6 Conclusions; References; Part I: Methods; 2 Long Oligonucleotide Microarray-Based Microbial Detection 327 $a2.1 Introduction2.2 Method; 2.2.1 DNA Extraction; 2.2.2 ?29 Amplification; 2.2.3 Klenow Amplification/Labeling; 2.2.4 Probe and Slide Preparation; 2.2.5 Slide Processing Protocol (for Amino Surfaces); 2.2.6 Hybridization and Slide Washing; 2.2.7 Comments; 2.3 Our Test System and Results; 2.4 Conclusions; References; 3 Sequence-Specific End-Labeling of Oligonucleotides; 3.1 Introduction; 3.2 Probe Design; 3.3 Slide Preparation (Spotting); 3.4 Slide Processing Protocol ( for Aldehyde Surfaces); 3.5 DNA Extraction and PCR Amplification of the Targeted Gene 327 $a3.6 Shrimp Alkaline Phosphatase Treatment3.7 Labeling; 3.8 Hybridization and Slide Washing; 3.9 Data Analysis; 3.10 Costs; 3.11 Microarray for Detection of Pathogenic Bacteria; References; 4 Non-Cognate Approaches for Pathogen Detection on Microarrays; 4.1 Introduction; 4.2 Non-Cognate Hybridization System; 4.2.1 Concept; 4.2.2 Definition of the Optimal Probe Length; 4.2.3 Virtual Assessment of Array Performances (in Silico Experiments); 4.2.4 Array Manufacturing and Hybridization (Wet-Lab Experiments); 4.2.5 Analysis; 4.3 Perspectives; References; 5 Patterning Techniques for Array Platforms 327 $a5.1 Introduction 330 $aWritten by leading experts in the field as part of an interdisciplinary pan-European research program funded by the EU, the results provided in this booklet provide a unique and comprehensive overview of how microarray technology can be used in safely tracking the most highly dangerous pathogens. A must-have for public health agencies focused on bioterrorism as well as all laboratories working with BSL3 and/or BSL 4 agents. 606 $aPathogenic microorganisms$xDetection 606 $aDNA microarrays 615 0$aPathogenic microorganisms$xDetection. 615 0$aDNA microarrays. 676 $a579.165 701 $aKostic$b Tanja$0993881 701 $aButaye$b Patrick$0993882 701 $aSchrenzel$b Jacques$0993883 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910143129503321 996 $aDetection of highly dangerous pathogens$92275740 997 $aUNINA LEADER 03048nam 22004573u 450 001 9910791786403321 005 20230725021009.0 010 $a1-283-01577-3 010 $a9786613015778 010 $a1-4411-3734-3 035 $a(CKB)2560000000060856 035 $a(EBL)661049 035 $a(OCoLC)705538277 035 $a(MiAaPQ)EBC661049 035 $a(EXLCZ)992560000000060856 100 $a20130418d2011|||| u|| | 101 0 $aeng 135 $aur|n|---||||| 200 10$aCognitive Explorations of Translation$b[electronic resource] 210 $aLondon $cContinuum International Publishing$d2011 215 $a1 online resource (253 p.) 225 1 $aContinuum Studies in Translation 300 $aDescription based upon print version of record. 311 $a1-4411-7268-8 327 $aTable of Contents; Series Editor's Preface; Introduction; 1 Back to Basics: Designing a Study to Determine the Validity and Reliability of Verbal Report Data on Translation Processes; 2 Results of the Validation of the PACTE Translation Competence Model: Translation Project and Dynamic Translation Index; 3 Exploring Translation Competence Acquisition: Criteria of Analysis Put to the Test; 4 Development of Translation Competence in Novices: a Corpus Design and Key-Logging Analysis; 5 Uncertainty Management, Metacognitive Bundling in Problem Solving, and Translation Quality 327 $a6 EEG, EYE and Key: Three Simultaneous Streams of Data for Investigating the Cognitive Mechanisms of Translation7 Translation Directionality and the Revised Hierarchical Model: An Eye-Tracking Study; 8 Towards an Investigation of Reading Modalities in/for Translation: an Exploratory Study Using Eye-Tracking Data; 9 Cognitive Effort in Metaphor Translation: an Eye-Tracking Study; 10 Distribution of Attention Between Source Text and Target Text During Translation; 11 Glossary of Terms; Index 330 $aCognitive Explorations of Translation focuses on the topic of investigating translation processes from a cognitive perspective. With little published on this topic to date, Sharon O'Brien brings together a global collection of contributors covering a range of topics. Central themes include modelling translation competence, construction and reformulation of text meaning, translators' behaviour during translation and what methodologies can best be utilized to investigate these topics. Techniques covered include eye-tracking, Think-Aloud protocols, keyboard logging and EEG (Electroencephalogram) 410 0$aContinuum Studies in Translation 606 $aCognitive psychology 606 $aTranslating and interpreting -- Psychological aspects 615 4$aCognitive psychology. 615 4$aTranslating and interpreting -- Psychological aspects. 676 $a418/.02019 700 $aO'Brien$b Sharon$0734563 801 0$bAU-PeEL 801 1$bAU-PeEL 801 2$bAU-PeEL 906 $aBOOK 912 $a9910791786403321 996 $aCognitive Explorations of Translation$93706213 997 $aUNINA LEADER 02617nam 2200493 450 001 9910814852203321 005 20230807193053.0 010 $a1-4629-1716-X 035 $a(CKB)3710000000470419 035 $a(EBL)2197501 035 $a(SSID)ssj0001543375 035 $a(PQKBManifestationID)16133341 035 $a(PQKBTitleCode)TC0001543375 035 $a(PQKBWorkID)14558292 035 $a(PQKB)11101850 035 $a(MiAaPQ)EBC2197501 035 $a(Au-PeEL)EBL2197501 035 $a(OCoLC)919684253 035 $a(EXLCZ)993710000000470419 100 $a20181231d2015 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 12$aA brief history of Indonesia $eSultans, spices, and tsunamis : the incredible story of Southeast Asia's largest nation /$fTim Hannigan 210 1$aTokyo ;$aRutland, Vermont ;$aSingapore :$cTuttle Publishing,$d[2015] 210 4$dİ2015 215 $a1 online resource (321 p.) 300 $aDescription based upon print version of record. 311 $a0-8048-4476-3 320 $aIncludes bibliographical references (pages 280-282) and index. 327 $aPoint of departure : the archipelago -- From hobbits to Hinduism : prehistory to Indianisation -- Empires of imagination : Hindu-Buddhist Java -- Saints and winners : the arrival of Islam -- Spice invaders : the Europeans arrive -- The clash of civilisations : from company to empire -- Rust en orde : the Dutch East Indies -- Brave new world : the rise of nationalism -- Freedom or death : war and revolution -- Years of living dangerously : the Sukarno era -- A new order : Suharto, crisis, reform -- Epilogue : Indonesia, rising. 330 $aSultans, Spices, and Tsunamis: The Incredible Story of the World's Largest ArchipelagoIndonesia is by far the largest nation in Southeast Asia and has the fourth largest population in the world after the United States. Indonesian history and culture are especially relevant today as the Island nation is an emerging power in the region with a dynamic new leader. It is a land of incredible diversity and unending paradoxes that has a long and rich history stretching back a thousand years and more.Indonesia is the fabled ""Spice Islands"" of every school child's dreamsone of the most colorful and f 607 $aIndonesia$xHistory 676 $a959.8 700 $aHannigan$b Tim$0719692 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910814852203321 996 $aA brief history of Indonesia$93926289 997 $aUNINA