LEADER 05524nam 22007333u 450 001 9910811595603321 005 20240313201820.0 010 $a1-118-57438-9 010 $a1-118-57466-4 010 $a1-118-57434-6 035 $a(CKB)2670000000353530 035 $a(EBL)1175205 035 $a(OCoLC)843199464 035 $a(SSID)ssj0000904860 035 $a(PQKBManifestationID)11582015 035 $a(PQKBTitleCode)TC0000904860 035 $a(PQKBWorkID)10923896 035 $a(PQKB)11746491 035 $a(MiAaPQ)EBC1175205 035 $a(EXLCZ)992670000000353530 100 $a20131118d2013|||| u|| | 101 0 $aeng 135 $aurcnu|||||||| 181 $ctxt 182 $cc 183 $acr 200 10$aOptics in Instruments$b[electronic resource] $eApplications in Biology and Medicine 205 $a1st ed. 210 $aHoboken $cWiley$d2013 215 $a1 online resource (244 p.) 225 1 $aISTE 300 $aDescription based upon print version of record. 311 $a1-84821-244-5 327 $aCover; Title Page; Contents; Preface; Introduction; Chapter 1. Confocal Laser Scanning Microscopy; 1.1. Introduction; 1.1.1. Context and framework of chapter; 1.1.2. From wide-field microscopy to confocal microscopy; 1.2. Principle and implementation; 1.2.1. General principle; 1.2.2. Axial and lateral resolution in confocal microscopy; 1.2.3. Some notions of fluorescence; 1.2.4. Main elements of a confocal scanning laser microscope; 1.3. Applications in biology, potential and limitations; 1.3.1. Basic elements of biology for the neophyte; 1.3.2. Fluorescent labeling 327 $a1.3.3. Practical implementation of confocal microscopy1.4. Related and derived techniques; 1.4.1. Advanced contrast modes: FRAP, FLIP, FLIM, FRET, etc; 1.4.2. The contribution of nonlinear contrast modes; 1.4.3. Recent major advances: overcoming the diffraction limit; 1.5. Bibliography; Chapter 2. Flow Cytometry (FCM) Measurement of Cells in Suspension; 2.1. History of FCM; 2.2. Components of the cytometer: fluidics, optics and signal processing; 2.2.1. Fluidics; 2.2.2. Optics; 2.2.3. Signal processing; 2.3. Experimentation strategy; 2.3.1. Visualizations of the spectra 327 $a2.3.2. Compensation of fluorescences 2.3.3. Checking the optical bench; 2.3.4. Presentation of parameters A/H/W; 2.3.5. Graphical presentation; 2.4. Types of platform for FCM; 2.4.1. Clinical platform; 2.4.2. Research platform; 2.5. Principle of cell sorting; 2.6. Analyzed parameters; 2.6.1. Light scattering; 2.6.2. Fluorochromes; 2.7. Applications in biology; 2.7.1. Clinical; 2.7.2. Research; 2.7.3. Environment; 2.7.4. Plant biology; 2.7.5. Industrial microbiology; 2.8. Complementarities of the FCM with the other cytometries, confocal and dynamic; 2.9. Cytometry on beads, LUMINEXTM type 327 $a2.10. Scientific societies 2.11. Websites to visit; 2.12. Bibliography; 2.13. Reference books; Chapter 3. Optical Coherence Tomography; 3.1. Introduction; 3.2. Principles of OCT; 3.3. Frequency-domain OCT; 3.4. Spatial resolution; 3.5. Applications of OCT; 3.5.1. Ophthalmology; 3.5.2. Internal medicine; 3.5.3. Other fields of application; 3.6. Extensions of OCT; 3.7. Full-field OCT; 3.7.1. Principle; 3.7.2. Spatial resolution; 3.7.3. Dynamics and sensitivity; 3.7.4. Operating speed; 3.7.5. Applications; 3.8. Conclusion; 3.9. Bibliography; Chapter 4. Therapeutic Applications of Lasers 327 $a4.1. Introduction 4.2. Interaction of light with biological tissues; 4.2.1. Optical parameters characterizing light radiation; 4.2.2. The three types of interaction between a light beam and a biological tissue; 4.2.3. Penetration of light in biological tissues; 4.3. Therapeutic effects of lasers; 4.3.1. Thermal effect; 4.3.2. Photoablative effect; 4.3.3. Photochemical or photodynamic effect; 4.3.4. The electromechanical effect; 4.4. Conclusion; 4.5. For more information; 4.6. Bibliography; Chapter 5. Plasmonics; 5.1. Propagating surface plasmons; 5.1.1. Theoretical reminders and definitions 327 $a5.1.2. Surface plasmon resonance sensors 330 $aOptics is a science which covers a very large domain and is experiencing indisputable growth. It has enabled the development of a considerable number of instruments, the optical component or methodology of which is often the essential part of portent systems. This book sets out show how optical physical phenomena such as lasers - the basis of instruments of measurement - are involved in the fields of biology and medicine.Optics in Instruments: Applications in Biology and Medicine details instruments and measurement systems using optical methods in the visible and near-infrared, 410 0$aISTE 606 $aOptical instruments -- Equipment and supplies 606 $aOptics 606 $aOptoelectronic devices 606 $aOptical instruments$xEquipment and supplies 606 $aOptoelectronic devices 606 $aOptics 615 4$aOptical instruments -- Equipment and supplies. 615 4$aOptics. 615 4$aOptoelectronic devices. 615 0$aOptical instruments$xEquipment and supplies 615 0$aOptoelectronic devices 615 0$aOptics 676 $a681.4 676 $a681/.4 700 $aGoure$b Jean Pierre$01655786 702 $aGoure$b J.-P 801 0$bAU-PeEL 801 1$bAU-PeEL 801 2$bAU-PeEL 906 $aBOOK 912 $a9910811595603321 996 $aOptics in Instruments$94008307 997 $aUNINA