LEADER 05254nam 2200661 a 450 001 9910813672203321 005 20240516184316.0 010 $a1-118-39411-9 010 $a1-118-39414-3 010 $a1-283-70546-X 010 $a1-118-39412-7 035 $a(CKB)2670000000271784 035 $a(EBL)947707 035 $a(OCoLC)793099641 035 $a(SSID)ssj0000754625 035 $a(PQKBManifestationID)11496612 035 $a(PQKBTitleCode)TC0000754625 035 $a(PQKBWorkID)10726154 035 $a(PQKB)11484016 035 $a(MiAaPQ)EBC947707 035 $a(DLC) 2012018153 035 $a(Au-PeEL)EBL947707 035 $a(CaPaEBR)ebr10613590 035 $a(CaONFJC)MIL401796 035 $a(EXLCZ)992670000000271784 100 $a20120502d2013 uy 0 101 0 $aeng 135 $aur|n|---||||| 181 $ctxt 182 $cc 183 $acr 200 00$aExtremophiles $esustainable resources and biotechnological implications /$fedited by Om V. Singh 205 $a1st ed. 210 $aHoboken, N.J. $cWiley-Blackwell$dc2013 215 $a1 online resource (472 p.) 300 $aIncludes index. 311 $a1-118-10300-9 320 $aIncludes bibliographical references and index. 327 $aEXTREMOPHILES; Contents; Contributors; Introduction; 1 MOLECULAR EVOLUTION OF EXTREMOPHILES; 1.1 Introduction; 1.2 Molecular Evolution of Thermophiles; 1.2.1 Habitat; 1.2.2 Cellular Organization; 1.2.3 Genome; 1.2.4 Proteome; 1.3 Molecular Evolution of Psychrophiles; 1.3.1 Habitat; 1.3.2 Cellular Organization; 1.3.3 Genome; 1.3.4 Proteome; 1.4 Molecular Evolution of Halophiles; 1.4.1 Habitat; 1.4.2 Cellular Organization; 1.4.3 Genome; 1.4.4 Proteome; 1.5 Molecular Evolution of Alkaliphiles; 1.5.1 Habitat; 1.5.2 Cellular Organization; 1.5.3 Genome; 1.5.4 Proteome 327 $a1.6 Molecular Evolution of Acidophiles1.6.1 Habitat; 1.6.2 Cellular Organization; 1.6.3 Genome; 1.6.4 Proteome; 1.7 Molecular Evolution of Barophiles; 1.7.1 Habitat; 1.7.2 Cellular Organization; 1.7.3 Genome; 1.7.4 Proteome; 1.8 Engineering Extremophiles; 1.8.1 Microbiology; 1.8.2 Molecular Biology; 1.8.3 Bioinformatics; 1.9 Case Studies; 1.9.1 Biofuel Production; 1.9.2 Bioremediation; 1.9.3 Pesticide Biodegradation; 1.9.4 Escherichia coli: A Candidate Extremophile; 1.9.5 Oil-Spill-Cleaning Bacteria; 1.9.6 Potential Applications and Benefits 327 $a1.10 Implications of Engineered Extremophiles on Ecology, Environment, and Health1.11 Conclusions and Recommendations; References; 2 ATTAINING EXTREMOPHILES AND EXTREMOLYTES: METHODOLOGIES AND LIMITATIONS; 2.1 Introduction; 2.2 Extremophiles: Types and Diversity; 2.2.1 Thermophiles; 2.2.2 Psychrophiles; 2.2.3 Halophiles; 2.2.4 Alkaliphiles; 2.2.5 Acidophiles; 2.2.6 Barophiles; 2.3 Extremolytes; 2.3.1 Production and Purification of Extremolytes; 2.3.2 Detection, Identification, and Quantification of Extremolytes; 2.3.3 Limitations; 2.4 Conclusions; References 327 $a3 STRATEGIES FOR THE ISOLATION AND CULTIVATION OF HALOPHILIC MICROORGANISMS3.1 Introduction; 3.2 Thalassohaline and Athalassohaline Hypersaline Environments; 3.3 Case Studies; 3.3.1 Isolation of Aerobic Chemoheterotrophic Archaea from Solar Salterns; 3.3.2 Magnesium-Requiring and Magnesium-Tolerant Archaea from the Dead Sea; 3.3.3 Isolation of Acidophilic Halophilic Archaea; 3.3.4 Isolation of Unusual Anaerobic Halophiles from Deep-Sea Brines; 3.3.5 Isolation of Polyextremophilic Anaerobic Halophiles; 3.3.6 Isolation of Halophilic Microorganisms Associated with Plants and Animals 327 $a3.3.7 Isolation of Halophilic Archaea from Low-Salt Environments3.4 The Upper Salinity Limits of Different Types of Energy Generation; 3.5 Final Comments; References; 4 HALOPHILIC PROPERTIES AND THEIR MANIPULATION AND APPLICATION; 4.1 Introduction; 4.2 Industrial Applications of Halophilic Organisms and Their Proteins; 4.3 Extreme and Moderate Halophiles and Their Proteins; 4.4 Generation of Low-Salt Stable Extreme-Halophilic Proteins; 4.5 Interconversion of Halophilic and Nonhalophilic Proteins; 4.5.1 Dimer-Tetramer Conversion of HaNDK and PaNDK; 4.5.2 Generation of Halophilic PaNDK 327 $a4.6 Soluble Expression of Recombinant Proteins 330 $a Explores the utility and potential of extremophiles in sustainability and biotechnology Many extremophilic bio-products are already used as life-saving drugs. Until recently, however, the difficulty of working with these microbes has discouraged efforts to develop extremophilic microbes as potential drug reservoirs of the future. Recent technological advances have opened the door to exploring these organisms anew as sources of products that might prove useful in clinical and environmental biotechnology and drug development. Extremophiles features outstanding articl 606 $aExtreme environments$xMicrobiology 606 $aMicrobial biotechnology 615 0$aExtreme environments$xMicrobiology. 615 0$aMicrobial biotechnology. 676 $a578.75/8 701 $aSingh$b Om V$0634269 801 0$bMiAaPQ 801 1$bMiAaPQ 801 2$bMiAaPQ 906 $aBOOK 912 $a9910813672203321 996 $aExtremophiles$94050657 997 $aUNINA