LEADER 03275nam 22006372 450 001 9910814117003321 005 20230815161702.0 010 $a1-107-11136-6 010 $a9786610154746 010 $a1-280-15474-8 010 $a0-511-14910-7 010 $a0-511-04996-X 010 $a0-511-11853-8 010 $a0-511-54514-2 010 $a0-511-30946-5 035 $a(CKB)111056485615976 035 $a(EBL)144670 035 $a(OCoLC)437250301 035 $a(SSID)ssj0000178003 035 $a(PQKBManifestationID)11169487 035 $a(PQKBTitleCode)TC0000178003 035 $a(PQKBWorkID)10221258 035 $a(PQKB)11710983 035 $a(UkCbUP)CR9780511545146 035 $a(Au-PeEL)EBL144670 035 $a(CaPaEBR)ebr5005960 035 $a(CaONFJC)MIL15474 035 $a(MiAaPQ)EBC144670 035 $a(PPN)261293850 035 $a(EXLCZ)99111056485615976 100 $a20090507d2000|||| uy 0 101 0 $aeng 135 $aur||||||||||| 181 $ctxt$2rdacontent 182 $cc$2rdamedia 183 $acr$2rdacarrier 200 10$aIn vitro fertilization /$fKay Elder, Brian Dale 205 $aSecond edition. 210 1$aCambridge :$cCambridge University Press,$d2000. 215 $a1 online resource (xii, 310 pages) $cdigital, PDF file(s) 311 0 $a0-521-77863-8 311 0 $a0-511-00602-0 320 $aIncludes bibliographical references and index. 327 $aPreliminaries; Contents; Preface; Acknowledgments; 1 Introduction; 2 Producing gametes; 3 Sperm-oocyte interaction; 4 First stages of development; 5 Endocrine control of reproduction; 6 Assisted reproductive technology in farm animals; 7 The clinical in vitro fertilization laboratory; 8 Semen analysis and preparation for assisted reproductive techniques; 9 Oocyte retrieval and embryo culture; 10 Cryopreservation; 11 Micromanipulation techniques; 12 Preimplantation genetic diagnosis; Index 330 $aThis comprehensively updated and expanded second edition builds on its successful and popular predecessor, retaining the practical features which made the first edition such an essential guide to IVF. The edition describes additions to the range of ART clinical treatments, including the use of testicular and epididymal sperm, blastocyst stage transfer, and new perspectives in cryobiology and cryopreservation techniques. By incorporating laboratory techniques and protocols, with an even greater emphasis on quality control, it provides an indispensable and practical account. The introductory chapters covering the scientific background that underpins effective laboratory practice have been substantially expanded, derived from research in mammalian systems into the molecular biology of oogenesis, oocyte maturation, and early embryo metabolism. This second edition distils a wealth of practical and scientific detail for the benefit of all IVF practitioners. 606 $aFertilization in vitro, Human 615 0$aFertilization in vitro, Human. 676 $a618.1/78059 700 $aElder$b Kay$f1946-$01703625 702 $aDale$b Brian 801 0$bUkCbUP 801 1$bUkCbUP 906 $aBOOK 912 $a9910814117003321 996 $aIn vitro fertilization$94088964 997 $aUNINA